Skip to main content

Advertisement

SpringerLink
Log in

Recombinant VP2 expressed in baculovirus and adjuvanted with TIR-TLR7: a vaccine candidate against infectious bursal disease virus

  • Original Article
  • Published:
Comparative Clinical Pathology Aims and scope Submit manuscript

Abstract

Currently, a variety of immunostimulatory molecules are used as adjuvants in combination with poor immunogenic recombinant infectious bursal disease virus (IBDV) vaccine antigen. TIR domain of Toll-like receptors (TLRs) triggers the interleukin-1 (IL-1) signaling cascade which plays a critical role in immune responses against infectious agents. For this study, the bacmid DNA pBac-VP2 of a field isolate was generated and transfected to Sf9 insect cells to produce recombinant VP2 (rVP2). In addition, based on in silico analysis, the cDNA encoding the conserved TIR domain of TLR7 (TIR-TLR7) contain the IL-1 receptor was synthesized and used as an adjuvant. We further evaluated the ability of rVP2/TIR-TLR7 vaccine candidate to induce specific immune responses in specific pathogen-free (SPF) chickens and compared the efficacy by challenging with the virulent IBDV. The results indicate that rVP2/TIR-TLR7-adjuvanted vaccine elicited humoral immune responses and protected chickens against IBDV infection. Higher levels of neutralizing IBDV antibody titers were observed in chickens intramuscularly immunized with rVP2/TIR-TLR7 than those injected with rVP2 alone. The antibody titers were higher in chickens which received booster injection. Furthermore, chickens immunized with rVP2/TIR-TLR7 in the prime and boost groups had significant protection against a virus challenge. The survival rate was 70 and 90% after the primary and booster rVP2/TIR-TLR7 vaccinations, respectively, and indicated that they were protected from virus challenge. Despite the unvaccinated control group, the immunized chickens with rVP2/TIR-TLR7 did not show any clinical signs and histopathological changes of the bursa. Our results demonstrate that TIR-TLR7 is a potential vaccine adjuvant and can induce antigen-specific immune responses induced by rVP2 for protection against IBDV infection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Asif M, Jenkins KA, Hilton LS, Kimpton WJ, Bean AG, Lowenthal JW (2004) Cytokines as adjuvants for avian vaccines. Immunol Cell Biol 82:638–643

    Article  PubMed  CAS  Google Scholar 

  • Bao M, Liu YJ (2000) Regulation of TLR7/9 signaling in plasmacytoid dendritic cells. Protein Cell 4:40–52

    Article  CAS  Google Scholar 

  • Berg TPVD (2000) Acute infectious bursal disease in poultry: a review. Avian Pathol. 29:175–194

    Article  PubMed  CAS  Google Scholar 

  • Colonna M (2007) TLR pathways and IFN-regulatory factors: to each its own. Eur J Immunol 37:306–309

    Article  PubMed  CAS  Google Scholar 

  • Ebrahimi MM, Shahsavandi S, Shayan P, Goudarzi H, Masoudi S (2016) An immunoinformatic assay to design bio adjuvanted vaccine against infectious bursal disease virus. J Biol Today's World 5:13–19

    Article  CAS  Google Scholar 

  • Gomez E, Lucero MS, Chimeno Zoth S, Carballeda JM, Gravisaco MJ, Berinstein A (2013) Transient expression of VP2 in Nicotiana benthamiana and its use as a plant-based vaccine against infectious bursal disease virus. Vaccine 31:2623–2627

    Article  PubMed  CAS  Google Scholar 

  • Gupta SK, Deb R, Dey S, Chellappa MM (2014) Toll-like receptor-based adjuvants: enhancing the immune response to vaccines against infectious diseases of chicken. Expert Rev Vaccines 13:909–925

    Article  PubMed  CAS  Google Scholar 

  • Haller O, Kochs G, Weber F (2006) The interferon response circuit: induction and suppression by pathogenic viruses. Virology 344:119–130

    Article  PubMed  CAS  Google Scholar 

  • Honda K, Yanai H, Negishi H, Asagiri M, Sato M, Mizutani T, Shimada N, Ohba Y, Takaoka A, Yoshida N, Taniguchi T (2005) IRF-7 is the master regulator of type-I interferon-dependent immune responses. Nature 434:772–777

    Article  PubMed  CAS  Google Scholar 

  • Huang Z, Elankumaran S, Yunus AS, Samal SK (2004) A recombinant Newcastle disease virus (NDV) expressing VP2 protein of infectious bursal disease virus (IBDV) protects against NDV and IBDV. J Virol 78:10054–10063

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kannaki T, Reddy M, Shanmugam M, Verma P, Sharma R (2010) Chicken toll-like receptors and their role in immunity. World’s Poult Sci J 66:727–738

    Article  Google Scholar 

  • Kong N, Wang X, Zhao J, Hu J, Zhang H, Zhao H (2011) A recombinant baculovirus expressing VP2 protein of infectious bursal disease virus (IBDV) and chicken interleukin-18 (ChIL-18) protein protects against very virulent IBDV. J Anim Vet Adv 10:2706–2715

    CAS  Google Scholar 

  • Lee HJ, Kim JY, Kye SJ, Seul HJ, Jung SC, Choi KS (2015) Efficient self-assembly and protective efficacy of infectious bursal disease virus-like particles by a recombinant baculovirus co-expressing precursor polyprotein and VP4. Virology J 12:177. https://doi.org/10.1186/s12985-015-0403-4

    Article  CAS  Google Scholar 

  • Li J, Liang X, Huang Y, Meng S, Xie R, Deng R, Yu L (2004) Enhancement of the immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with plasmid encoding chicken interleukin 2. Virology 329:89–100

    Article  PubMed  CAS  Google Scholar 

  • Liang J, Yin Y, Qin T, Yang Q (2015) Chicken bone marrow-derived dendritic cells maturation in response to infectious bursal disease virus. Vet Immunol Immunol 164:51–55

    Article  CAS  Google Scholar 

  • Luque D, Rivas G, Alfonso C, Carrascosa JL, Rodríguez JF, Castón JR (2009) Infectious bursal disease virus is an icosahedral polyploid dsRNA virus. PNAS USA 106:2148–2152

    Article  PubMed  PubMed Central  Google Scholar 

  • Mahgoub HA (2012) An overview of infectious bursal disease. Arch Virol 157:2047–2057

    Article  PubMed  CAS  Google Scholar 

  • Martínez-Torrecuadrada JL, Lázaro B, Rodriguez JF, Casal JI (2000) Antigenic properties and diagnostic potential of baculovirus-expressed infectious bursal disease virus proteins VPX and VP3. Clin Diagn Lab Immun 7:645–651

    Google Scholar 

  • Müller H, Mundt E, Eterradossi N, Islam MR (2012) Current status of vaccines against infectious bursal disease. Avian Pathol 41:133–139

    Article  PubMed  Google Scholar 

  • National Research Council (2010) Guide for the Care and Use of Laboratory Animals 8 th ed. National Academies Press, Washington DC

    Google Scholar 

  • Park JH, Sung HW, Yoon BI, Kwon HM (2009) Protection of chicken against very virulent IBDV provided by in ovo priming with DNA vaccine and boosting with killed vaccine and the adjuvant effects of plasmid-encoded chicken interleukin-2 and interferon-γ. J Vet Sci 10:131–139

    Article  PubMed  PubMed Central  Google Scholar 

  • Philbin VJ, Iqbal M, Boyd Y, Goodchild MJ, Beal RK, Bumstead N, Young J, Smith AL (2005) Identification and characterization of a functional, alternatively spliced Toll-like receptor 7 (TLR7) and genomic disruption of TLR8 in chickens. Immunology 114:507–521

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Pitcovski J, Gutter B, Gallili G, Goldway M, Perelman B, Gross G, Krispel S, Barbakov M, Michael A (2003) Development and large-scale use of recombinant VP2 vaccine for the prevention of infectious bursal disease of chickens. Vaccine 21:4736–4743

    Article  PubMed  CAS  Google Scholar 

  • Pradhan SN, Prince PR, Madhumathi J, Roy P, Narayanan RB, Antony U (2012) Protective immune responses of recombinant VP2 subunit antigen of infectious bursal disease virus in chickens. Vet Immunol Immunopathol 148:293–301

    Article  PubMed  CAS  Google Scholar 

  • Rong J, Cheng T, Liu X, Jiang T, Gu H, Zou G (2005) Development of recombinant VP2 vaccine for the prevention of infectious bursal disease of chickens. Vaccine 23:4844–4851

    Article  PubMed  CAS  Google Scholar 

  • Schijns VE, Weining KC, Nuijten P, Rijke EO, Staeheli P (2000) Immunoadjuvant activities of E. coli- and plasmid-expressed recombinant chicken IFN-alpha/beta, IFN-gamma and IL-1beta in 1-day- and 3-week-old chickens. Vaccine 18:2147–2154

    Article  PubMed  CAS  Google Scholar 

  • Sharma JM, Kim I-J, Rautenschlein S, Yeh H-Y (2000) Infectious bursal disease virus of chickens: pathogenesis and immunosuppression. Dev Comp Immunol 24:223–235

    Article  PubMed  CAS  Google Scholar 

  • Swane DE (2008) Diseases of poultry, 13th edn. Wiley-Blackwell, New York

    Google Scholar 

  • Taghavian O, Spiegel H, Hauck R, Hafez HM, Fischer R, Schillberg S (2013) Protective oral vaccination against infectious bursal disease virus using the major viral antigenic protein VP2 produced in Pichia pastoris. PLoS One 8:e83210

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Tovey MG, Lallemand C (2015) Adjuvant activity of cytokines. Methods Mol Biol 2010 626:287–309

    Article  CAS  Google Scholar 

  • Ve T, Williams SJ, Kobe B (2015) Structure and function of Toll/interleukin-1 receptor/resistance protein (TIR) domains. Apoptosis 20:250–261

    Article  PubMed  CAS  Google Scholar 

  • Wu H, Singh N, Locy R, Scissum-Gunn K, Giambrone J (2004) Expression of immunogenic VP2 protein of infectious bursal disease virus in Arabidopsis thaliana. Biotechnol Lett 26:787–792

    Article  PubMed  CAS  Google Scholar 

  • Zanetti FA, Conte Grand MD, Mitarotonda RC, Taboga OA, Calamante G (2014) Canarypox virus expressing infectious bursal disease VP2 protein as immunogen for chickens. Braz J Microbiol 45:231–234

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Funding

This study was supported by the Razi Vaccine and Serum Research Institute [grant no. 2-2-18-93114].

Author information

Authors and Affiliations

  1. Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization, P.O. Box 31975-148, Karaj, Iran

    Mohammad Majid Ebrahimi, Shahla Shahsavandi, Hossein Goudarzi & Shahin Masoudi

  2. Faculty of Veterinary Medicine, Tehran University, P.O. Box 14155-6453, Tehran, Iran

    Mohammad Majid Ebrahimi & Parviz Shayan

Authors
  1. Mohammad Majid Ebrahimi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shahla Shahsavandi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Parviz Shayan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hossein Goudarzi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shahin Masoudi
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Conceived and designed the experiments: S. Shahsavandi. Performed the experiments and analyzed the data: MM. Ebrahimi. Contributed analyzed the data: other co-authors.

Corresponding author

Correspondence to Shahla Shahsavandi.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable National Institutes of Health guide for the care and use of laboratory animals no. 8023 guidelines for the care and use of chickens were followed.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ebrahimi, M.M., Shahsavandi, S., Shayan, P. et al. Recombinant VP2 expressed in baculovirus and adjuvanted with TIR-TLR7: a vaccine candidate against infectious bursal disease virus. Comp Clin Pathol 27, 911–916 (2018). https://doi.org/10.1007/s00580-018-2681-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00580-018-2681-x

Keywords

Search

Navigation