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Pre- and post-partum seric biochemical variables of Lacaune ewes naturally infected by gastrointestinal parasites

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Abstract

The main objective of the present study was to evaluate the biochemical profile of the Lacaune ewes naturally infected by gastrointestinal parasites in the pre- and post-partum period. Ewes with similar age and weight, primiparas in the final stage of gestation, totaling 24 animals were selected. Sampling days were 10 and 2 for pre-partum and 2, 6, and 12 days for post-partum to evaluate total proteins, albumin, globulin, cholesterol, triglycerides, and urea levels, in addition to thiobarbituric acid reactive substances (TBARS), reactive oxygen species (ROS), and glutathione S-transferase (GST). Fecal examination by McMaster method was performed during bleeding, and based on the number of eggs per gram of feces (EPG), the animals were divided into two groups: A (sheep with EPG <1000) and B (sheep with EPG >1000) to evaluate the effect of the parasitic load. Statistical analyses of biochemical parameters and EPG were performed taking into account groups, evaluated each time of sampling and over time, as well as without considering the groups, comparing pre- and post-partum and over time. It was possible to verify that the influence of time had a significant (P < 0.01) impact on cholesterol, triglycerides, total proteins, albumin, globulin, TBARS, and GST specifically among sampling time. When time of collection was considered, there were some differences between groups A and B for the variables: triglycerides (day 6 post-partum) and globulin (day 2 pre-partum). It is concluded that the parasitic load did influence seric levels of the urea, triglycerides, and globulins on a determined time.

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Acknowledgments

The authors thank the farm “Cabanha Chapecó” for providing animals for research.

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Authors and Affiliations

  1. Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Rua Beloni Trombeta Zanin, 680E, Chapecó, 89815-630, Santa Catarina, Brazil

    Alexandre Balzan, Andreia Volpato, Rhayana Grosskopf, Jhonatan P. Boito, Chrystian J. Cazarotto & Aleksandro S. da Silva

  2. Veterinary Medicine Faculty, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Gustavo Machado

  3. Department of Biochemical and Molecular Biology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil

    Nathieli B. Bottari, Maria Rosa C. Schetinger, Vera M. Morsch & Aleksandro S. da Silva

Authors
  1. Alexandre Balzan
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  2. Gustavo Machado
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  3. Nathieli B. Bottari
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  4. Andreia Volpato
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  5. Rhayana Grosskopf
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  6. Jhonatan P. Boito
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  7. Chrystian J. Cazarotto
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  8. Maria Rosa C. Schetinger
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  9. Vera M. Morsch
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  10. Aleksandro S. da Silva
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Corresponding author

Correspondence to Aleksandro S. da Silva.

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Ethics Committee

The experiment was approved by the Ethics Committee on Animal Research of Universidade do Estado de Santa Catarina (UDESC) under protocol number 1.28.15.

Conflict of interest

The authors declare that they have no conflict of interest.

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Balzan, A., Machado, G., Bottari, N.B. et al. Pre- and post-partum seric biochemical variables of Lacaune ewes naturally infected by gastrointestinal parasites. Comp Clin Pathol 25, 815–823 (2016). https://doi.org/10.1007/s00580-016-2268-3

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  • DOI: https://doi.org/10.1007/s00580-016-2268-3

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