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Hematological and serum biochemical alterations in buffalo with some digestive disorders

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Abstract

Hematological and serum biochemical reference intervals are essential laboratory variables for optimal diagnostic purposes. The present study was delineated to provide an insight into the hemato-biochemical alterations as well as the measurement of selected acute phase proteins in buffaloes showing some digestive disorders. The present study included 40 native breed water buffalo showing various symptoms of digestive troubles. Based on competent case history, clinical and laboratory findings, the diseased buffaloes were allocated into four equal sized groups, namely stomatitis, acute traumatic reticuloperitonitis (TRP), acute rumen impaction, and enteritis. Ten apparently healthy buffaloes were randomly selected from the same animal population and considered as control group. Blood samples were drawn from all investigated animals for hematologic examination and measurements of a panel of serum biochemical variables. All diseased buffaloes showed statistically significant high values of total leucocytes (P ≤ 0.05) with significantly low values of total erythrocytes and hemoglobin concentration compared with controls (P ≤ 0.05). Serum magnesium, sodium, and iron showed a statistically (P ≤ 0.05) lower values in all studied buffaloes than those of controls; while serum calcium levels were significantly decreased (P ≤ 0.05) in buffaloes showing stomatitis and those with TRP compared with controls. Serum hepatic enzyme activities beside serum haptoglobin and C-reactive protein were significantly (P ≤ 0.05) higher in all diseased buffaloes than those of controls. The results herein concluded that diseased buffalo were associated with marked hematological and elemental alterations besides a distinct inflammatory reaction. Further studies are needed to explore the potential ameliorative value of using supplementary agents to help mitigate the associated biochemical and inflammatory reactions in buffaloes exhibiting digestive disorders.

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References

  • Baydar E, Dabak M (2014) Serum iron as an indicator of acute inflammation in cattle. J Dairy Sci 97:222–228

    Article  CAS  PubMed  Google Scholar 

  • Bozukluhan K, Gokce H (2007) Investigation of some acute phase proteins in cattle with traumatic reticuloperitonitis or with traumatic pericarditis. J Fac Vet Med Erciyes Univ 4:107–113

    Google Scholar 

  • Ceciliani F, Ceron J, Eckersall PD, Sauerwein H (2012) Acute phase proteins in ruminants. J Proteome 75:4207–4231

    Article  CAS  Google Scholar 

  • El-Ashker M, El-Sebaei M, Fouda M (2012) Evaluation of the inflammatory reaction in calves with acute ruminal drinking. J Vet Sci Technol 3:116

    Google Scholar 

  • El-Ashker M, Salama M, El-Boshy M (2013) Traumatic reticuloperitonitis in water buffalo (Bubalus bubalis): clinical findings and the associated inflammatory response. J Vet Med 1-6.

  • El-Ashker M, Salama M, Rizk A, El-Boshy M (2014) The use of inflammatory markers as a prognostic aid for traumatic reticuloperitonitis in water buffalo (Bubalus bubals). Vet Med (Praha) 59:239–246

    Google Scholar 

  • El-Dessouky SA, El-Masry NM (2005) Effect of Cryptosporidium parvum infection on the hematology and blood chemistry of buffalo calves with special reference to the prevalence of infection in adult buffaloes. Assiut Vet Med J 51:108–123

    Google Scholar 

  • Ghanem MM (2010) A comparitive study on traumatic reticuloperitonitis and traumatic pericarditis in Egyptian cattle. Turk J Vet Anim Sci 34:143–153

    CAS  Google Scholar 

  • Ghanem MM, Omnia M, Abdel-Hamid M (2010) Clinical, hematological and biochemical alterations in heat intolerance (panting) syndrome in Egyptian cattle following natural foot and mouth disease virus (FMD). Trop Anim Health Prod 42:1167–1173

    Article  PubMed  Google Scholar 

  • Ghanem M, El-Fkhrany SF, Abd El-Raof Y, El-Attar H (2012) Clinical and haemato-biochemical evaluation of diarrheic neonatal buffalo calves (Bubalas Bubals) with reference to antioxidant changes. Benha Vet Med J 23:275–288

    Google Scholar 

  • Gokce G, Gokce V, Gunes HM, Citil M (2004) Alterations in some hematological biochemical parameters in cattle suffering from foot and mouth disease. Turk J Anim Sci 28:723–727

    Google Scholar 

  • Gozho GN, Krause DO, Plaizier JC (2007) Ruminal lipopolysaccharide concentration and inflammatory response during grain-induced sub acute ruminal acidosis in dairy cows. J Dairy Sci 90:856–866

    Article  CAS  PubMed  Google Scholar 

  • Hirvonen J, PyӦrӓlӓ S (1998) Acute-phase response in dairy cows with surgically treated abdominal disorders. Vet J 155:53–61

    Article  CAS  PubMed  Google Scholar 

  • Jafarzadeh SR, Nowrouzian I, Khak Z, Ghamsari SM, Adibhashemi F (2004) The sensitivities and specificities of total plasma protein and plasma fibrinogen for the diagnosis of traumatic reticuloperitonitis in cattle. Prev Vet Med 65(1–2):1–7

    Article  PubMed  Google Scholar 

  • Kaneko JJ, Harvey JW Bruss ML (2008) Clinical biochemistry of domestic animals. 6th ed. California, USA, Academic Press.

  • Khafipour E, Krause DO, Plaizier JC (2009) A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation. J Dairy Sci 92:1060–1070

    Article  CAS  PubMed  Google Scholar 

  • Kirbas A, Ozkanlar Y, Aktas MS, Ozkanlar S, Ulas N, Erol HS (2015) Acute phase biomarkers for inflammatory response in dairy cows with traumatic reticuloperitonitis. Israel J Vet Med 70:23–30

    Google Scholar 

  • Lal SB, Dwivedi SK, Sharma MC, Swarup D (1992) Biopathological studies in experimentally induced ruminal acidosis in goats. Indian J Anim Sci 62:200–204

    CAS  Google Scholar 

  • Mohan GC, Kumar AC, Naik BR (2015) Effect of rumen fermentative disorders on physiological parameters in buffaloes. Inter J Vet Sci 4:10–14

    Google Scholar 

  • Mohapatra AK, Kundu AK, Bisoi PC, Prusty BM (2005) Hematological and biochemical changes in crossbred cattle affected with foot and mouth disease. Indian Vet J 82:141–144

    CAS  Google Scholar 

  • Murata H, Miyamoto T (1993) Bovine haptoglobin as a possible immunomodulator in the sera of transported calves. Br Vet J 149:277–283

    Article  CAS  PubMed  Google Scholar 

  • Neamat-Allah A (2015) Alterations in some hematological and biochemical parameters in Egyptian buffaloes suffering from traumatic reticuloperitonitis and its sequelae. Bulletin UASVM Vet Med 72(1):117–122

    CAS  Google Scholar 

  • Nikolov Y (1998) Clinical experimental studies on acute rumen acidosis in buffaloes (Bubalus bubalis), IV. Influence of acidosis on blood, rumen liquid and urine electrolytes. Vet Arh 68(1):1–9

    CAS  Google Scholar 

  • Petersen HH, Nielsen JP, Heegaard PH (2004) Application of acute-phase protein measurements in veterinary clinical chemistry. Vet Res 35:163–187

    Article  CAS  PubMed  Google Scholar 

  • Radostits OM, Doreen HG, Houston M (2000) Veterinary clinical examination and diagnosis. WB Saunders, Elsevier Health Sciences, ​St. Louis, USA

  • Radostits OM, Gay CC, Hinchcliff KW, Constable PD (2007) Veterinary medicine: a textbook of the diseases of cattle, sheep, goats, pigs, and horses, 10th edn. WB Saunders Company Ltd., Philadelphia, Pennsylvania

    Google Scholar 

  • Ulutas B, Tan T, Ulutas PA, Bayramli G (2011) Haptoglobin and serum amyloid responses in cattle persistently infected with bovine viral diarrhea virus. Acta Sci Vet 39:973

    Google Scholar 

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Authors and Affiliations

  1. Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt

    Mohamed A. Youssef & Maged R. El-Ashker

  2. Animal Health Research Institute-Mansoura Provincial Laboratory, Mansoura, Egypt

    Mohamed F. Ouda

Authors
  1. Mohamed A. Youssef
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  2. Maged R. El-Ashker
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  3. Mohamed F. Ouda
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Contributions

MAY and MRE designed and coordinated the study. They were also responsible for data collection, analyses , and interpretation. MRE responsible for writing and reviewing of the manuscript and corresponded with the journal. MFO responsible for clinical examinations, participated in samples collection, and took part in writing of the manuscript. All authors approved the final version of the manuscript for publication.

Corresponding author

Correspondence to Maged R. El-Ashker.

Ethics declarations

All procedures performed were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The farm owners were asked to sign consent for agreeing to the proposed testing and were given a document contains information about the disease and the potential clinical consequences.

Conflict of interest

The authors declare that they have no conflict of interest.

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Youssef, M.A., El-Ashker, M.R. & Ouda, M.F. Hematological and serum biochemical alterations in buffalo with some digestive disorders. Comp Clin Pathol 26, 1033–1039 (2017). https://doi.org/10.1007/s00580-017-2480-9

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  • DOI: https://doi.org/10.1007/s00580-017-2480-9

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