Abstract
The object of this study was in vivo evaluation of the effects of some common antibiotics in treatment of microbial infections on neutrophil phagocytosis in sheep. A period of antibiotic therapy administered in six sheep female lambs (oxytetracycline 5 mg/kg body weight (BW), enrofloxacin 5 mg/kg BW, penicillin 20,000 IU/kg BW, and lincospectin 1 ml/10 kg BW). Blood sample was taken in first, third, sixth, and ninth day of antibiotic therapy (first sample in day 1 was prior to administration) and tested for yeast phagocytosis. Mixed model (REML) was used as a statistical procedure. The results of this study show that estimated mean percentages of yeast phagocytosis were statistically significant between all antibiotics (P < 0.0001). Generally, antibiotics enhanced yeast phagocytosis up to sixth day. Lincospectin and oxytetracycline values returned in ninth day to its previous state on the first day, but, enrofloxacin and penicillin values were high even in ninth day (i.e., they need more time to decrease to the levels before drug administration). In conclusion, in vivo antibiotic therapy enhanced neutrophil in vitro yeast phagocytosis test.
Similar content being viewed by others
References
Braga PC, Dal Sasso M, Bovio C, Zavaroni E, Fonti E (2002) Effects of gatifloxacin on phagocytosis, intracellular killing and oxidant radical production by human polymorphonuclear neutrophils. Int J Antimicrob Agents 19:183–187
Brousseau P, Payette Y, Tryphonas H (1999) Manual of immunological methods, 1st edn. CRC Press, London, pp 20–45
Emami J (2006) In vitro–in vivo correlation: from theory to applications. J Pharm Pharm Sci 9(2):169–189
Feldman BF, Zinkl JG, Jain NC (2000) Schalm’s veterinary hematology, 5th edn. Williams & Wilkins, Canada
Gooi HG, Chapel H (1990) Clinical immunology (a practical approach), 1st edn. Oxford University Press, London, pp 51–80
Gorrini M, Lupi A, Viglio S, Pamparana F, Cetta G, Iadarola P, Powers JC, Luisetti M (2001) Inhibition of human neutrophil elastase by erythromycin and flurythromycin, two macrolide antibiotics. Am J Respir Cell Mol Biol 25:492–499
Gürer US, Göçer P, Erçağ E, Erten N, Rayaman E, Gürbüz B, Üzer A, Karan A, Çevikbaş A (2006) The effects of some antibiotics on polymorphonuclear leukocyte functions of elderly patients in vitro before and after zinc supplementation. Int Immunopharmacol 6:808–816
Hoeben D, Burvenich C, Heyneman R (1998) Antibiotics commonly used to treat mastitis and respiratory burst of bovine polymorphonuclear leukocytes. J Dairy Sci 81:403–410
Paape MJ, Miller RH, Ziv G (1990a) Effects of florfenicol, chloramphenicol, and thiamphenicol on phagocytosis, chemiluminescence, and morphology of bovine polymorphonuclear neutrophil leukocytes. J Dairy Sci 73:1734–1744
Paape MJ, Nickerson SC, Ziv G (1990b) In vivo effects of chloramphenicol, tetracycline, gentamycin on bovine neutrophil function and morphologic features. Am J Vet Res 51:1055–1061
Ritts RE (1990) Antibiotics as biological response modifiers. J Antimicrob Chemother 26(Suppl C):31–36. doi:10.1093/jac/26.suppl_C.31
Roques C, Frayret MN, Luc J, Michel G, Perruchet AM, Cauquil J, Levy D (1991) Effect of an in vivo immunostimulant treatment on PMN functions: interaction with antibiotics in vitro. Int J Immunopharmacol 13(8):1051–1057
Serezli R, Çağirgan H, Okumuş I, Akhan S, Balta F (2005) The effect of oxytetracycline on non-specific immune response in sea bream (Sparus aurata L. 1758). Turk J Vet Anim Sci 29:31–35
Voiculescu C, Stanciu L, Voiculescu M, Rogoz S, Dumitriu I (1983) Experimental study of antibiotic-induced immunosuppression in mice. I. Humoral and cell-mediated immune responsiveness related to in vivo antibiotic treatment. Comp Immunol Microbiol Infect Dis 6(4):291–299
Acknowledgments
This work was supported by a grant from the Islamic Azad University, Tabriz branch (Iran), and by an endowment from Animal Science Department of Agriculture Faculty.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rezapour, A. The effects of several antibiotics on neutrophil phagocytosis in the peripheral blood of sheep (in vivo). Comp Clin Pathol 23, 29–31 (2014). https://doi.org/10.1007/s00580-012-1564-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00580-012-1564-9