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The large (134.9 kb) mitochondrial genome of the glomeromycete Funneliformis mosseae

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Abstract

Funneliformis mosseae is among the most ecologically and economically important glomeromycete species and occurs both in natural and disturbed areas in a wide range of habitats and climates. In this study, we report the sequencing of the complete mitochondrial (mt) genome of F. mosseae isolate FL299 using 454 pyrosequencing and Illumina HiSeq technologies. This mt genome is a full-length circular chromosome of 134,925 bp, placing it among the largest mitochondrial DNAs (mtDNAs) in the fungal kingdom. A comparative analysis with publically available arbuscular mycorrhizal fungal mtDNAs revealed that the mtDNA of F. mosseae FL299 contained a very large number of insertions contributing to its expansion. The gene synteny was completely reshuffled compared to previously published glomeromycotan mtDNAs and several genes were oriented in an anti-sense direction. Furthermore, the presence of different types of introns and insertions in rnl (14 introns) made this gene very distinctive in Glomeromycota. The presence of alternative genetic codes in both initiation (GUG) and termination (UGA) codons was another new feature in this mtDNA compared to previously published glomeromycotan mt genomes. The phylogenetic analysis inferred from the analysis of 14 protein mt genes confirmed the position of the Glomeromycota clade as a sister group of Mortierellomycotina. This mt genome is the largest observed so far in Glomeromycota and the first mt genome within the Funneliformis clade, providing new opportunities to better understand their evolution and to develop molecular markers.

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Acknowledgments

Support for this work came from the Natural Sciences and Engineering Research Council of Canada (NSERC) and Premier Tech, which are greatly acknowledged. We thank Dr. Joe Morton for providing F. mosseae FL299 material, and Drs. Yves Terrat, Denis Beaudet and B. Franz Lang for assistance in bioinformatics. We also thank Dr. David Morse, Dr. Dave Janos, and two anonymous reviewers for the helpful comments and English editing.

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  1. Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, 4101 Rue Sherbrooke Est, Montréal, QC, H1X 2B2, Canada

    Maryam Nadimi, Franck O. P. Stefani & Mohamed Hijri

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  1. Maryam Nadimi
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  2. Franck O. P. Stefani
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  3. Mohamed Hijri
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Correspondence to Mohamed Hijri.

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Nadimi, M., Stefani, F.O.P. & Hijri, M. The large (134.9 kb) mitochondrial genome of the glomeromycete Funneliformis mosseae . Mycorrhiza 26, 747–755 (2016). https://doi.org/10.1007/s00572-016-0710-7

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