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Pathway and sink activity for photosynthate translocation in Pisolithus extraradical mycelium of ectomycorrhizal Pinus thunbergii seedlings

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Abstract

The purpose of this study was to identify the pathway and sink activity of photosynthate translocation in the extraradical mycelium (ERM) of a Pisolithus isolate. We labelled ectomycorrhizal (ECM) Pinus thunbergii seedlings with 14CO2 and followed 14C distribution within the ERM by autoradiography. 14C photosynthate translocation in the ERM resulted in 14C distribution in rhizomorphs throughout the ERM, with 14C accumulation at the front. When most radial mycelial connections between ECM root tips and the ERM front were cut, the whole allocation of 14C photosynthates to the ERM was reduced. However, the overall pattern of 14C distribution in the ERM was maintained even in regions immediately above and below the cut, with no local 14C depletion or accumulation. We inferred from this result that every portion in the ERM has a significant sink activity and a definite sink capacity for photosynthates and that photosynthates detour the cut and reach throughout the ERM by translocation in every direction. Next, we prepared paired ECM seedlings, ERMs of which had been connected with each other by hyphal fusion, alongside, labelled the left seedling with 14CO2, and shaded none, one or both of them. 14C photosynthates were acropetally and basipetally translocated from the left ERM to ECM root tips of the right seedling through rhizomorphs in the left and right ERMs, respectively. With the left seedling illuminated, 14C translocation from the left to the right ERM increased by shading the right seedling. This result suggests that reduced photosynthate transfer from the host to its ERM increased sink activity of the ERM.

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Acknowledgments

This work was supported in part by Grants-in-Aid for Scientific Research (No. 21248018 and No. 23380080) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan

    Munemasa Teramoto, Bingyun Wu & Taizo Hogetsu

  2. Present address: Center for Global Environmental Research, National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, 305-8506, Japan

    Munemasa Teramoto

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  1. Munemasa Teramoto
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Correspondence to Munemasa Teramoto.

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Teramoto, M., Wu, B. & Hogetsu, T. Pathway and sink activity for photosynthate translocation in Pisolithus extraradical mycelium of ectomycorrhizal Pinus thunbergii seedlings. Mycorrhiza 26, 453–464 (2016). https://doi.org/10.1007/s00572-016-0684-5

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