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The ectomycorrhizas of Lactarius cuspidoaurantiacus and Lactarius herrerae associated with Alnus acuminata in Central Mexico

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Abstract

Two pure Alnus acuminata stands established in a montane forest in central Mexico (Puebla State) were monitored between 2010 and 2013 to confirm and recognize the ectomycorrhizal (EcM) systems of A. acuminata with Lactarius cuspidoaurantiacus and Lactarius herrerae, two recently described species. Through comparison of internal transcribed spacer (ITS) of nuclear ribosomal DNA sequences from basidiomes and ectomycorrhizas sampled in the forest stands, we confirmed their ectomycorrhizal association. The phytobiont was corroborated by comparing ITS sequences obtained from EcM root tips and leaves collected in the study site and from other sequences of A. acuminata available in Genbank. Detailed morphological and anatomical descriptions of the ectomycorrhizal systems are presented and complemented with photographs.

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Acknowledgments

We appreciate the assistance in the field and in the laboratory by Bióls. D. Ramos and J.C. Corona (both at Instituto de Ecología, A.C.). Also, we recognize the help of Dr. A. Ramos (postdoctoral at Bandala and Montoya Lab) and Dra. B. Pérez Hernández (at Presequencing Laboratory, Biodiversity and Systematics Net, Instituto de Ecología, A.C.) for their aid in some molecular procedures. We are especially indebted to Dr. E. Ruíz for the important suggestions during the molecular procedures and to Dr. S. Zamudio, for his valuable help in the taxonomic identification of the Alnus tree (both from Instituto de Ecología, A.C., Centro Regional del Bajío). We thank Dr. R. Molina and referees for the careful revision of the manuscript and for their constructive suggestions that enhanced the revised version. We recognize the support given by CONACYT (225382) to the Pre-Sequencing Laboratory, net Biodiversidad and Systematics, INECOL, which facilitated our work during the molecular procedures.

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  1. Biodiversidad y Sistemática, Instituto de Ecología, A.C., P.O. Box 63, Xalapa, Veracruz, 91000, México

    Leticia Montoya, Victor M. Bandala & Edith Garay-Serrano

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  1. Leticia Montoya
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  2. Victor M. Bandala
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  3. Edith Garay-Serrano
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Correspondence to Leticia Montoya.

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Montoya, L., Bandala, V.M. & Garay-Serrano, E. The ectomycorrhizas of Lactarius cuspidoaurantiacus and Lactarius herrerae associated with Alnus acuminata in Central Mexico. Mycorrhiza 25, 457–467 (2015). https://doi.org/10.1007/s00572-015-0625-8

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