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The influence of phosphorus availability and Laccaria bicolor symbiosis on phosphate acquisition, antioxidant enzyme activity, and rhizospheric carbon flux in Populus tremuloides

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Abstract

Many forest tree species are dependent on their symbiotic interaction with ectomycorrhizal (ECM) fungi for phosphorus (P) uptake from forest soils where P availability is often limited. The ECM fungal association benefits the host plant under P limitation through enhanced soil exploration and increased P acquisition by mycorrhizas. To study the P starvation response (PSR) and its modification by ECM fungi in Populus tremuloides, a comparison was made between nonmycorrhizal (NM) and mycorrhizal with Laccaria bicolor (Myc) seedlings grown under different concentrations of phosphate (Pi) in sand culture. Although differences in growth between NM and Myc plants were small, Myc plants were more effective at acquiring P from low Pi treatments, with significantly lower k m values for root and leaf P accumulation. Pi limitation significantly increased the activity of catalase, ascorbate peroxidase, and guaiacol-dependent peroxidase in leaves and roots to greater extents in NM than Myc P. tremuloides. Phosphoenolpyruvate carboxylase activity also increased in NM plants under P limitation, but was unchanged in Myc plants. Formate, citrate, malonate, lactate, malate, and oxalate and total organic carbon exudation by roots was stimulated by P limitation to a greater extent in NM than Myc plants. Colonization by L. bicolor reduced the solution Pi concentration thresholds where PSR physiological changes occurred, indicating that enhanced Pi acquisition by P. tremuloides colonized by L. bicolor altered host P homeostasis and plant stress responses to P limitation. Understanding these plant–symbiont interactions facilitates the selection of more P-efficient forest trees and strategies for tree plantation production on marginal soils.

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Acknowledgments

We thank Joshua Smith and Nathaniel Chapman for their excellent technical support. The West Virginia University Eberly College of Arts and Sciences and the United States Department of Energy (FG02-06ER64148) provided financial support for this work.

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The authors declare that they have no conflict of interest.

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  1. Department of Biology, West Virginia University, P.O. Box 6057, Morgantown, WV, 26506, USA

    Shalaka Desai, Dhiraj Naik & Jonathan R. Cumming

  2. Biosciences Division, Argonne National Laboratory, Argonne, IL, 60439, USA

    Shalaka Desai

  3. Department of Environmental Sciences, Indian Institute of Advanced Research, Koba Institutional Area, Gandhinagar, Gujarat, 382007, India

    Dhiraj Naik

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Desai, S., Naik, D. & Cumming, J.R. The influence of phosphorus availability and Laccaria bicolor symbiosis on phosphate acquisition, antioxidant enzyme activity, and rhizospheric carbon flux in Populus tremuloides . Mycorrhiza 24, 369–382 (2014). https://doi.org/10.1007/s00572-013-0548-1

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