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Pectin localization in the Mediterranean orchid Limodorum abortivum reveals modulation of the plant interface in response to different mycorrhizal fungi

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Abstract

In most mycorrhizal symbioses, phylogenetically distinct fungi colonize simultaneously the roots of individual host plants. A matter of debate is whether plants can distinguish among these fungal partners and differentiate their cellular responses. We have addressed this question in the orchid mycorrhizal symbiosis, where individual roots of the Mediterranean species Limodorum abortivum can be colonized by a dominant unculturable fungal symbiont belonging to the genus Russula and by more sporadic mycelia in the genus Ceratobasidium (form-genus Rhizoctonia). The phylogenetic position of the Ceratobasidium symbionts was further investigated in this work. Both Russula and Ceratobasidium symbionts form intracellular coils in the cortical roots of L. abortivum, but hyphae are very different in size and morphology, making the two fungi easily distinguishable. We have used John Innes Monoclonal 5, a widely used monoclonal antibody against pectin, to investigate the composition of the symbiotic plant interface around the intracellular coils formed by the two fungal partners. Immunolabelling experiments showed that pectin is exclusively found in the interface formed around the Ceratobasidium, and not around the Russula symbiont. These data indicate that the plant responses towards distinct mycorrhizal fungal partners can vary at a cellular level.

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Acknowledgments

We thank Mara Novero for her technical help. MR was supported by the Fondazione CRT with a Progetto Lagrange fellowship. Research was funded by MIUR (PRIN 2007 Project) and by local funding by the University of Torino.

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Authors and Affiliations

  1. Dipartimento di Biologia vegetale, Università di Torino, V.le Mattioli 25, 10125, Turin, Italy

    Chiara Paduano, Michele Rodda, Enrico Ercole, Mariangela Girlanda & Silvia Perotto

  2. IPP-CNR, V.le Mattioli 25, 10125, Turin, Italy

    Chiara Paduano, Michele Rodda, Enrico Ercole, Mariangela Girlanda & Silvia Perotto

Authors
  1. Chiara Paduano
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  2. Michele Rodda
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  3. Enrico Ercole
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  4. Mariangela Girlanda
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  5. Silvia Perotto
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Correspondence to Silvia Perotto.

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Paduano, C., Rodda, M., Ercole, E. et al. Pectin localization in the Mediterranean orchid Limodorum abortivum reveals modulation of the plant interface in response to different mycorrhizal fungi. Mycorrhiza 21, 97–104 (2011). https://doi.org/10.1007/s00572-010-0315-5

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