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Phylogenetic analysis of Glomeromycota by partial LSU rDNA sequences

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Abstract

We analyzed the large subunit ribosomal RNA (rRNA) gene [LSU ribosomal DNA (rDNA)] as a phylogenetic marker for arbuscular mycorrhizal (AM) fungal taxonomy. Partial LSU rDNA sequences were obtained from ten AM fungal isolates, comprising seven species, with two new primers designed for Glomeromycota LSU rDNA. The sequences, together with 58 sequences available from the databases, represented 31 AM fungal species. Neighbor joining and parsimony analyses were performed with the aim of evaluating the potential of the LSU rDNA for phylogenetic resolution. The resulting trees indicated that Archaeosporaceae are a basal group in Glomeromycota, Acaulosporaceae and Gigasporaceae belong to the same clade, while Glomeraceae are polyphyletic. The results support data obtained with the small subunit (SSU) rRNA gene, demonstrating that the LSU rRNA gene is a useful molecular marker for clarifying taxonomic and phylogenetic relationships in Glomeromycota.

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Acknowledgements

G.A. Silva wishes to acknowledge the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior for a Ph.D. scholarship, and L.C. Maia is in debt to Conselho Nacional de Desenvolvimento Científico e Tecnológico for a research grant. The authors wish to thank J. Morton and M. Saito for providing fungal isolates. We are grateful to Mariangela Girlanda and Stefano Ghignone for the critical reading of the manuscript, as well as the anonymous referees for their text revision. This research was funded by the Italian National Council of Research, Cassa di Risparmio di Torino, and Centro di Eccellenza per la Biosensoristica Vegetale e Microbica.

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Authors and Affiliations

  1. Departamento de Micologia, CCB, Universidade Federal de Pernambuco, Av. Prof. Nelson Chaves, s/n., 50670-420, Recife, PE, Brazil

    Gladstone Alves da Silva & Leonor Costa Maia

  2. Istituto per la Protezione delle Piante (Sezione di Torino) del CNR and Dipartimento di Biologia Vegetale dell’ Università—Viale P.A., Mattioli 25, 10125, Torino, Italy

    Erica Lumini, Paola Bonfante & Valeria Bianciotto

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  1. Gladstone Alves da Silva
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  2. Erica Lumini
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  3. Leonor Costa Maia
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  4. Paola Bonfante
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  5. Valeria Bianciotto
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da Silva, G.A., Lumini, E., Maia, L.C. et al. Phylogenetic analysis of Glomeromycota by partial LSU rDNA sequences. Mycorrhiza 16, 183–189 (2006). https://doi.org/10.1007/s00572-005-0030-9

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  • DOI: https://doi.org/10.1007/s00572-005-0030-9

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