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Coriolis force for facilitating DNA molecular migration and hybridization in compact disk microfluidic platforms

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Abstract

This study investigates the influence of Coriolis force on transport and hybridization of ssDNA molecules in compact disk (CD) microfluidic platform where centrifugal force is used as the driving force. While the effect of Coriolis force on fluid flow in CD microfluidic channels has been studied experimentally and numerically only recently, its influence on ssDNA molecule migration and hybridization has not been investigated so far. This study addresses this phenomenon through numerical simulation and demonstrates that for most practical geometrical configurations and angular velocity ranges reported in the literature, the Coriolis force introduces significant qualitative and quantitative spatial variations in the hybridization of ssDNA molecules, particularly at locations near the periphery. In a particular example investigated here, hybridization was observed to reach steady-state at some locations in about half the time required in the absence of Coriolis force. However, our results further indicate that the time frame for hybridization is so fast (<1 s) that the transient effect due to Coriolis force on the location of hybridization is eventually compensated by the kinetics of hybridization and diffusion-limited reactions that tend to allow a uniform diffusion of target ssDNA over the surface at steady-state. To validate the numerical approach developed here, we carry out several experiments on microfluidic channels in a CD platform. Both numerically and experimentally, Coriolis effect is observed to be significantly influenced by channel width and angular rotations. Our results indicate that for low viscosity fluids, angular velocities as low as 25 rad/s could introduce Coriolis force that is as high as at least 25 % of the main driving centrifugal force.

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References

  • Brenner T (2005) Polymer fabrication and microfluidic unit operations for medical diagnostics on a rotating disk. PhD Thesis, IMTEK, University of Freiburg

  • Brenner T, Zengerle R, Ducrée R (2003) A flow-switch based on Coriolis force. In: Proceedings of the 7th International Conference on Micro Total Analysis Systems (μTAS 2003), Squaw Valley, CA, volume 2 of MESA Monographs. Kluwer Academic, pp 903–906

  • Brenner T, Zengerle R, Ducrée J (2003) A flow-switch based on coriolis force. In: Proceedings of TAS. Kluwer Academic, Lake Tahoe

  • Brenner T, Grumann M, Beer C, Zengerle R, Ducrée J (2003) Microscopic characterization of flow patterns in rotating microchannels. In: Proceedings of MICRO.Tec, Munich

  • Cho YK, Lee JG, Park JM, Lee BS, Lee Y, Ko C (2007) One-step pathogen specific DNA extraction from whole blood on a centrifugal microfluidic device. Lab Chip 5:565–567

    Article  Google Scholar 

  • Das S, Chakraborty S (2007) Transverse electrodes for improved DNA hybridization in microchannels. AIChE J 53(5):1086–1099

    Article  Google Scholar 

  • Das S, Das T, Chakraborty S (2006) Analytical solutions for the rate of DNA hybridization in a microchannel in the presence of pressure-driven and electroosmotic flows. Sens Actuators B Chem 114(2):957–963

    Article  Google Scholar 

  • Ducree J, Brener T, Glatzel T, Zengerle R (2003) Coriolis-induced switching and mixing of laminar flows in rotating microchannels. In: Proceedings of Micro Tech. VDE Verlag GmbH, Berlin, pp 397–404

  • Ducrée J, Brenner T, Haeberle S, Glatzel T, Zengerle R (2005) Patterning of flow and mixing in rotating radial microchannels. Springer, New York

    Google Scholar 

  • Ducrée J, Brenner T, Haeberle S, Glatzel T, Zengerle R (2006) Multilamination of flows in planar networks of rotating microchannels. J Microfluid Nanofluid 2(1):78–84

    Article  Google Scholar 

  • Duffy DC, Gills HL, Lin J, Sheppard NF, Kellogg GJ (1999) Microfabicated centrifugal microfluidic systems: characterization and multiple enzymatic assays. Anal Chem 71(20):4669–4678

    Article  Google Scholar 

  • Erickson D, Li D, Krull UJ (2003) Modelling of DNA hybridization kinetics for spatially resolved biochips. Anal Biochem 317:186–200

    Article  Google Scholar 

  • Haeberle S, Brenner T, Zengerle R, Ducrée J (2006) Centrifugal extraction of plasma from whole blood on a rotating disk. Lab Chip 6:776–781

    Article  Google Scholar 

  • Jia G, MaK-S Kim J, Zoval JV, Peytavi R, Bergeron MG, Madou MJ (2006) Dynamic automated DNA hybridization on a CD (compact disc) fluidic platform. Sens Actuators B Chem 114(1):173–181

    Article  Google Scholar 

  • Kassegne SK, Reese H, Hodko D, Yang JM, Sarkar K, Smolko S, Swanson P, Raymond D, Heller MJ, Madou MJ (2003) Numerical modeling of transport and accumulation of DNA on electronically active biochips. J Sens Actuators B Chem 94:81–98 (Elsevier Science B.V)

    Article  Google Scholar 

  • Kassegne SK, Arya B, Yadav N (2008) Numerical modeling of the effect of histidine protonation on DNA hybridization and pH distribution in electronically active microarrays. Sens Actuators B 143(2010):470–481

    Google Scholar 

  • Kellogg GJ, Arnold TE, Carvalho BL, Duffy DC, Sheppard NF (2000) Centrifugal microfluidics: applications. In: van den Berg A, Olthius W, Bergveld P (eds) Micro total analysis systems 2000. Kluwer Academic Publishers, Amsterdam, pp 239–242

    Chapter  Google Scholar 

  • Kim J, Marafie A, Jia XY, Zoval J, Madou MJ (2006) Characterization of DNA hybridization kinetics in a microfluidic flow channel. Sens Actuators B Chem 113(1):281–289

    Article  Google Scholar 

  • Kim N, Dempsey CM, Zoval J, Sze JY, Madou MJ (2007) Automated microfluidic compact disc (CD) cultivation system of Caenorhabditis elegans. Sens Actuators B Chem 122(2):511–518

    Article  Google Scholar 

  • Kim J, Kido H, Rangel RH, Madou M (2008) Passive flow switching valves on a centrifugal microfluidic platform. Sens Actuators B Chem (Elsevier)

  • Madou MJ, Kellogg GJ (1998) Systems and technologies for clinical diagnostics and drug discovery. In: Cohn GE, Katzir A (eds) The LabCD™, 3259th edn. A centrifuge-based microfluidic platform for diagnosticsSPIE, San Jose, pp 80–93

    Google Scholar 

  • Madou MJ, Lu Y, Lai S, Lee J, Daunert S (2000) A centrifugal microfluidic platform—a comparison. Proc Micro Total Anal Syst Conf 2000:565–570

    Google Scholar 

  • Madou M, Lee L, Daunert S, Lai S, Shih C (2001) Design and fabrication of CD-like microfluidic platforms for diagnostics: microfluidic functions. Biomed Microdevices 3(3):245–254

    Article  Google Scholar 

  • Madou M, Zoval J, Jia G, Kido K, Kim J, Kim N (2006) Lab on a disc. Annu Rev Biomed Eng 8:601–628

    Article  Google Scholar 

  • Riegger L, Grumann M, Nann T, Riegler J, Ehlert O, Mittenbühler K, Urban G, Pastewka L, Brenner T, Zengerle R, Ducrée J (2006) Readout concept for multiplexed bead-based fluorescence immunoassays on centrifugal microfluidic platforms. Sens Actuators A Phys 126:455–462

    Article  Google Scholar 

  • Riegger L, Grumann M, Steigert J, Lutz S et al (2007) Single-step centrifugal hematocrit determination on a 10-$ processing device. Biomed Microdevices 9(6):795–799

    Article  Google Scholar 

  • Rothert A, Millner L, Puckett L, Deo SK, Madou MJ, Daunert S (2005) Whole cell reporter gene-based biosensing systems on a compact disc microfluidics platform. Anal Biochem 342:11–19

    Article  Google Scholar 

  • Zeng J, Banerjee D, Deshpande M, Gilbert JR, Duffy DC, Kellogg GJ (2000) Design analysis of capillary burst valves in centrifugal microfluidics. See Ref. 53, pp 579–82

  • Zhang J, Guo Q, Liu M, Yang J (2008) A lab-on-CD prototype for high-speed blood separation. J Micromech Microeng 18:125025

    Article  Google Scholar 

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Authors and Affiliations

  1. MEMS Research Lab, Department of Mechanical Engineering, College of Engineering, San Diego State University, 5500 Campanile Drive, San Diego, CA, 92182-1323, USA

    Sam Kassegne, Ajit Khosla, Dhruv Patel, Nithesh Paramesh, Nitin Harwood & Bhuvnesh Arya

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  1. Sam Kassegne
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  2. Ajit Khosla
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  3. Dhruv Patel
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  4. Nithesh Paramesh
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  5. Nitin Harwood
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  6. Bhuvnesh Arya
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Correspondence to Sam Kassegne.

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Kassegne, S., Khosla, A., Patel, D. et al. Coriolis force for facilitating DNA molecular migration and hybridization in compact disk microfluidic platforms. Microsyst Technol 21, 719–732 (2015). https://doi.org/10.1007/s00542-014-2087-x

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  • DOI: https://doi.org/10.1007/s00542-014-2087-x

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