Abstract
Rapid switching and measurement of the temperature in a small volume of liquid are of paramount importance for the real-time observation of biomolecular events such as protein folding–unfolding or enzymatic reactions. We present a microthermodevice for high-speed biological measurements that combines a heater for fast temperature shifting (millisecond range) and a Thin Film Thermocouple (TFTC) for accurate temperature measurement during microscopic observation. Device manufacturing as well as its thermal characterization are shown. The device is successfully employed to measure the fluorescent quenching of double-stranded DNA coupled with fluorescent intercalator on millisecond time scale, thus demonstrating its capacity for innovative biological experiments.
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Ando T, Kodera N, Takai E, Maruyama D, Saito K, Toda A (2001) A high-speed atomic force microscope for studying biological macromolecules. PNAS, pp 1247–2468
Arata H, Dupont A, Miné-Hattab J, Disseau L, Renodon-Cornière A, Takahashi M, Viovy JL, Cappello G (2009) Direct observation of twisting steps during Rad51 polymerization on DNA. Proc Natl Acad Sci 106(46):19239–19244
Arata HF, Fujita H (2009) Miniaturized thermocontrol devices enable analysis of biomolecular behavior on their timescales, second to millisecond. Integr Biol 1(5):363–370
Arata HF, Gillot F, Collard D, Fujita H (2009) Millisecond analysis of double stranded dna with fluorescent intercalator by micro-thermocontrol-device. Talanta 79(3):963–966
Arata HF, Gillot F, Nojima T, Fujii T, Fujita H (2008) Millisecond denaturation dynamics of fluorescent proteins revealed by femtoliter container on micro-thermodevices. Lab Chip 8(9):1436–1440
Arata HF, Noji H, Fujita H (2006) Motion control of single f 1 − atpase rotary biomolecular motor using microfabricated local heating devices. Appl Phys Lett 88(8):3902–3903
Arata HF, Rondelez Y, Noji H, Fujita H (2005) Temperature alternation by an on-chip microheater to reveal enzymatic activity of beta-galactosidase at high temperatures. Anal Chem 77(15):4810–4814
Ashkin A, Dziedzic JM, Bjorkholm JE, Chu S (1986) Observation of a single-beam gradient force optical trap for dielectric particles. Opt Lett 11(5):288–290
Belgrader P, Young S, Yuan B, Primeau M, Christel LA, Pourahmadi F, Northrup MA (2001) A battery-powered notebook thermal cycler for rapid multiplex real-time pcr analysis. Anal Chem 73(2):286–289
Bohr MT (2002) Nanotechnology goals and challenges for electronic applications. IEEE Trans Nanotechnol 1(1):56–62
Brewer LR, Bianco PR (2008) Laminar flow cells for single-molecule studies of dna-protein interactions. Nat Methods 5(6):517–525
Craighead H (2006) Future lab-on-a-chip technologies for interrogating individual molecules. Nature 442:387–393
Fumio O, Akira M, Yasuyuki T, Takaoki S, Hiroji O, Mitsuo Y, Tsunetoshi A (2004) 65 nm-node cmos process for low power devices. IEICE 104(249)
Galletto R, Amitani I, Baskin RJ, Kowalczykowski CJ (2006) Direct observation of individual reca filaments assembling on single DNA molecules. Nature 443:875–878
Gao YL, Zhuravlev E, Zou CD, Yang B, Zhai QJ, Schick C (2009) Calorimetric measurements of undercooling in single micron sized snagcu particles in a wide range of cooling rate. Thermochimica Acta 482(1):1–7
Gillot F, Morin FO, Arata HF, Guegan R, Tanaka H, Fujita H (2007) On chip thermal calibration with 8cb liquid crystal of micro-thermal device for biological applications. Lab Chip 7(1):1600–1602
Keem K, Jeong DY, Kim S, Lee MS, Yeo IS, Chung UI, Moon JT (2006) Fabrication and device characterization of omega-shaped-gate zno nanowire field-effect transistors. Nano Lett 6(7):1454–1458
Lagally ET, Medintz I, Mathies RA (2001) Single-molecule DNA amplification and analysis in an integrated microfluidic device. Anal Chem 73(3):565–570
Majumdar A, Lai J, Chandrachood M, Nakabeppu O, Wu Y, Shi Z (1995) Thermal imaging by atomic-force microscopy using thermocouple cantilever probes. Rev Sci Inst 66(6):3584–3592
Minakov AA, Schick C (2007) Ultrafast thermal processing and nanocalorimetry at heating and cooling rates up to 1 mk/s. Rev Sci Inst 78(7):3902–12
Neuman KC, Nagy A (2008) Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy. Nat Methods 5(6):491–505
Sato K, Hibara A, Tokeshi M, Hisamoto H, Kitamori T (2003) Microchip-based chemical and biochemical analysis systems. Adv Drug Deliv Rev 55(3):379–391
Sharma D, MacDonald JC, Iannacchione GS (2006) Thermodynamics of activated phase transitions of 8cb: Dsc and mc calorimetry. J Phys Chem B 110(33):6679–1668
Strick TR, Allemand JF, Bensimon D, Bensimon A, Croquette V (1996) The elasticity of a single supercoiled DNA molecule. Science 271(5257):1835–1837
Svoboda K, Block SM (1994) Biological applications of optical forces. Ann Rev Biophys Biomol Struct 23(1):247–285
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This work was supported by the Japan Society for Promotion of Science.
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Gillot, F., Arata, H.F., Morin, F.O. et al. Manufacturing process and thermal characterization of a fast temperature switching microdevice for real-time biological experiments. Microsyst Technol 16, 1821–1824 (2010). https://doi.org/10.1007/s00542-010-1095-8
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DOI: https://doi.org/10.1007/s00542-010-1095-8