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Expression of intercellular adhesion molecule-1 (ICAM-1) during hypoxia-reoxygenation by sinusoidal endothelial cells (SECs) in an obstructive jaundice model

  • Published:
Journal of Hepato-Biliary-Pancreatic Surgery

Abstract.

Background/Purpose: The aim of the present study was to investigate the expression of intercellular adhesion molecule-1 (ICAM-1) by sinusoidal endothelial cells (SECs) during hypoxia-reoxygenation in an obstructive jaundice model.

Methods: Male Wistar rats aged 6 to 8 weeks were assigned to an obstructive jaundice group (group OJ; n= 8) and a sham-operated control group (group S; n= 8). Using flow cytometry, we determined the percentage of ICAM-1-positive cells and the mean fluorescence intensity (MFI) of ICAM-1 per cell in each group during hypoxia-reoxygenation.

Results: The percentage of ICAM-1-positive cells in group OJ did not change significantly under successive incubation conditions. However, the MFI in group OJ differed significantly between normoxia and 24-h renormoxia. The present study investigated the function of isolated SECs from rats with obstructive jaundice and hypoxia-reoxygenation. The percentage of ICAM-1-positive cells and the MFI of ICAM-1-positive cells showed quite similar changes caused by hypoxia-reoxygenation stress in group S and Group OJ. The ICAM-1 expression increased with hypoxia-reoxygenation in both normal and OJ SECs, and the amount of ICAM-1, on the whole, increased. Obstructive jaundice increased the percentage of ICAM-1-positive cells from the normal state.

Conclusions: The amount of ICAM-1 increased with hypoxia-reoxygenation in obstructive jaundice than normal state.

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Authors and Affiliations

  1. First Department of Surgery, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan, , , , , , JP

    Masakazu Fujii, Shinji Noshima, Chizuru Nakayashiki, Daisuke Hayashi, Toshihiro Inokuchi, Tetsuro Kobayashi & Tadahiko Enoki

Authors
  1. Masakazu Fujii
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  2. Shinji Noshima
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  3. Chizuru Nakayashiki
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  4. Daisuke Hayashi
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  5. Toshihiro Inokuchi
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  6. Tetsuro Kobayashi
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  7. Tadahiko Enoki
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Additional information

Received: July 13, 2001 / Accepted: November 16, 2001

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Fujii, M., Noshima, S., Nakayashiki, C. et al. Expression of intercellular adhesion molecule-1 (ICAM-1) during hypoxia-reoxygenation by sinusoidal endothelial cells (SECs) in an obstructive jaundice model. J Hep Bil Pancr Surg 9, 367–371 (2002). https://doi.org/10.1007/s005340200041

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  • DOI: https://doi.org/10.1007/s005340200041

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