Avoid common mistakes on your manuscript.
Preface
We currently experience a milestone phase in the history of vaccination, with first effective coronavirus disease 2019 (COVID-19) vaccines authorized for human use within only approximately 1 year after the discovery and global expansion of a new human virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Some of these vaccines were produced by established conventional approaches, whereas others reflect breakthroughs of novel technologies that are based on at least three decades of scientific development and have now reached the market of mass vaccination for the first time. In this perspective article, we discuss the specific features of the viral antigen used as an immunogen and present the basic concepts behind current mRNA, adenovector, inactivated whole-virus and subunit vaccines, describing similarities and differences. We also address the structural basis of antigenic variation exhibited by newly emerging viral variants, which may pose new challenges to the immunoprophylaxis of COVID-19 by vaccination.
The viral spike protein
All COVID-19 vaccines that have completed phase 3 clinical trials and are currently applied in different countries of the world use the entire viral spike protein (S) as an immunogen (Figs. 1 and 2). The mode of delivery to the vaccinee and its presentation to the immune system, however, are fundamentally different in these vaccines, as is explained in more detail in the following vaccine-specific sections. The focus on S is based on the fact that antibodies directed to its native conformation are potent inhibitors of virus entry and therefore can neutralize viral infectivity (Fig. 1; [1,2,3]). Importantly, the S protein of coronaviruses is prone to undergo dramatic structural changes that occur at the membrane fusion stage of the viral life cycle and are essential for the infection process (Figs. 1 and 2) (reviewed in [4]). The inherent structural instability of this key antigen has been an important aspect in the design of vaccines, because loss of its native conformation may lead to the induction of antibodies with lower neutralizing potency.
The viral spike is a complex of three identical, membrane-anchored S proteins, which are highly glycosylated and comprise 1273 amino acids each (Fig. 2; [5,6,7]). Recent cryo-electron microscopical analyses of viral particles provide evidence that a relatively low number of spikes (on average only 24) are irregularly distributed in the viral membrane, corresponding to a spike density that is about tenfold lower than that of influenza A virus [8]. Molecular hinges in the membrane-proximal part of S allow tilting in all directions and provide considerable flexibility to the molecule [8, 9].
In the course of natural cellular infection (Fig. 1) (reviewed in more detail in [13]), S is synthesized from one of the viral mRNAs into the lumen of the endoplasmic reticulum (ER) as a membrane-associated protein and transported to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC). The S protein is retained due to an ER retrieval signal and interactions with the viral membrane protein M [14], but some molecules of S are also transported to the plasma membrane [15]. Virus assembly occurs in the ERGIC, and newly formed viral particles are released by exocytosis (reviewed in [15]). In the course of secretion, the S protein is cleaved by the cellular protease furin into the membrane-associated S2 part and the distal S1 part, which however remain associated through non-covalent interactions (Figs. 1 and 2). Both parts provide essential functions during viral entry. S1 mediates binding to ACE2 on target cells through its receptor-binding domain (RBD), which oscillates between an “up” and “down” conformation in the trimeric spike ([5, 6, 16], Fig. 2a,c). Receptor interaction is possible only in the “up” conformation. S2 mediates fusion of the viral membrane with either the plasma membrane or endosomal membranes (Fig. 1), resulting in the release of the RNA genome into the cytoplasm (reviewed in [4]). During this process, S1 falls off and S2 adopts a radically different conformation, the postfusion structure, which is also a trimer (Fig. 2d,e; [12]). Understanding the specific structural properties of the spike protein, especially its built-in instability, is important for the use of S as an immunogen in vaccines.
Untoward effects because of conformational instability of the vaccine antigen have been experienced with a formalin-inactivated vaccine against respiratory syncytial virus (RSV) developed in the 1960s [17]. The vaccine was shown to be responsible for enhanced disease upon natural infection in the following season. This failure was directly related to the fact that the procedure for preparing the vaccine had converted the envelope fusion proteins (responsible for inducing protective neutralizing antibodies) into their postfusion conformation [18]. Therefore, the antibody response was only poorly neutralizing and consisted primarily of antibodies binding to the conformationally flipped protein [19]. This negative experience resulted in elegant, structure-based work that finally led to the stabilization of the protein in its prefusion conformation by the engineering of specific mutations that prevent its undesirable conversion into the postfusion structure [20]. Because of the structural similarities of RSV and coronavirus envelope proteins (both class I fusion proteins, [21]), the same principle of mutational protein stabilization could also be applied to coronavirus S proteins, first to those of SARS‑1 and Middle East respiratory syndrome (MERS) viruses [22, 23] and more recently also to the S protein of SARS-CoV‑2 [5, 6]. Specifically, two consecutive prolines are engineered into a specific site of the S2 moiety of S (Fig. 2d) that prevent the undesirable refolding of S2. Several of the current COVID-19 vaccines make use of this technology for antigen stabilization, as indicated in the specific sections.
Recent studies demonstrated that most neutralizing antibodies (ca. 90%) in human postinfection sera are specific for the RBD [1] and a number of potently neutralizing RBD-specific human monoclonal antibodies have been described in the literature (reviewed in [24]). Despite the apparent immunodominance of the RBD [1], the N‑terminal domain (NTD), which, like the RBD is exposed in the prefusion conformation of the spike (Fig. 2a,c), can also induce extremely potent neutralizing antibodies [25, 26]. Currently, there is no generally accepted in vitro correlate of protection, and the situation is further complicated, because such correlates may differ between different categories of vaccines. The induction of S‑specific virus neutralizing antibodies, however, is considered to be of key importance, although other immune functions (especially those involving specific CD4 and CD8 cells) may contribute to protection [27].
mRNA vaccines
Recently, two COVID-19 mRNA vaccines (from Biontech/Pfizer and Moderna) have been authorized in different countries (including the USA and the EU) after successful clinical trials and are already in widespread use [28, 29]. The two products contain a nucleoside-modified mRNA, encoding the sequence of the full-length S protein with two stabilizing proline mutations in S2 (Figs. 2 and 3a) to preserve the native prefusion conformation. Both vaccines use lipid nanoparticles for delivery. Because of their fragility, these vaccines require storage at −70 °C (Biontech [Biontech, Mainz, Germany]/Pfizer [Pfizer, New York, NY, USA]) or −20 °C (Moderna [Moderna, Cambridge, MA, USA]) and contain 30 µg and 100 µg RNA, respectively. From the data of phase 3 clinical trials, protection rates against disease were as high as 95% and 94.1%, respectively, after 2 vaccinations, with tolerable side reactions [30, 31]. This success represents an enormous leap forward in the establishment of a novel technology at the leading edge of the COVID-19-vaccine landscape.
The concept of using RNA as a form of genetic vaccine is not new and was already developed in the early 1990s, based on the demonstration that in vitro transcribed mRNA is expressed in vivo after direct injection into mouse muscle [32]. The principle appears as elegant as straightforward. An mRNA encoding the vaccine antigen is injected, enters cells and the cellular protein translation machinery produces the appropriate antigen, followed by an effective immune response (Fig. 3). Although the idea caused considerable excitement early on, practical implementation was hampered by a number of technical obstacles, including RNA degradation, inefficient entry into cells and suboptimal translation for producing the antigen in the vaccinee, as well as excessive inflammatory responses caused by the recognition of foreign RNA through receptors of the innate immune system [33]. The delivery and stability problem was solved by the development of lipid-based carriers [34], such as the lipid nanoparticles (LNPs) used in the current RNA vaccines (Fig. 3). The chemical composition of these carriers is documented [28, 29], although the formulation process during production may rely on a number of details representing know-how of the producing companies. At least as important as the application of LNPs for delivery was the modification of the RNA in such a way that innate responses are dampened (to avoid intolerable side reactions and the associated restriction of protein translation), without losing the intrinsic adjuvant activity of RNA and efficiency of antigen synthesis [35,36,37]. These changes are a scientific topic on their own and include the codon optimization of the coding sequence, modifications of untranslated regions both at the 5’ and 3’ ends of the RNA to increase stability and translation efficiency as well as the discovery that introduction of modified nucleotides can significantly contribute to the right balance required for an efficacious and well-tolerated RNA vaccine ([36,37,38]; Fig. 3a). As a consequence of this research, uridines are completely replaced by N1‑methylpseudouridines in both the Biontech/Pfizer and the Moderna vaccines [39, 40]. An mRNA vaccine without uridine replacement and prolonged stability at 4 °C is manufactured by the German company Curevac (Curevac, Tübingen, Germany) [41] and currently undergoes testing in a phase 2b/3 clinical trial [42]. Even without going into the details of the RNA modifications indicated in Fig. 3a, it is apparent that several scientific breakthroughs were required to overcome all of the hurdles associated with RNA delivery and to bring RNA vaccines to the current stage of practical application.
Adenovector vaccines
Like with RNA vaccines, the sudden global need for prophylactic mass immunization against COVID-19 was an enormous driving force for pushing forward previously established adenovector vaccine platforms [43]. Meanwhile, several vaccines based on different non-replicating adenovirus vectors and the full-length S protein have completed phase 3 clinical trials and are used for vaccination campaigns in many countries after approval by national and international authorities. These vaccines include products of the Gamaleya Institute in Moscow (Sputnik V) [44, 45], University of Oxford/AstraZeneca (AstraZeneca, Cambridge, UK) (ChAdOx1-S/AZD1222) [46], the Beijing Institute of Biotechnology/CanSino (CanSino Biologics, Tianjin, China) [47] and Janssen Pharmaceutica (Janssen Pharmaceutica [pharmaceutical company of Johnson & Johnson], Beerse, Belgium) [48].
The idea of using adenoviruses as vectors was originally developed for gene therapy [49] and only later applied to vaccines, building on their capacity to induce potent innate and adaptive immune responses [43, 50]. In the current COVID-19 vaccines and for reasons outlined, different adenoviruses are used as vectors but the basic principle of production platforms and mechanism of action is the same (Fig. 4). The gene for the SARS-CoV‑2 S protein is synthesized as a DNA and engineered into the DNA genome of adenoviruses, replacing an adenovirus gene (E1) that is essential for virus replication (Fig. 4a). Through this manipulation, the adenovirus can no longer replicate and cannot give rise to a full infectious cycle (it is therefore referred to as non-replicating viral vector), but it can still enter cells and express the inserted foreign gene to produce the coronavirus S protein (Fig. 4b). In the course of the single round of infection after vaccination, adenovirus vector particles recognize specific cellular receptors, are internalized by receptor-mediated endocytosis and transported to the nuclear membrane where the viral DNA is transferred into the nucleus. The DNA remains extrachromosomal (like in natural adenovirus infections), but the inserted gene for the S protein is transcribed into a corresponding mRNA that is transported to the cytoplasm, followed by the same sequential steps of S protein production and intracellular transport as in the case of natural infection and mRNA vaccination (compare Figs. 1, 3b and 4b).
For adenovirus-vector vaccines to be effective, high doses of vector particles have to be applied. Upon entry into cells, these particles are recognized by sensors of innate immunity [43, 50] and give rise to the release of cytokines and chemokines that are responsible for side reactions associated with vaccination. This intrinsic reactogenicity has the advantage of a built-in adjuvant effect (similar to that of mRNA vaccines), but at the same time limits the dose that can be used for vaccination. Dose finding was therefore an important aspect of human immunization studies to find the right balance between immunogenicity and reactogenicity. As indicated in the reports of phase 1, 2, and 3 clinical trials, the number of viral particles in the current adenovector vaccines is 5 × 1010 (AstraZeneca [46], Janssen [48]) or 1 × 1011 (Sputnik V [44]). This is a substantial mass of adenovirus antigen contained in the vaccine (representing approximately 9 µg or 18 µg of adenovirus protein; calculation based on approximately 100 million amino acids composing the adenovirus capsid [51]). The amount of adenoviral antigen is thus in the range of the antigen content in conventional inactivated or subunit viral vaccines, resulting in an adenovirus-specific immune response in parallel to that against the S protein in the course of COVID-19 adenovector immunization.
Historically, human adenovirus 5 (hAd5) has pioneered the development of the adenovector platform [43, 50]. This vector is used in the Chinese CanSino vaccine and in one of the two components of Sputnik V [44, 47]. Despite its robust immunogenicity, human populations have substantial seropositivity against adenovirus 5 (e.g. 61% in Europe, 65–100% in Africa; [52]), which can dampen the response to the vaccine antigen as compared to individuals without pre-existing immunity [50, 53]. To avoid this potential disadvantage, other serotypes of adenoviruses have been established as vector platforms [54, 55]. The vaccine manufactured by Janssen (Johnson&Johnson) makes use of human adenovirus 26 (hAd26, global seroprevalence only 5.4–17.8% [52]) and recent results from phase 3 clinical trials indicate an efficacy in preventing moderate and severe COVID-19 at 28 days postvaccination of 72% in the USA, 66% in Latin America, and 57% in South Africa [56]. Based on these data the company has applied for an emergency use authorization in the USA [28]. The same hAd26 vector is also applied as a first dose in the two-component Sputnik V vaccine in combination with adenovirus 5, which is in use in Russia and other countries [57] and has an efficacy of 91.6% in a recently published phase 3 clinical trial [45].
The problem of pre-existing immunity is lowest with the Oxford/AstraZeneca vaccine, because it is based on a chimpanzee adenovirus that has very low seropositivity except for certain parts of Africa [52, 58]. Phase 3 clinical trials have indicated an overall efficacy of 70.4% in preventing COVID-19, and substantial efficacy was already achieved after the first dose [46]. The vaccine has received an emergency use authorization in the UK and was recently licensed in the European Union [29]. Irrespective of pre-existing immunity, however, all adenovector vaccines have to deal with the issue of vaccination-induced vector immunity, which may become a problem for booster vaccinations after several doses [59]. Therefore, the exploitation of prime-boost strategies with different vectors are being investigated, similar to the strategy used in the Sputnik V vaccine (i.e. hAd26 for the first and hAd5 for the second dose) [44, 45].
All adenovector vaccines express the full-length S protein, but only the Janssen hAd26 vaccine contains stabilizing mutations similar to those engineered into the mRNA vaccines [60]. Interestingly, a recent study of HeLa S3 cells transduced with the ChAdOx1 AstraZeneca vaccine vector showed that the S protein is expressed and transported to the plasma membrane in its native prefusion structure, without any stabilizing mutations [61]. These data suggest that the SARS-CoV-2 S protein may be less labile and prone to switch into its postfusion structure as previously assumed. Indications for relative stability in the absence of mutations were also obtained in recent structural studies of a soluble S protein construct lacking the membrane anchor [62].
Inactivated whole-virus vaccines
China has spearheaded the development of conventional inactivated whole-virus vaccines, and so far has achieved considerable success with this traditional and long-established technology. Vaccines produced by the companies Sinovac Biotech (Sinovac, Beijing, China) and Sinopharm (Sinopharm, Beijing, China) have been approved for general public use in China, and the Sinopharm vaccine also in Bahrain and the United Arab Emirates, after clinical trials had shown an efficacy of 86% after 2 vaccinations [63]. Mixed reports were obtained for Sinovac’s vaccine termed Coronavac, ranging from 50.4–78% efficacy in a Brazilian trial, 91.25% in Turkey and 65.3% in Indonesia [64]. Since these data are only figures from press releases it is difficult to assess their significance, and differences may be caused by the application of different criteria for calculating rates of protection. For both vaccines, final published results of phase 3 clinical trials are pending.
The current inactivated vaccines from China as well as a vaccine manufactured by Bharat Biotech (Bharat, Hyderabad, Telangana, India) in India and a vaccine in development by the European company Valneva (Valneva, Saint-Herblain, France) [65] are produced by very similar and established technologies. The virus is grown in Vero cells, chemically inactivated, purified more or less extensively and supplemented with adjuvants. In all cases, inactivation is carried out by betapropiolacton (BPL), which has been successfully used for the preparation of other inactivated vaccines, such as rabies vaccines [66].
Some concern was raised through a recent study that analyzed the molecular architecture of BPL-inactivated SARS-CoV‑2, because it revealed that almost all spikes had adopted a postfusion structure [67] (see section “The viral spike protein”). In contrast, formalin-inactivated virus preparations display the spike protein primarily in its prefusion conformation [8]. Under certain conditions of preparation, BPL may therefore be a suboptimal choice for inactivation of SARS-CoV‑2, because these vaccines could induce a disproportionately high level of binding but non-neutralizing antibodies at the expense of potently neutralizing antibodies. This unresolved issue may be clarified either by structural analyses of the current inactivated vaccines and/or by determining the ratios of binding and neutralizing antibodies in post-vaccination sera in comparison to other vaccines.
Subunit vaccines
The COVID-19 candidate vaccine landscape database compiled by the WHO [65] shows that the category of protein subunit vaccines contributes by far the highest number (32%) to the total of 63 candidates currently in clinical development. Only one of those (NVX-CoV2373, developed and manufactured by US company Novavax (Novavax, Gaithersburg, MD, USA) has so far completed phase 3 clinical trials. As recently reported [68], the vaccine was highly effective in preventing disease (89.3%) in a trial conducted in the UK. Substantially lower protection rates were recorded from a trial in South Africa (60.1%), a discrepancy that might be associated with antigenic differences between the predominantly circulating strains B.1.1.7 (N501Y.V1) in the UK and B.1.351 (N501Y.V2) in South Africa. The possible impact of antigenic changes on vaccine efficacy through mutations in the S protein are specifically addressed in the following section.
The antigen used in the Novavax vaccine is a recombinant full-length S protein with stabilizing mutations [69] produced in Sf9 insect cells. Because the protein in its full-length form (Figs. 1, 3 and 4) is not secreted but associated with cellular membranes, it has to be extracted by detergent solubilization and chromatographically purified in an elaborate production process. Nanoparticles of approximately 40 nm size are formed by mixing the purified protein with Novavax’s proprietary Matrix‑MTM adjuvant, composed of saponin from the tree Quillaja saponaria Molina as well as cholesterol and phospholipid. Detailed structural analyses indicated that the S protein in the vaccine is stably locked in the preferred prefusion conformation and each nanoparticle is studded with up to 14 spike proteins [70]. Because of its stability, the vaccine can be stored at 4 °C for prolonged periods of time and therefore—like adenovector and inactivated vaccines—offers logistic advantages over the currently authorized mRNA vaccines, which require storage at low-freezing temperatures.
Impact of new SARS CoV-2 virus variants on vaccines
Since the appearance and global spread of SARS-CoV‑2, the potential of adaptive mutations was immanent and a concern with respect to vaccine development. Although a plethora of mutations were identified in circulating virus strains over time [71], most of them were unremarkable and the virus appeared to be antigenically stable. Recently, the situation changed through the emergence of virus strains that might be more transmissible but also have mutations at some of the most important antigenic sites of the virus. These variants include the UK strain B.1.1.7 (N501Y.V1), the South African strain B.1.351 (N501Y.V2), and the Brazilian strain B.1.1.28.1 (P.1), which display a varying number of mutations in the S protein (Fig. 5; [71]).
The UK variant has eight mutations in the spike protein, one of them (N501Y) in the immunodominant RBD [1]. At the same exposed site of RBD, the South African variant and the Brazilian variant have two additional mutations (E484K and K417N/K471T, respectively). These two mutations may be especially relevant for antibody recognition, because they cause drastic changes in electrical charges of corresponding epitopes at the protein surface. First data on the comparative neutralization of original and new variants by serum samples obtained after vaccination or infection are consistent with these considerations. The UK mutant was neutralized equally well as the wildtype by postvaccination samples, both after immunization with the Biontech/Pfizer mRNA vaccine [72] and the Moderna mRNA vaccine [73]. In an interim analysis of field efficacy, the Oxford/Astra Zeneca vaccine appeared to be equally protective against B.1.1.7 and canonical lineages, although neutralization titers were ninefold lower [74]. In the Moderna vaccine study [73], substantially reduced neutralization was measured with the South African mutant, although a certain degree of neutralization was still observed with all of the sera from the panel analyzed. Of even more concern are data obtained with the South African mutant and postinfection samples, because not only strong reductions of neutralizing activity but even complete escape from neutralization was observed in several instances with a panel of convalescent plasmas [75]. At the time of this writing, specific data with the Brazilian variant had not yet been published. Based on the nature and location of mutations in S (Fig. 5), however, similar reductions in neutralization can be expected. Recent reports of lower efficacy rates in phase 3 clinical trials of the Novavax subunit vaccine in South Africa than in the UK and the Janssen Adeno26 vaccine in Latin America and South Africa than in the USA (see previous section) may reflect the impact of circulating variant strains on vaccine efficacy. Therefore, close monitoring of the emergence and spread of new variants will be essential not only for the aspect of potentially increased transmissibility but also for the risk of escape from vaccine-induced immunity. Disturbingly, the E484K mutation (characteristic of the South African variant) has recently also been detected in B.1.1.7 isolates from the UK, and independent acquisition events have been suggested [76].
Open questions and future directions
The achievements in developing COVID-19 vaccines are tremendous and unprecedented in the history of medicine. Never have new vaccine technologies been implemented into practical use more rapidly and production capacities for billions of vaccine doses generated so effectively from scratch. A certain degree of excitement if not euphoria is therefore warranted. Nevertheless, not all obstacles may already have been overcome and some questions are pending. One of the most important is of course the issue of antigenic variation, and we are just about to recognize this aspect as a real and not only a theoretical problem. We do not know, however, how prominent it will become and whether a situation of antigenic drift similar to that of influenza virus will develop, requiring continuous update of vaccine compositions. Such adaptations might be most easily achieved with mRNA and vector vaccines. An alternative scenario would be the establishment of a limited number of dominant strains, necessitating the generation of multivalent vaccines. Equally important is the unresolved question of the duration of immunity and whether and at which intervals booster vaccinations will be necessary. Considering the substantially different kinds of vaccines, the question will also arise whether these can be combined in prime-boost schedules and/or exchanged for booster vaccinations. A first clinical study addressing the topic of mixing COVID-19 vaccines has already been launched in the UK [77]. There is an ongoing discussion concerning the question of vaccine-induced herd immunity and whether vaccinated people are still able to transmit the virus on infection, even when they are protected from developing disease. A recently published study on the efficacy of the CHAdOx1 nCoV-19 (AZD1222) vaccine showed that viral burden was strongly reduced after first and second vaccinations, suggesting a potentially strong impact on transmission [46].
After mRNA vaccination, more frequent and more severe side reactions were reported after the second than after the first dose of vaccination [30, 31] and also when given to individuals with a past infection [78], suggesting an effect of specific immunological memory. The mechanisms underlying this phenomenon have not been specifically addressed, but they might be related to the fact that the native S protein as well as processed antigenic peptides in complex with MHCI and/or MHCII molecules are expressed at the surface of mRNA-transduced cells (Fig. 3). Upon booster vaccination, these cells can therefore become a target for cytotoxic immune reactions originating from priming by either vaccination or natural infection [79]. These reactions may involve specific T cells (e.g. tissue-resident memory T cells [80]) and also antibody-mediated mechanisms such as antibody-dependent cellular or complement-mediated cytotoxicity [81]. Why no such increased but rather decreased reactogenicity was observed after the second dose of adenovector vaccination [48, 59], which also results in the expression of viral antigens on target cells (Fig. 4), is currently unknown; however, since mRNA-LNPs are taken up nonspecifically and adenovector particles through specific receptors [43, 82], target cells may differ. This aspect remains to be investigated.
Although an impressive number of COVID-19 vaccines have now been authorized in many countries and used for mass vaccination campaigns, there is still a long list of candidate vaccines in the pipeline. As documented by WHO [65], the number is markedly higher than 200, with 63 in clinical and 174 in preclinical development. Improvements may always be possible, and promising approaches include the development of intranasal vaccines for inducing local immunity [83], self-replicating RNA [33] as well as improved and more thermostable liposome carriers for RNA vaccines [84]. Impressive progress is also made in the area of subunit vaccines that contain only the RBD and use carriers for improving immunogenicity [85,86,87].
Next generation candidates, however, will face the problem of demonstrating vaccine efficacy in a situation where effective vaccines already exist. Placebo controls (as used in the phase 3 trials of the currently authorized vaccines) will be difficult to perform in such a situation, and the demonstration of noninferiority to existing vaccines will require even more participants and make trials even more expensive. A remedy to such obstacles would be the establishment of a reliable in vitro correlate of protection that is accepted by licensing authorities. For the time being, we can be very satisfied with the current progress and confident that emerging problems will be tackled with similar energy and ingenuity as were required for the breakthroughs already achieved.
References
Piccoli L, Park YJ, Tortorici MA, Czudnochowski N, Walls AC, Beltramello M, et al. Mapping neutralizing and Immunodominant sites on the SARS-coV‑2 spike receptor-binding domain by structure-guided high-resolution serology. Cell. 2020;183(4):1024–1042.e2. https://doi.org/10.1016/j.cell.2020.09.037.
Tortorici MA, Beltramello M, Lempp FA, Pinto D, Dang HV, Rosen LE, et al. Ultrapotent human antibodies protect against SARS-CoV‑2 challenge via multiple mechanisms. Science. 2020;370(6519):950. https://doi.org/10.1126/science.abe3354.
Brouwer PJM, Caniels TG, van der Straten K, Snitselaar JL, Aldon Y, Bangaru S, et al. Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability. Science. 2020;369(6504):643. https://doi.org/10.1126/science.abc5902.
Tang T, Bidon M, Jaimes JA, Whittaker GR, Daniel S. Coronavirus membrane fusion mechanism offers a potential target for antiviral development. Antiviral Res. 2020;178:104792. https://doi.org/10.1016/j.antiviral.2020.104792.
Wrapp D, Wang N, Corbett KS, Goldsmith JA, Hsieh C‑L, Abiona O, et al. Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science. 2020;367(6483):1260. https://doi.org/10.1126/science.abb2507.
Walls AC, Park Y‑J, Tortorici MA, Wall A, McGuire AT, Veesler D. Structure, function, and antigenicity of the SARS-coV‑2 spike glycoprotein. Cell. 2020;181(2):281–292.e6. https://doi.org/10.1016/j.cell.2020.02.058.
Yao H, Song Y, Chen Y, Wu N, Xu J, Sun C, et al. Molecular architecture of the SARS-coV‑2 virus. Cell. 2020; https://doi.org/10.1016/j.cell.2020.09.018.
Ke Z, Oton J, Qu K, Cortese M, Zila V, McKeane L, et al. Structures and distributions of SARS-CoV‑2 spike proteins on intact virions. Nature. 2020;588(7838):498–502. https://doi.org/10.1038/s41586-020-2665-2.
Turoňová B, Sikora M, Schürmann C, Hagen WJH, Welsch S, Blanc FEC, et al. In situ structural analysis of SARS-CoV‑2 spike reveals flexibility mediated by three hinges. Science. 2020;370(6513):203. https://doi.org/10.1126/science.abd5223.
Bohne-Lang A, von der Lieth C‑W. GlyProt: in silico glycosylation of proteins. Nucleic Acids Res. 2005;33(Web Server issue):W214–W9. https://doi.org/10.1093/nar/gki385.
Wrobel AG, Benton DJ, Xu P, Roustan C, Martin SR, Rosenthal PB, et al. SARS-CoV‑2 and bat RaTG13 spike glycoprotein structures inform on virus evolution and furin-cleavage effects. Nat Struct Mol Biol. 2020;27(8):763–7. https://doi.org/10.1038/s41594-020-0468-7.
Cai Y, Zhang J, Xiao T, Peng H, Sterling SM, Walsh RM, et al. Distinct conformational states of SARS-CoV‑2 spike protein. Science. 2020;369(6511):1586. https://doi.org/10.1126/science.abd4251.
Heinz FX, Stiasny K. Profile of SARS-CoV‑2. Wien Klin Wochenschr. 2020;132(21):635–44. https://doi.org/10.1007/s00508-020-01763-1.
Boson B, Legros V, Zhou B, Siret E, Mathieu C, Cosset F‑L, et al. The SARS-CoV‑2 envelope and membrane proteins modulate maturation and retention of the spike protein, allowing assembly of virus-like particles. J Biol Chem. 2021;296:100111. https://doi.org/10.1074/jbc.RA120.016175.
Duan L, Zheng Q, Zhang H, Niu Y, Lou Y, Wang H. The SARS-coV‑2 spike glycoprotein biosynthesis, structure, function, and antigenicity: implications for the design of spike-based vaccine Immunogens. Front Immunol. 2020; https://doi.org/10.3389/fimmu.2020.576622.
Benton DJ, Wrobel AG, Xu P, Roustan C, Martin SR, Rosenthal PB, et al. Receptor binding and priming of the spike protein of SARS-CoV‑2 for membrane fusion. Nature. 2020;588(7837):327–30. https://doi.org/10.1038/s41586-020-2772-0.
Kapikian AZ, Mitchell RH, Chanock RM, Shvedoff RA, Stewart CE. An epidemiologic study of altered clinical reactivity to respiratory syncytial (RS) virus infection in children previously vaccinated with an inactivated RS virus vaccine. Am J Epidemiol. 1969;89(4):405–21. https://doi.org/10.1093/oxfordjournals.aje.a120954.
Killikelly AM, Kanekiyo M, Graham BS. Pre-fusion F is absent on the surface of formalin-inactivated respiratory syncytial virus. Sci Rep. 2016;6:34108. https://doi.org/10.1038/srep34108.
Ruckwardt TJ, Morabito KM, Graham BS. Immunological lessons from respiratory syncytial virus vaccine development. Immunity. 2019;51(3):429–42. https://doi.org/10.1016/j.immuni.2019.08.007.
Crank MC, Ruckwardt TJ, Chen M, Morabito KM, Phung E, Costner PJ, et al. A proof of concept for structure-based vaccine design targeting RSV in humans. Science. 2019;365(6452):505. https://doi.org/10.1126/science.aav9033.
Rey FA, Lok SM. Common features of enveloped viruses and implications for immunogen design for next-generation vaccines. Cell. 2018;172(6):1319–34. https://doi.org/10.1016/j.cell.2018.02.054.
Pallesen J, Wang N, Corbett KS, Wrapp D, Kirchdoerfer RN, Turner HL, et al. Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen. Proc Natl Acad Sci. 2017;114(35):E7348. https://doi.org/10.1073/pnas.1707304114.
Kirchdoerfer RN, Wang N, Pallesen J, Wrapp D, Turner HL, Cottrell CA, et al. Stabilized coronavirus spikes are resistant to conformational changes induced by receptor recognition or proteolysis. Sci Rep. 2018;8(1):15701. https://doi.org/10.1038/s41598-018-34171-7.
Jiang S, Zhang X, Yang Y, Hotez PJ, Du L. Neutralizing antibodies for the treatment of COVID-19. Nat Biomed Eng. 2020;4(12):1134–9. https://doi.org/10.1038/s41551-020-00660-2.
McCallum M, Marco AD, Lempp F, Tortorici MA, Pinto D, Walls AC, et al. N‑terminal domain antigenic mapping reveals a site of vulnerability for SARS-CoV‑2. bioRxiv. 2021:2021.01.14.426475. 2021. https://doi.org/10.1101/2021.01.14.426475.
Suryadevara N, Shrihari S, Gilchuk P, VanBlargan LA, Binshtein E, Zost SJ, et al. Neutralizing and protective human monoclonal antibodies recognizing the N‑terminal domain of the SARS-CoV‑2 spike protein. bioRxiv. 2021. https://doi.org/10.1101/2021.01.19.427324.
Vabret N, Britton GJ, Gruber C, Hegde S, Kim J, Kuksin M, et al. Immunology of COVID-19: current state of the science. Immunity. 2020;52(6):910–41. https://doi.org/10.1016/j.immuni.2020.05.002.
https://www.fda.gov/emergency-preparedness-and-response/coronavirus-disease-2019-covid-19/covid-19-vaccines. Accessed 2 Feb 2021.
https://www.ema.europa.eu/en/human-regulatory/overview/public-health-threats/coronavirus-disease-covid-19. Accessed 2 Feb 2021.
Polack FP, Thomas SJ, Kitchin N, Absalon J, Gurtman A, Lockhart S, et al. Safety and efficacy of the BNT162b2 mRNA Covid-19 vaccine. N Engl J Med. 2020;383(27):2603–15. https://doi.org/10.1056/NEJMoa2034577.
Baden LR, El Sahly HM, Essink B, Kotloff K, Frey S, Novak R, et al. Efficacy and safety of the mRNA-1273 SARS-coV‑2 vaccine. N Engl J Med. 2021;384(5):403–16. https://doi.org/10.1056/NEJMoa2035389.
Wolff JA, Malone RW, Williams P, Chong W, Acsadi G, Jani A, et al. Direct gene transfer into mouse muscle in vivo. Science. 1990;247(4949 Pt 1):1465–8. https://doi.org/10.1126/science.1690918.
Pardi N, Hogan MJ, Weissman D. Recent advances in mRNA vaccine technology. Curr Opin Immunol. 2020;65:14–20. https://doi.org/10.1016/j.coi.2020.01.008.
Kowalski PS, Rudra A, Miao L, Anderson DG. Delivering the messenger: advances in technologies for therapeutic mRNA delivery. Mol Ther. 2019;27(4):710–28. https://doi.org/10.1016/j.ymthe.2019.02.012.
Karikó K, Buckstein M, Ni H, Weissman D. Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA. Immunity. 2005;23(2):165–75. https://doi.org/10.1016/j.immuni.2005.06.008.
Linares-Fernández S, Lacroix C, Exposito J‑Y, Verrier B. Tailoring mRNA vaccine to balance innate/adaptive immune response. Trends Mol Med. 2020;26(3):311–23. https://doi.org/10.1016/j.molmed.2019.10.002.
Freund I, Eigenbrod T, Helm M, Dalpke AH. RNA modifications modulate activation of innate toll-like receptors. Genes. 2019; https://doi.org/10.3390/genes10020092.
Jackson NAC, Kester KE, Casimiro D, Gurunathan S, DeRosa F. The promise of mRNA vaccines: a biotech and industrial perspective. Npj Vaccines. 2020;5(1):11. https://doi.org/10.1038/s41541-020-0159-8.
Vogel AB, Kanevsky I, Che Y, Swanson KA, Muik A, Vormehr M, et al. Immunogenic BNT162b vaccines protect rhesus macaques from SARS-CoV‑2. Nature. 2021; https://doi.org/10.1038/s41586-021-03275-y.
Corbett KS, Edwards DK, Leist SR, Abiona OM, Boyoglu-Barnum S, Gillespie RA, et al. SARS-CoV‑2 mRNA vaccine design enabled by prototype pathogen preparedness. Nature. 2020;586(7830):567–71. https://doi.org/10.1038/s41586-020-2622-0.
https://www.curevac.com/covid-19/. Accessed 3 Feb 2021.
https://clinicaltrials.gov/ct2/show/NCT04652102. Accessed 3 Feb 2021.
Coughlan L. Factors which contribute to the immunogenicity of non-replicating adenoviral vectored vaccines. Front Immunol. 2020; https://doi.org/10.3389/fimmu.2020.00909.
Logunov DY, Dolzhikova IV, Zubkova OV, Tukhvatullin AI, Shcheblyakov DV, Dzharullaeva AS, et al. Safety and immunogenicity of an rAd26 and rAd5 vector-based heterologous prime-boost COVID-19 vaccine in two formulations: two open, non-randomised phase 1/2 studies from Russia. Lancet. 2020;396(10255):887–97. https://doi.org/10.1016/S0140-6736(20)31866-3.
Logunov DY, Dolzhikova IV, Shcheblyakov DV, Tukhvatulin AI, Zubkova OV, Dzharullaeva AS, et al. Safety and efficacy of an rAd26 and rAd5 vector-based heterologous prime-boost COVID-19 vaccine: an interim analysis of a randomised controlled phase 3 trial in Russia. Lancet. 2021; https://doi.org/10.1016/S0140-6736(21)00234-8.
Voysey M, Clemens SAC, Madhi SA, Weckx LY, Folegatti PM, Aley PK, et al. Safety and efficacy of the ChAdOx1 nCoV-19 vaccine (AZD1222) against SARS-CoV-2: an interim analysis of four randomised controlled trials in Brazil, South Africa, and the UK. Lancet. 2021;397(10269):99–111. https://doi.org/10.1016/S0140-6736(20)32661-1.
Zhu F‑C, Guan X‑H, Li Y‑H, Huang J‑Y, Jiang T, Hou L‑H, et al. Immunogenicity and safety of a recombinant adenovirus type-5-vectored COVID-19 vaccine in healthy adults aged 18 years or older: a randomised, double-blind, placebo-controlled, phase 2 trial. Lancet. 2020;396(10249):479–88. https://doi.org/10.1016/S0140-6736(20)31605-6.
Sadoff J, Le Gars M, Shukarev G, Heerwegh D, Truyers C, de Groot AM, et al. Interim results of a phase 1–2a trial of Ad26.COV2.S Covid-19 vaccine. N Engl J Med. 2021; https://doi.org/10.1056/NEJMoa2034201.
Wilson JM. Adenoviruses as gene-delivery vehicles. N Engl J Med. 1996;334(18):1185–7. https://doi.org/10.1056/nejm199605023341809.
Lasaro MO, Ertl HC. New insights on adenovirus as vaccine vectors. Mol Ther. 2009;17(8):1333–9. https://doi.org/10.1038/mt.2009.130.
Reddy VS, Natchiar SK, Stewart PL, Nemerow GR. Crystal structure of human adenovirus at 3.5 A resolution. Science. 2010;329(5995):1071–5. https://doi.org/10.1126/science.1187292.
Tumban E. Lead SARS-CoV‑2 candidate vaccines: expectations from phase III trials and recommendations post-vaccine approval. Viruses. 2021;13(1):54.
Fausther-Bovendo H, Kobinger GP. Pre-existing immunity against Ad vectors: humoral, cellular, and innate response, what’s important? Hum Vaccin Immunother. 2014;10(10):2875–84. https://doi.org/10.4161/hv.29594.
Morris SJ, Sebastian S, Spencer AJ, Gilbert SC. Simian adenoviruses as vaccine vectors. Future Virol. 2016;11(9):649–59. https://doi.org/10.2217/fvl-2016-0070.
Custers J, Kim D, Leyssen M, Gurwith M, Tomaka F, Robertson J, et al. Vaccines based on replication incompetent Ad26 viral vectors: Standardized template with key considerations for a risk/benefit assessment. Vaccine. 2020; https://doi.org/10.1016/j.vaccine.2020.09.018.
Ledford H. J&J’s one-shot COVID vaccine offers hope for faster protection. Nature. 2021; https://doi.org/10.1038/d41586-021-00119-7.
https://sputnikvaccine.com. Accessed 3 Feb 2021.
Dicks MDJ, Spencer AJ, Edwards NJ, Wadell G, Bojang K, Gilbert SC, et al. A novel chimpanzee adenovirus vector with low human seroprevalence: improved systems for vector derivation and comparative Immunogenicity. Plos One. 2012;7(7):e40385. https://doi.org/10.1371/journal.pone.0040385.
Ramasamy MN, Minassian AM, Ewer KJ, Flaxman AL, Folegatti PM, Owens DR, et al. Safety and immunogenicity of ChAdOx1 nCoV-19 vaccine administered in a prime-boost regimen in young and old adults (COV002): a single-blind, randomised, controlled, phase 2/3 trial. Lancet. 2020;396(10267):1979–93. https://doi.org/10.1016/S0140-6736(20)32466-1.
Mercado NB, Zahn R, Wegmann F, Loos C, Chandrashekar A, Yu J, et al. Single-shot Ad26 vaccine protects against SARS-CoV‑2 in rhesus macaques. Nature. 2020;586(7830):583–8. https://doi.org/10.1038/s41586-020-2607-z.
Watanabe Y, Mendonça L, Allen ER, Howe A, Lee M, Allen JD, et al. Native-like SARS-CoV‑2 spike glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine. bioRxiv. 2021:2021.01.15.426463.. 2021. https://doi.org/10.1101/2021.01.15.426463.
Gobeil SMC, Janowska K, McDowell S, Mansouri K, Parks R, Manne K, et al. D614G mutation alters SARS-coV‑2 spike conformation and enhances protease cleavage at the S1/S2 junction. Cell Rep. 2021;34(2):108630. https://doi.org/10.1016/j.celrep.2020.108630.
Forni G, Mantovani A, Forni G, Mantovani A, Moretta L, Rappuoli R, et al. COVID-19 vaccines: where we stand and challenges ahead. Cell Death Differ. 2021;28(2):626–39. https://doi.org/10.1038/s41418-020-00720-9.
Mallapaty S. China COVID vaccine reports mixed results—what does that mean for the pandemic? Nature. 2021; https://doi.org/10.1038/d41586-021-00094-z.
https://www.who.int/publications/m/item/draft-landscape-of-covid-19-candidate-vaccines. Accessed 2 Feb 2021.
Rupprecht CE, Nagarajan T, Ertl H. Rabies vaccines. In: Orenstein WA, Offit PA, Edwards KM, editors. Plotkin’s vaccines. 7th ed. : Elsevier; 2018. pp. 918–942.e12.
Liu C, Mendonça L, Yang Y, Gao Y, Shen C, Liu J, et al. The architecture of inactivated SARS-coV‑2 with postfusion spikes revealed by Cryo-EM and Cryo-ET. Structure. 2020;28(11):1218–1224.e4. https://doi.org/10.1016/j.str.2020.10.001.
Wadman M, Cohen J. Novavax vaccine delivers 89 % efficacy against COVID-19 in U.K.—but is less potent in South Africa. Science. 2021; https://doi.org/10.1126/science.abg8101.
Tian J‑H, Patel N, Haupt R, Zhou H, Weston S, Hammond H, et al. SARS-CoV‑2 spike glycoprotein vaccine candidate NVX-CoV2373 immunogenicity in baboons and protection in mice. Nat Commun. 2021;12(1):372. https://doi.org/10.1038/s41467-020-20653-8.
Bangaru S, Ozorowski G, Turner HL, Antanasijevic A, Huang D, Wang X, et al. Structural analysis of full-length SARS-CoV‑2 spike protein from an advanced vaccine candidate. Science. 2020;370(6520):1089. https://doi.org/10.1126/science.abe1502.
https://cov-lineages.org/index.html. Accessed 2 Feb 2021.
Muik A, Wallisch AK, Sänger B, Swanson KA, Mühl J, Chen W, et al. Neutralization of SARS-CoV‑2 lineage B.1.1.7 pseudovirus by BNT162b2 vaccine-elicited human sera. Science. 2021; https://doi.org/10.1126/science.abg6105.
Wu K, Werner AP, Moliva JI, Koch M, Choi A, Stewart-Jones GBE, et al. mRNA-1273 vaccine induces neutralizing antibodies against spike mutants from global SARS-CoV‑2 variants. bioRxiv. 2021 Jan 25;2021.01.25.427948. https://doi.org/10.1101/2021.01.25.427948. (Preprint).
Emary KR, Golubchik T, Aley PK, Ariani CV, Angus BJ, Bibi S, et al. Efficacy of ChAdOx1 nCoV-19 (AZD1222) vaccine against SARS-CoV‑2 VOC 202012/01 (B. 1.1. 7). 2021. https://doi.org/10.2139/ssrn.3779160.
Wibmer CK, Ayres F, Hermanus T, Madzivhandila M, Kgagudi P, Lambson BE, et al. SARS-CoV‑2 501Y.V2 escapes neutralization by South African COVID-19 donor plasma. bioRxiv. 2021:2021.01.18.427166. 2021. https://doi.org/10.1101/2021.01.18.427166.
Technical briefing 5.. https://www.gov.uk/government/publications/investigation-of-novel-sars-cov-2-variant-variant-of-concern-20201201. Accessed 4 Feb 2021.
Ledford H. Could mixing COVID vaccines boost immune response? Nature. 2021; https://doi.org/10.1038/d41586-021-00315-5.
Krammer F, Srivastava K, Simon V. Robust spike antibody responses and increased reactogenicity in seropositive individuals after a single dose of SARS-CoV‑2 mRNA vaccine. medRxiv. 2021:2021.01.29.21250653. 2021. https://doi.org/10.1101/2021.01.29.21250653.
Igyártó B, Jacobsen S, Ndeupen S. Future considerations for the mRNA–lipid nanoparticle vaccine platform. 2020. https://doi.org/10.20944/preprints202012.0493.v1.
Szabo PA, Miron M, Farber DL. Location, location, location: tissue resident memory T cells in mice and humans. Sci Immunol. 2019;4(34):eaas9673. https://doi.org/10.1126/sciimmunol.aas9673.
Anand SP, Finzi A. Understudied factors influencing Fc-mediated immune responses against viral infections. Vaccines. 2019;7(3):103.
Zeng C, Zhang C, Walker PG, Dong Y. Formulation and delivery technologies for mRNA vaccines. Curr Top Microbiol Immunol. 2020; https://doi.org/10.1007/82_2020_217.
Hassan AO, Kafai NM, Dmitriev IP, Fox JM, Smith BK, Harvey IB, et al. A single-dose intranasal ChAd vaccine protects upper and lower respiratory tracts against SARS-CoV‑2. Cell. 2020;183(1):169–184.e13. https://doi.org/10.1016/j.cell.2020.08.026.
Landhuis E. Seven technologies to watch in 2021. Nature. 2021;589(7843):630–2. https://doi.org/10.1038/d41586-021-00191-z.
Tan TK, Rijal P, Rahikainen R, Keeble AH, Schimanski L, Hussain S, et al. A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV‑2 spike protein receptor-binding domain induces potent neutralising antibody responses. Nat Commun. 2021;12(1):542. https://doi.org/10.1038/s41467-020-20654-7.
Brouwer PJM, Brinkkemper M, Maisonnasse P, Dereuddre-Bosquet N, Grobben M, Claireaux M, et al. Two-component spike nanoparticle vaccine protects macaques from SARS-CoV‑2 infection. Cell. 2021; https://doi.org/10.1016/j.cell.2021.01.035.
Cohen AA, Gnanapragasam PNP, Lee YE, Hoffman PR, Ou S, Kakutani LM, et al. Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice. Science. 2021;371(6530):735. https://doi.org/10.1126/science.abf6840.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
F.X. Heinz and K. Stiasny declare that they have no competing interests.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Heinz, F.X., Stiasny, K. Profiles of current COVID-19 vaccines. Wien Klin Wochenschr 133, 271–283 (2021). https://doi.org/10.1007/s00508-021-01835-w
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00508-021-01835-w