We present a low-cost method to fabricate microfluidic devices with embedded transducers suitable for rapid prototyping on a lab scale and potentially also for mass production. To achieve this goal, a method for fabricating passive microfluidic devices using a solvent bonding technique for sealing is introduced. To avoid clogging, liquid metal (gallium) is utilized as a sacrificial layer during this bonding process. A sensor fabrication method compatible with the rapid prototyping ambitions is screen printing. Sensors based on thermal, capacitive and mechanical effects are fabricated by screen printing and are embedded into channels. The proposed processing methods can be easily adapted to achieve specific requirements associated with different tasks in lab-on-a-chip devices.
In diesem Beitrag wird eine “Low-Cost”-Herstellungsmethode für mikrofluidische Chips mit integrierten Sensoren und Aktoren (Transducer) präsentiert. Aufgrund der Einfachheit des Prozesses ist er speziell für Rapid Prototyping in Forschungs- und Entwicklungslaboren geeignet, potentziell ist er jedoch auch für Massenproduktion anwendbar. Zunächst wird eine Herstellungsmethode für passive mikrofluidische Chips eingeführt, welche ein Lösungsmittelklebeverfahren anwendet, um die Chips zu versiegeln bzw. abzudichten. Um ein Verstopfen der mikrofluidischen Kanäle während dieses Klebevorgangs zu unterbinden, wird flüssiges Metall (Gallium), fungierend als Opferschicht während des Versiegelungsprozesses, in die Kanäle eingefüllt. Als Herstellungsmethode für die integrierten Transducer wird Siebdruck gewählt, da dieser Prozess mit den Rapid-Prototyping-Ambitionen vereinbar ist. Siebgedruckte Sensoren basierend auf thermischen, kapazitiven und mechanischen Effekten werden in mikrofluidische Chips integriert und die gewonnenen Ergebnisse diskutiert. Die vorgestellten Fabrikationsmethoden können leicht auf verschiedenste Anforderungen angepasst und so auf spezifische Aufgaben in Lab-on-a-Chip-Systemen maßgeschneidert werden.
Traditional materials for microfluidic devices are commonly silicon or glass. Fabrication techniques for these materials are usually based on surface and bulk micromachining technologies [1,2,3]. With these fabrication processes, embedding of sensors and actuators for microfluidic tasks is also possible. Mass production of devices is feasible, but until a proper fabrication process is established, the development of suitable technologies is costly .
Over the last few decades, the use of polymeric microfluidic devices was established as a worthwhile alternative to these classic materials and technologies. Due to the wide variety of available polymer materials and processing techniques, combined with the usually affordable costs, they are especially suitable for rapid prototyping on a small scale, but also for mass production [5, 6]. When using appropriate fabrication methods, transducers can be easily embedded into polymeric microfluidic devices. Screen printing technology is one of these suitable methods. Sensors and actuators produced by screen printing technique can be embedded into microchannels without disturbing the flow. Due to a wide variety of readily available screen printing inks, the process is highly flexible. As these inks are usually polymer based, compatibility with the base material can be ensured, resulting in proper adhesion of the printed features to the device.
In this work, we present a concept for integrating sensors and actuators into polymeric devices using a low-cost approach. First, a fabrication method for passive microfluidic devices using gallium as a sacrificial material during a solvent bonding process is presented. Based on this process, several examples for sensor integration, utilizing different physical effects, are discussed. Transduction elements presented here are based on thermal, capacitive and mechanical effects, for measuring quantities like flow velocity or relative permittivity of liquids inside microchannels. The proposed approach is highly flexible and can be easily adapted to other applications.
2 Microchannel fabrication
First, the fundamental fabrication process for passive, polymeric microfluidic devices is discussed . A simple to use process is presented, which eliminates the need for special equipment for bonding (e.g. O2 plasma) by employing gallium as a sacrificial layer during a solvent bonding process. This makes the process especially suitable for rapid prototyping on a small academic lab scale.
The sequence of this fabrication process is shown in Fig. 1. First, the microfluidic channels are structured into a PMMA sheet using a laser engraver (Trotec Speedy 300 flexx, Fig. 1a). Note that also any other method for patterning the channels can be employed, e.g., micromilling or hot embossing. Mechanical threads are cut into the inlet and outlet ports, enabling easy and leakproof connection using threaded Luer adapters (Fig. 1b). As the laser engraving process causes rapid heating and rapid cooling of the polymer, thermal stresses are induced inside the material. If the engraved surface comes then in contact with a solvent, the stresses are relieved, leading to the formation of cracks around the engraved edges. This is commonly referred to as stress crazing [8, 9]. In order to avoid this crack formation during the bonding process, performing an annealing step is recommended [10, 11]. To this end, the engraved piece is placed in a box oven and heated up to approximately 75 ∘C, before allowing it to slowly return to room temperature (Fig. 1c). Next, the engraved piece is clamped channel side down to a glass slide, with a thin polymer film in between (Fig. 1d). This temporarily seals the channel, which makes it possible to fill in pre-heated liquid gallium (Fig. 1e). The workpiece is then cooled in a freezer at −10 ∘C for approximately 5−10 min, in order to solidify the gallium inside the channels (Fig. 1f). To eliminate the risk of supercooling the gallium, a small piece of solid gallium can be immersed in the liquid metal at one of the inlet or outlet ports. This piece of solid then acts as a seeding crystal, accelerating the process of solidification. Since the polymer film between the glass and the channel prevents the gallium from sticking to the glass, the glass slide can be removed subsequently, leaving the engraved channel filled with solid gallium. Next, a liquid solvent suitable to dissolve the channel material (here dichloromethane ) is applied to the top of a flat sheet of PMMA (the bottom plate). Against this surface the engraved workpiece is pressed, with the channel in direct contact with the solvent (Fig. 1g). The solid gallium prevents the channel from being clogged by dissolved polymer, which now seals together the two PMMA parts. Subsequently, the chip is submerged in warm water to liquefy the gallium again, which is then flushed from the channels using a weak sodium hydroxide (NaOH) solution (Fig. 1h). Afterwards, the channels are cleaned using deionized water.
The described fabrication process can be employed to manufacture a variety of passive channel geometries performing different tasks in microfluidic devices, that, for example, can consist of intricate channel features, multiple branches, and three-dimensional structures inside the channel. Figure 2a shows a flow focusing device, which can generate monodisperse emulsions or foams. Deionized (DI) water colored with black ink and a small amount of dishwashing detergent flowed continuously from the vertical channels, whereas air flowed continuously from the horizontal channel. Due to an interplay of viscous forces and interfacial tension, monodisperse air bubbles are generated, where their size and frequency of generation depends on the capillary numbers (ratio of viscous forces to surface tension forces) and the size of the orifice . Figure 2b shows a chemical gradient generator. DI water mixed with blue and yellow ink, respectively, flowed from the inlets on the left side. The two colors were mixed via diffusion in the meander-shaped channels. After the third stage, five different concentrations were available. Creating such defined concentrations can be crucial for cell analysis . The staggered herringbone mixer shown in Fig. 2c features asymmetrical grooves on the channel bottom to create vortices that improve mixing of the two liquids, compared to mixing by purely diffusion . Here, DI water mixed with blue and red ink was mixed over the course of the four mixing stages. The minimum feature sizes of the devices shown here were in lateral direction 160 μm (limited by the laser engraver spot size) and 80 μm in vertical direction.
3 Embedding of transducers
In addition to being fast and low-cost, the beforementioned fabrication technique also allows for the embedding of sensors into microfluidic chips. Following the concept of rapid prototyping based on polymeric materials, screen printing is chosen as a manufacturing technique for processing polymer-based inks. In the following, several examples for screen printed sensors based on different physical effects are presented.
3.1 Thermal effects
One crucial parameter in lab-on-a-chip applications is the velocity of the flow inside microchannels. Widely used methods for monitoring this quantity are based on heat transfer, which can be generally divided into three categories. First, sensors that measure cooling due to the streaming fluid , second, sensors that measure an asymmetry of the temperature profile around a heater caused by the flow , and third, sensors that measure the propagation time of a heat pulse . Thermal mass flow sensors can be operated in different modes, keeping either the applied current (or voltage) constant, or the temperature of heating elements, which is achieved by a control circuit. Using the latter mode, the required power to keep the heating element on the same temperature is a measure for the flow velocity. Keeping the temperature constant eliminates thermal inertia of the sensor, yielding a higher limiting frequency as operation in constant current/voltage mode. Fast fluctuations in flow velocity can therefore be measured in this mode.
Here, we present a flow velocity sensor utilizing the convective heat transfer from a microheater to a temperature sensor [19, 20], which falls into the third category mentioned above. A schematic layout is shown in Fig. 3. The channel geometry consists of one circular chamber with two channels leading from it to two outlets. The sensor structure is designed in a way that a circular heater heats the fluid inside the chamber. A thermocouple is located in each downstream channel to record the temperature of the fluid in the respective channel. When the heater is turned on, heat is transported to the thermocouples via heat conduction and convection. One of the two channels is sealed so that no flow can take place. The temperature difference between the two channels is measured, which is approximately proportional to the flow velocity, as the conductive part of the heat transfer is equal in both channels. Therefore, a relation between the flow velocity and the temperature difference can be established.
The fabrication process of the device is the same as shown in Fig. 1a through 1f. Prior to the solvent bonding process, the described sensor layout is screen printed onto the bottom plate. To do so, first the silver features (heater and one half of each thermocouple) are printed from a polymeric screen printing ink with silver filler particles (KA801 by Dupont) using a 120 threads/cm PET mesh. After this print is dried at 100 ∘C, the other halves of the thermocouples are printed from a carbon black filled polymer ink (EDAG PR 406B by Henkel) in two steps wet-in-wet using a 100 threads/cm PET mesh. Again, the printed structure is dried at 100 ∘C. To protect the printed structures during the solvent bonding process, a layer of PMMA (1:10 solution in anisole) is spin coated on top of it. The resulting PMMA layer thickness is approximately 10 μm. Following this sensor fabrication procedure, the channel fabrication process is resumed with step 1g in Fig. 1. The fabricated microfluidic chip is shown in Fig. 4. The grey and black patterns seen in Fig. 4 are the screen printed silver and carbon black features, respectively.
Measurement results are shown in Fig. 5: The channel was filled with a test fluid (Titan 0W30 oil by Fuchs) and flow was introduced by a syringe pump (ExiGo by Cellix). A power of 380 mW was applied to the heater for 40 seconds and the difference in temperature was recorded for a range of flow velocities (0 to 160 μl/min). Measurements were performed at ambient temperature in the lab. As the thermal mass of the device is assumed to be relatively high, and the heating power relatively low, it is not expected that the device is warmed up significantly during a measurement. Additionally, enough time was given between measurements, to let the device return to ambient temperature. The reference point of the thermocouples was held constant using an aluminum block, whose temperature was controlled using a water bath to be 25 ∘C.
As seen in Fig. 5, the temperature difference increased with increasing flow velocity, which is in agreement with the assumption that this difference originates from the convective heat transfer. Note, that in a technical realization it would be beneficial to add temperature sensors on the device to monitor the ambient temperature, as this is crucial for the reliability of the measurements. Nevertheless, the underlying concept can be demonstrated without this addition.
3.2 Capacitive effects
Capacitive sensors can be used in a variety of applications in lab-on-a-chip devices, e.g., material or droplet detection , monitoring of bacterial growth , the detection of viruses  and sensing of DNA  or chemical processes . Suitable electrode configurations can also be processed by screen printing.
A device for distinguishing fluids with different dielectric properties, which was fabricated using the aforementioned techniques, is shown in Fig. 6. An interdigitated electrode pair on the bottom plate forms a capacitor; the microchannel is placed directly above it. To avoid shorting of the electrodes via a conductive fluid, they are insulated by a thin polymer layer. Depending on the relative permittivity of the fluid inside the channel, the capacitance changes accordingly.
The fabrication process follows the same steps as mentioned above. The interdigitated electrode pair was screen printed (using the same conductive silver ink and screen printing mesh) on the bottom plate, before applying the insulation layer by spin coating process (again from a 10:1 anisole in PMMA solution). Measurement results showing the change in capacitance (compared to the air-filled microchannel) due to liquids with different relative permittivity are shown in Fig. 7 as blue circles.
The change in capacitance depending on the fluid inside the channel is given by
with \(C\)fluid the capacitance related to the fluid inside the channel and \(C\)dielectric the capacitance associated with the dielectric layer . An increase of the relative permittivity of the insulating layer leads to a higher \(C\)dielectric and therefore the total capacitance change depends stronger on the capacitance change caused by the fluid. Therefore, the sensitivity of the device can be increased by improving the dielectric properties of the insulating layer. One way to achieve this is by mixing in particles with a high permittivity, e.g., BaTiO3 (\(\epsilon\)r,BaTiO3 ≈500–6900 ). To this end, the 10:1 anisole in PMMA solution was mixed with BaTiO3 particles. Subsequently, the mixture was milled in a planetary ball mill (for 8 min at 650 rpm) to achieve a homogeneous solution. A ratio of 40 vol% PMMA to 60 vol% BaTiO3 was found to have the highest relative permittivity . The resulting dielectric coating was again applied by spin coating over the interdigitated electrodes. The permittivity of this dielectric was approximately 8 times higher than that of pure PMMA . Therefore, as can be seen in the red triangles in Fig. 7, the sensitivity of the capacitive sensor increased significantly.
3.3 Mechanical effects
Besides thermal effects, also mechanical effects can be employed for measuring the flow velocity inside microchannels [29, 30]. According to Bernoulli’s law, an increase in flow velocity leads to a reduced pressure in the channel. Here, a device is presented, which utilizes a deformable diaphragm for measuring the flow velocity based on the aforementioned effect . If the flow velocity increases, the reduced pressure causes the diaphragm to bend inwards. This deformation is monitored via a screen printed strain gauge on the surface of the diaphragm, which is located atop a circular chamber in the channel (see Fig. 8).
The fabrication process is again based on the process introduced in Sect. 2. However, in order to fabricate the diaphragm, the procedure is modified after step f of Fig. 1. After removing the device from the freezer, a layer of UV curable PMMA is doctor-bladed on top of the gallium filled channel and subsequently crosslinked under UV irradiation for ten minutes. Next, the strain gauge is screen printed onto the surface of the cured PMMA layer in the area of the circular chamber. Here, the sensor is printed using a low temperature curing silver ink (C2121022D1 by Sun Chemical) in order to avoid the risk of damaging the thin UV-cured membrane. The sensor was printed using a 140 threads/cm PET mesh and cured at 75 ∘C for 15 min. The removal of the gallium was performed using the same method as in Sect. 2. The fabricated device is shown in Fig. 9.
Measurement results are shown in Fig. 10. The resistance of the strain gauge was measured using a digital multimeter in 4-wire configuration. As can be seen, the relative change in resistance decreased with increasing flow velocity. Flow velocities above 300 μL/min cannot be distinguished anymore as the diaphragm is physically limited in deformation. Also, flow velocities below 10 μL/min cannot be detected due to the stiffness of the diaphragm. However, the design and thickness of the diaphragm can be adjusted in order to modify the measurement range.
In this work, a concept for integrating transducers into polymeric microfluidic devices was presented. Several possible application examples, based on different physical effects, were discussed. The proposed fabrication concept was found suitable for the low-cost integration of sensors and actuators into lab-on-a-chip applications. As demonstrated, the underlying concept can be easily adapted to meet the varying requirements of different applications in lab-on-a-chip devices. Due to this flexibility, it is especially suitable for rapid prototyping.
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Open access funding provided by Johannes Kepler University Linz. This work has been supported by the COMET-K2 “Center for Symbiotic Mechatronics” of the Linz Center of Mechatronics (LCM) funded by the Austrian federal government and the federal state of Upper Austria.
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Hintermüller, M.A., Offenzeller, C., Knoll, M. et al. Embedded screen-printed transducers in bulk polymer microfluidic devices. Elektrotech. Inftech. 137, 128–134 (2020). https://doi.org/10.1007/s00502-020-00793-x
- microfluidic channel fabrication
- integrated sensors
- flow velocity
- mikrofluidische Kanalherstellung
- integrierte Sensoren