Skip to main content
SpringerLink
Log in

Unilateral and bilateral hybridization barriers in inter-series crosses of 4x 2EBN Solanum stoloniferum, S. pinnatisectum, S. cardiophyllum, and 2x 2EBN S. tuberosum haploids and haploid-species hybrids

  • Original Article
  • Published:
Sexual Plant Reproduction Aims and scope Submit manuscript

Abstract

Wild Mexican potato species are an important untapped source of useful variation for potato improvement. Introgression methods such as 2n gametes, chromosome doubling, and crossing with disomic 4x 2 endosperm balance number (EBN) bridge species have been used to overcome post-zygotic endosperm failure according to the EBN hypothesis. Stylar barriers can prevent zygote formation, bilaterally when zygote formation is blocked in both directions of the cross or unilaterally when zygote formation is blocked in self incompatible (SI) × self compatible (SC) crosses. In several Solanaceae species, the S-locus for SI has been implicated in interspecific incompatibility. The objectives of this research were to determine if: (1) disomic 4x 2EBN Solanum stoloniferum can be used as a bridge species for introgression of the Mexican 2x 1EBN species Solanum cardiophyllum and Solanum pinnatisectum, (2) pre- and/or post-zygotic barriers limit hybridization among EBN compatible Solanum inter-series crosses, and (3) reproductive barriers act unilaterally or bilaterally. Fruit formation and seed set was recorded for inter-pollinations of S. stoloniferum, 4x 2EBN chromosome doubled S. cardiophyllum and S. pinnatisectum, and 2x 2EBN S. tuberosum haploids (HAP) or haploid-species hybrids (H-S). In vivo pollen tube growth was analyzed for each cross combination with fluorescence microscopy. Attempts to create bridge hybrids between S. stoloniferum, and S. cardiophyllum or S. pinnatisectum were not successful. Pre- and post-zygotic barriers prevented seed formation in crosses involving S. cardiophyllum and S. pinnatisectum. Self compatibility in S. stoloniferum and S. pinnatisectum suggests that the S-locus does not contribute to the stylar barriers observed with these species. Alternatively, the presence of functional and nonfunctional (SC) S-alleles may explain interspecific incompatibility in intra- and inter-ploidy crosses. A non-stylar unilateral incongruity was discovered in H-S/HAP × S. stoloniferum crosses, indicating either a post-zygotic barrier, or a pre-zygotic barrier acting at or within the ovary. Furthermore, lack of S. stoloniferum pollen rejection may occur through absence of S. stoloniferum pollen-active genes needed to initiate pollen rejection, or through competitive interaction in S-locus heterozygous S. stoloniferum pollen. Introgression strategies using these species would benefit potato breeding by introducing genetic diversity for several traits simultaneously through co-current introgression.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1a–d
Fig. 2a–h
Fig. 3a–d
Fig. 4a–d

Similar content being viewed by others

References

  • Abdalla MMF, Hermsen JGTh (1972) Unilateral incompatibility: hypothesis, debate and implications for plant breeding. Euphytica 21:37–47

    Google Scholar 

  • Adiwilaga K, Brown CR (1991) Use of 2n pollen-producing triploid hybrids to introduce tetraploid Mexican wild species germplasm to cultivated tetraploid potato gene pool. Theor Appl Genet 81:645–662

    Google Scholar 

  • Bamberg JB, Hanneman RE Jr, Palta JP, Harbage JF (1994) Using disomic 4x(2EBN) potato species’ germplasm via bridge species Solanum commersonii. Genome 37:866–870

    Google Scholar 

  • Budin KZ, Gavrilenko TA (1994) Genetic basis of remote hybridization in potato. Rus J Genet 30:1188–1196

    Google Scholar 

  • Cipar MS, Peloquin SJ, Hougas RW (1964) Inheritance of incompatibility in hybrids between Solanum tuberosum haploids and diploid species. Euphytica 13:163–172

    Google Scholar 

  • Cruz-Garcia F, Hancock CN, McClure B (2003) S-RNase complexes and pollen rejection. J Exp Bot 54:123–130

    Google Scholar 

  • Dinu II, Thill CA (2004) Endosperm and embryo development in interspecific incompatible Solanum crosses (Abstr). Am J Potato Res 81:57

    Google Scholar 

  • Dinu II, Hayes RJ, Kynast RG, Phillips RL, Thill CA (2005) Novel inter-series hybrids in Solanum, section Petota. Theor Appl Genet 110:403–415

    Google Scholar 

  • Eijlander R (1998) Mechanisms of self-incompatibility and unilateral incompatibility in diploid potato (Solanum tuberosum L.). PhD Thesis, Wageningen Agricultural University, Wageningen

  • Hanneman RE Jr (1989) The potato germplasm resource. Am Potato J 66:655–667

    Google Scholar 

  • Hanneman RE Jr (1999) The reproductive biology of the potato and its implication for breeding. Potato Res 42:283–312

    Google Scholar 

  • Hauck NR, Yamane H, Tao R, Iezzoni A (2002) Self-compatibility and incompatibility in tetraploid sweet cherry (Prunus cerasus L.). Sex Plant Reprod 15:39–46

    Article  Google Scholar 

  • Hayes RJ, Thill CA (2002) Co-current introgression of economically important traits in a potato-breeding program. Am J Potato Res 79:173–181

    Google Scholar 

  • Helgeson JP, Pohlman JD, Austin S, Herbalach GT, Wielgus SM, Ronis D, Zambolim L, Tooley P, McGrath JM, James RV, Stevenson WR (1998) Somatic hydrids between Solanum bullbocastanum and potato: a new source of resistance to late blight. Theor Appl Genet 96:738–742

    Article  Google Scholar 

  • Hermsen JGTh (1978) Genetics of self-incompatibility in dihaploids of Solanum tuberosum L. 2. Detection and identification of all possible incompatibility and compatibility genotypes in six F1s from interdihaploid crosses. Euphytica 27:13–17

    Google Scholar 

  • Hermsen JGTh, Rammana MS (1973) Double-bridge hybrids of Solanum bulbacastanum and cultivars of S. tuberosum. Euphytica 18:27–35

    Google Scholar 

  • Hermsen JGTh, Rammana MS (1976) Barriers to hybridization in of Solanum bulbacastanum Dun. and S. verrucosum Schlechtd. and structural hybridity in their F1 plants. Euphytica 25:1–10

    Google Scholar 

  • Hermsen JGTh, Taylor LM, van Breukelen EWM, Lipski A (1978) Inheritance of genetic markers from two potato dihaploids and their respective parent cultivars. Euphytica 27:681–688

    Google Scholar 

  • Igic B, Kohn J (2001) Evolutionary relationships among self-incompatible RNases. Proc Natl Acad Sci USA 98:1316–13171

    Google Scholar 

  • Janssen GJW, Norel A von, Verkerk-Bakker B, Janssen R, Hoogendoorn J (1997) Introgression of resistance to root-knot nematodes from wild Central American Solanum species into S. tuberosum ssp. Tuberosum. Theor Appl Genet 95:490–496

    Google Scholar 

  • Johnston SA, den Nijs TPM, Peloquin SJ, Hanneman RE Jr (1980) The significance of genic balance to endosperm development in interspecific crosses. Theor Appl Genet 57:5–9

    Google Scholar 

  • Kuhl JC, Havey MJ, Hanneman RE Jr (2002) A genetic study of unilateral incompatibility between diploid (1EBN) Mexican species Solanum pinnatisectum and S. cardiophyllum subsp. cardiophyllum. Sex Plant Reprod 14:305–313

    Article  CAS  Google Scholar 

  • Liedl BE, Anderson NO (1993) Reproductive barriers: identification, uses, and circumvention. Plant Breed Rev 11:11–154

    Google Scholar 

  • Luu DT, Qin X, Laublin G, Yang Q, Morse D, Cappadocia M (2001) Rejection of S-heteroallelic pollen by a dual-specific S-RNase in Solanum chacoense predicts a multimeric SI pollen component. Genetics 159:329–335

    Google Scholar 

  • Matsubayashi M (1991) Phylogenetic relationships in the potato and its related species. In: Tsuchiya T, Gupta PK (eds) Chromosome engineering in plants: genetics, breeding, evolution Part B. Elsevier, Amsterdam, pp 93–118

    Google Scholar 

  • Murfett J, Strabala TJ, Zurek DM, Mou B, Beecher B, McClure BA (1996) S-RNase and interspecific pollen rejection in the genus Nicotiana: multiple rejection pathways pathways contribute to unilateral incompatibility between self incompatible species. Plant Cell 8:943–958

    Article  CAS  PubMed  Google Scholar 

  • Novy RG, Hanneman RE Jr (1991) Hybridization between GP. Tuberosum haploids and 1EBN wild potato species. Am Potato J 68:151–169

    Google Scholar 

  • Novy RG, Nasruddin A, Ragsdale DW, Radcliffe EB (2002) Genetic resistances to potato leafroll virus, potato virus Y, and green peach aphid in progeny of Solanum etuberosum. Am J Potato Res 79:9–18

    Google Scholar 

  • Olsder J, Hermsen JGTh (1976) Genetics of self-compatibility in dihaploids of Solanum tuberosum L. I. Breeding behaviour of two self-compatible dihaploids. Euphytica 25:597–607

    Google Scholar 

  • Ortiz R (1998) Potato breeding via ploidy manipulations. Plant Breed Rev 16:15–86

    Google Scholar 

  • Ramon M, Hanneman RE Jr (2002) Introgression of resistance to late blight (Phytophthora infestans) from Solanum pinnatisectum into S. tuberosum using embryo rescue and double pollination. Euphytica 127:421–435

    Article  CAS  Google Scholar 

  • Spooner DM, Bamberg JB (1994) Potato genetic resources: sources of resistance and systematics. Am Potato J 71:325–337

    Google Scholar 

  • Spooner DM, Hijimans RJ (2001) Potato systematics and germplasm collecting, 1989–2000. Am J Potato Res 78:237–268

    Google Scholar 

  • Zlesak DC, Thill CA (2001) Obtaining sexual hybrids between Solanum pinnatisectum (1EBN) and cultivated potato germplasm. Am J Potato Res 78:489

    Google Scholar 

  • Zlesak DC, Thill CA (2002) Variation for 2n pollen production and male fertility in wild Solanum germplasm resistant to Phytophthora infestans (Mont.) de Bary (US-8). Am J Potato Res 79:219–222

    Google Scholar 

Download references

Acknowledgements

This manuscript is Scientific Journal Series No. 041210136 of the Department of Horticultural Science, University of Minnesota. This research has been supported in part by the University of Minnesota, College of Agriculture, Food, and Environmental Sciences, Minnesota Rapid Agricultural Response Fund, United States Department of Agriculture USDA/ARS grant 59-0500-0-046, Northern Plains Potato Growers Association, and the Minnesota Area II Potato Research and Promotion Council.

Author information

Authors and Affiliations

  1. United States Department of Agriculture, Agricultural Research Service, 1636 E Alisal St, Salinas, CA, 93905, USA

    R.J. Hayes

  2. Department of Horticultural Sciences, University of Minnesota, 305 Alderman Hall, 1970 Folwell Ave, St. Paul, MN, 55108, USA

    I.I. Dinu & C.A. Thill

Authors
  1. R.J. Hayes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I.I. Dinu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C.A. Thill
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C.A. Thill.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hayes, R., Dinu, I. & Thill, C. Unilateral and bilateral hybridization barriers in inter-series crosses of 4x 2EBN Solanum stoloniferum, S. pinnatisectum, S. cardiophyllum, and 2x 2EBN S. tuberosum haploids and haploid-species hybrids. Sex Plant Reprod 17, 303–311 (2005). https://doi.org/10.1007/s00497-005-0244-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00497-005-0244-1

Keywords

Search

Navigation