Abstract
Key message
Minor effect on the chloroplast antioxidant proteins was detected in Eucalyptus urophylla cultivated in high-CO2 atmosphere.
Abstract
Global climate change can significantly alter plant cell metabolism. A higher atmospheric CO2 scenario may be beneficial for C3 plants through the stimulation of photosynthesis. This predicted increase in the rate of carbon assimilation may also increase the expression of enzymes involved in the antioxidant cellular defense. Here, we studied the responses of the chloroplastic antioxidant system of Eucalyptus urophylla plants cultivated in a high-CO2 condition. Plants exposed to a high concentration (980 ppm) of CO2 showed an increase in the H2O2 concentration and MDA content in relation to those cultivated at 410 and 680 ppm. With the discovery proteomics approach used herein, we identified 19 chloroplastic antioxidant proteoforms and pinpointed differentially regulated isoforms of an ascorbate peroxidase and a superoxidase dismutase upon cultivation in a high-CO2 atmosphere. Our data indicate that the CO2 stimulus induces only minor changes in the antioxidant metabolism of E. urophylla chloroplasts.
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References
AbdElgawad H, Zinta G, Beemster GTS, Janssens IA, Asard H (2016) Future climate CO2 levels mitigate stress impact on plants: increased defense or decreased challenge? Front Plant Sci 7:556. https://doi.org/10.3389/fpls.2016.00556
ESRL - Earth System Research Laboratory (2017) national oceanic and atmospheric administration, trends in atmospheric carbon dioxide. https://www.esrl.noaa.gov/gmd/ccgg/trends/full.html. Accessed 16 Oct 2017
Farfan-Vignolo ER, Asard H (2012) Effect of elevated CO2 and temperature on the oxidative stress response to drought in Lolium perenne L. and Medicago sativa L. Plant Physiol Biochem 59:55–62. https://doi.org/10.1016/j.plaphy.2012.06.014
Hu WH, Song XS, Shi K et al (2008) Changes in electron transport, superoxide dismutase and ascorbate peroxidase isoenzymes in chloroplasts and mitochondria of cucumber leaves as influenced by chilling. Photosynthetica 46:581–588. https://doi.org/10.1007/s11099-008-0098-5
IPCC Panel (2014) Climate change: synthesis report. Contribution of working groups I, II and III to the fifth assessment report of the intergovernmental panel on climate change. IPCC, Geneva, pp 73–91
Kumari S, Agrawal M, Tiwari S (2013) Impact of elevated CO2 and elevated O3 on Beta vulgaris L.: Pigments, metabolites, antioxidants, growth and yield. Environ Pollut 174:279–288. https://doi.org/10.1016/j.envpol.2012.11.021
Liu Z, Chen W, He X, Fu S, Lu T (2015) Regulatory effects of elevated carbon dioxide on growth and biochemical responses to ozone stress in Chinese Pine (Pinus tabulaeformis Carr.). Bull Environ Contam Toxicol 97:793–797. https://doi.org/10.1007/s00128-016-1960-y
Michelet L, Zaffagnini M, Morisse A, Sparla F, Pérez-Pérez ME, Francia F, Danon A, Marchand CH, Fermani S, Trost P, Lemaire SD (2013) Redox regulation of the Calvin–Benson cycle: something old, something new. Front Plant Sci 4:270
Mishra P, Bhoomika K, Dubey RS (2013) Differential responses of antioxidative defense system to prolonged salinity stress in salt-tolerant and salt-sensitive Indica rice (Oryza sativa L.) seedlings. Protoplasma 250:3–19. https://doi.org/10.1007/s00709-011-0365-3
Paoletti AC, Parmely TJ, Tomomori-Sato C et al (2006) Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors. Proc Natl Acad Sci USA 103:18928–18933. https://doi.org/10.1073/pnas.0606379103
Singh A, Agrawal M (2015) Effects of ambient and elevated CO2 on growth, chlorophyll fluorescence, photosynthetic pigments, antioxidants, and secondar metabolites of Catharanthus roseus (L.) G Don. grown under three different soil N levels. Environ Sci Pollut Res 22:3936–3946. https://doi.org/10.1007/s11356-014-3661-6
Zlatev ZS, Lidon FC, Ramalho JC, Yordanov IT (2006) Comparison of resistance to drought of three bean cultivars. Biol Plant 50:389–394. https://doi.org/10.1007/s10535-006-0054-9
Acknowledgements
We thank Dra. Priscila Lupino Gratão from the São Paulo State University for providing the laboratory infrastructure for the quantification of H2O2 and MDA content analysis. This work had financial support from São Paulo Research Foundation—FAPESP (Grant number 2015/23354-8) and Coordination for the Improvement of Higher Education Personnel—CAPES.
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Baldassi, A.C., dos Santos, B.M. & Balbuena, T.S. Carbon dioxide induces minor antioxidant responses in Eucalyptus urophylla chloroplasts. Trees 32, 1481–1485 (2018). https://doi.org/10.1007/s00468-018-1750-8
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DOI: https://doi.org/10.1007/s00468-018-1750-8