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Characteristics of Acacia mangium shoot apical meristems in natural and in vitro conditions in relation to heteroblasty

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Abstract

Morphological and histocytological characteristics of Acacia mangium shoot apical meristems (SAMs) were assessed in natural and in vitro conditions in relation to heteroblasty. In the natural environment, SAMs with a mature-phyllode morphology were much bigger, contained more cells with larger vacuolated area, or vacuome, and lower nucleoplasmic ratios than those from the juvenile type (Juv). In these latter, nuclei appeared more voluminous, evenly and lightly stained, with clearly distinguishable nucleolei and less abundant chromocenters. In vitro, where reversions from mature to juvenile morphological traits do occur unpredictably, heteroblasty was less obvious in the SAM characteristics examined. In vitro SAMs corresponding to the juvenile and mature types showed similarities with outdoor Juv SAMs, but could be distinguished from these latter by a much larger vacuome that might be induced by the culture conditions. These findings encourage pursuing the investigations at the chromatin and nucleolus level in SAM zones where heteroblasty-related differences have been detected.

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Acknowledgments

The authors are indebted to ATP CIRAD “Méristèmes” for financial contribution to this study.

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Authors and Affiliations

  1. CIRAD-BIOS, UMR AGAP PHIV-MRI, TA 40/02 Avenue Agropolis, 34398, Montpellier Cedex 5, France

    Clemence Hatt, Fabienne Montes, Marc Lartaud & Jean-Luc Verdeil

  2. CRDPI, B.P 1291, Pointe-Noire, Congo

    François Mankessi

  3. CIRAD-BIOS, UMR AGAP and INRIA, Virtual Plants, TA A-108/02, Avenue Agropolis, 34398, Montpellier Cedex 5, France

    Jean-Baptiste Durand & Frédéric Boudon

  4. CIRAD-BIOS, UMR AGAP, TA A-108/03-Avenue Agropolis, 34398, Montpellier Cedex 5, France

    Clemence Hatt & Olivier Monteuuis

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  1. Clemence Hatt
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  2. François Mankessi
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Correspondence to Olivier Monteuuis.

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Communicated by K. Klimaszewska.

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Hatt, C., Mankessi, F., Durand, JB. et al. Characteristics of Acacia mangium shoot apical meristems in natural and in vitro conditions in relation to heteroblasty. Trees 26, 1031–1044 (2012). https://doi.org/10.1007/s00468-012-0680-0

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