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Effect of chilling on photosynthesis and antioxidant enzymes in Hevea brasiliensis Muell. Arg.

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Abstract

The aim of the present study was to assess the tolerance of Hevea brasiliensis to chilling temperatures since rubber production has been extended to sub-optimal environments. PB260 clone was used to analyze the responses of leaves chilled at 10°C during 96 h, as well as their recovery at 28°C. Some key parameters were used to evaluate photosynthetic apparatus functioning, membrane damage (electrolyte leakage) and oxidative stress. A short-term response versus a long-term one have been recorded, the time point of 24 h, when stomata closure was effective, being the border between the two responses. P n decreased dramatically at 1 h, and Fv/Fm was slightly affected. NPQ reached its maximal level between 4 and 7 h. Lipid peroxidation and membrane lysis were observed between 48 and 96 h. Activities of antioxidant enzymes increased, along with the induction of antioxidant gene expression. Finally, the plants were capable to recover (net photosynthetic rate, photochemical efficiency, antioxidant enzymes activities) when placed back to 28°C showing that PB260 can withstand long-term chilling.

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Acknowledgments

We are indebted to Dr. David Biron (Canadian scientist) for English grammar and syntax corrections. We are very grateful to the two anonymous referees for their insightful comments which substantially improved this paper. This work was supported in part by MICHELIN (Clermont-Ferrand, France).

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Authors and Affiliations

  1. UMR547 PIAF, Université Blaise Pascal, 24 av des Landais, 63177, Aubière, France

    Jing Mai, Stéphane Herbette, Jean-Louis Julien & Patricia Roeckel-Drevet

  2. UMR547 PIAF, INRA, 134 av du Brézet, 63100, Clermont-Ferrand, France

    Marc Vandame & Thierry Améglio

  3. CIRAD, UPR Ecosystèmes de plantations, Research and Development Building, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand

    Boonthida Kositsup

  4. Department of Horticulture, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand

    Boonthida Kositsup & Poonpipope Kasemsap

  5. Michelin, CPN/A, Ladoux, 63040, Clermont-Ferrand Cedex 9, France

    Eric Cavaloc

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  1. Jing Mai
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Correspondence to Patricia Roeckel-Drevet.

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Mai, J., Herbette, S., Vandame, M. et al. Effect of chilling on photosynthesis and antioxidant enzymes in Hevea brasiliensis Muell. Arg.. Trees 23, 863–874 (2009). https://doi.org/10.1007/s00468-009-0328-x

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