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Effect of Mn toxicity on morphological and physiological changes in two Populus cathayana populations originating from different habitats

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Abstract

The cuttings of Populus cathayana were exposed to four different manganese (Mn) concentrations (0, 0.1, 0.5 and 1 mM) in a greenhouse to investigate the toxicity of Mn and the detoxifying responses of woody plants. Two contrasting populations of P. cathayana, which were from wet and dry climate regions in western China, respectively, were examined in our study. The results showed that high concentration of Mn caused significant decrease in shoot height, biomass accumulation, and leaf number and leaf areas. Injuries to the anatomical features of leaves were also found as the reduced thickness of palisade and spongy parenchyma, the decreased density in the conducting tissue and the collapse and split in the meristematic tissue in the central vein. Moreover, Mn treatments caused the accumulation of hydrogen peroxide (H2O2), and then resulted in oxidative stress indicated by the oxidation of proteins and DNA. Many physiological responses were employed to cope with the toxicity of Mn, including the increase in the contents of non-protein thiol (NP-SH), phytochelatins (PCs) and phenolics compounds and the stimulated activities of guaiacol peroxidase (GPX) and polyphenol oxidase (PPO) for the chelation of Mn and for the antioxidation of reactive oxygen species. The population from dry climate habitat showed a lower leaf concentration of Mn, higher contents of the chelators, and higher activities of GPX and PPO than did the wet climate population at the same Mn treatment, thereby possessing a superior Mn tolerance. In both populations, most of the Mn was accumulated in the shoot, which is favorable regarding phytoremediation.

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Acknowledgments

The research was supported by the Outstanding Young Scientist Program of the National Natural Science Foundation of China (No. 30525036) and the China National Key Program of the International Cooperation for Science and Technology (No. 2005DFA30620).

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Authors and Affiliations

  1. Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Yunnan, 666303, China

    Yanbao Lei

  2. Chengdu Institute of Biology, Chinese Academy of Sciences, P.O.Box 416, Chengdu, 610041, China

    Yanbao Lei, Ke Chen & Chunyang Li

  3. School of Biological Resources and Environmental Sciences, Jishou University, Hunan, 416000, China

    Xiangrong Tian

  4. Department of Applied Biology, University of Helsinki, P.O. Box 27, 00014, Helsinki, Finland

    Helena Korpelainen

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  1. Yanbao Lei
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  2. Ke Chen
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  5. Chunyang Li
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Correspondence to Chunyang Li.

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Communicated by T. Hogetsu.

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Lei, Y., Chen, K., Tian, X. et al. Effect of Mn toxicity on morphological and physiological changes in two Populus cathayana populations originating from different habitats. Trees 21, 569–580 (2007). https://doi.org/10.1007/s00468-007-0152-0

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