Abstract
RAPD (Random Amplified Polymorphic DNA) fingerprints have recently been used to estimate genetic and taxonomic relationships in plants. In this study RAPD analysis was performed on 32 clones belonging to different species of the genus Populus. Of these, 25 clones are registered in several countries for commercial use and, altogether, cover almost 50% of the worlds cultivated poplars. DNA was prepared from leaves and amplified by PCR using random oligonucleotide primers. Amplification products were separated by agarose-gel electrophoresis to reveal band polymorphisms. Four primers out of the 18 tested, were selected on the basis of the number and frequency of the polymorphisms produced. With these a total of 120 different DNA bands were reproducibly obtained, 92% of which were polymorphic. The polymorphisms were scored and used in band-sharing analyses to identify genetic relationships. With a few but interesting exceptions, these are consistent with the present taxonomy of the genus Populus and with the known predigrees of cultivated poplars. Moreover, the results show that RAPD analysis allows one to discriminate among all tested clones and can, therefore, be recommended as a convenient tool to defend plant breeders rights.
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References
Bailey DC (1983) Isozymic variation and plant breeders' rights. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part A. Elsevier, Amsterdam, pp 425–441
Boccone A (1975) Differenze chemiotassonomiche tra specie e cloni di pioppo a livello del contenuto in flavoni. Cellulosa e Carta 27:89–46
Bortitz S (1962) Papierchromatographische Differenzierung einiger Arten und Sorten der Gattung Populus. Züchter 32:24–33
Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331
Caetano-Anollés G, Bassam BJ, Gresshoff PM (1991) DNA amplification fingerprint using very short arbitrary oligonucleotide primers. Biotechnology 9:553–557
Devos KM, Gale MD (1992) The use of random amplified polymorhic DNA markers in wheat. Theor Appl Res 84:567–572
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Draper SR, Keefe PD (1989) Machine vision for the characterization and identification of cultivars. Plant Var Seeds 2:53–62
Erlich HA, Gelfand D, Sninsky JJ (1991) Recent advances in the polymerase chain reaction. Science 252:1643–1650
FAO (1971) Rapport du Sous-Comitè d'enregistrement des noms du peuplier. FAO/CIP/123, Rome
FAO (1980) Poplars and willows. FAO, Rome
Greenway W, Jobling J, Scaysbrook T (1989) Composition of bud exudates of Populus x interamericana clones as a guide to clonal identification. Silvae Genet 38:29–32
Halward T, Stalker T, LaRue E, Kochert G (1992). Use of single-primer DNA amplifications in genetic studies of peanut (Arachis hypogaea L.). Plant Mol Biol 18:315–325
Hamill JD, Rounsley S, Spencer A, Todd G, Rhodes MJC (1991) The use of the polymerase chain reaction in plant transformation studies. Plant Cell Rep 10:221–224
Helentjaris T, King G, Slocum M, Siedenstrang C, Wegman S (1985) Restriction fragment polymorphisms as probes for plant diversity and their development as tools for applied plant breeding. Plant Mol Biol 5:109–118
Houtzagers G (1950) II genere Populus e la sua importanza nella selvicolutra. Biblio Sci Cartiere Burgo Casale Monferrato, Italia
Hu CC, Crovello TJ, Sokall RR (1985) The numerical taxonomy of some species of Populus based only on vegetative characters. Taxon 34:197–206
Hu J, Quiros CF (1991) Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep 10:505–511
Jayarao BM, Bassam BJ, Caetano-Anollés, Grésshoff PM, Oliver SP (1992) Subtyping of Streptococcus uberis by DNA amplification fingerprinting. J Clin Microbiol 30:1347–1350
Landry BS, Kesseli R, Hei Leung, Michelmore RW (1987) Comparison of restriction endonucleases and sources of probes for their efficiency in detecting restriction fragment length polymorphisms in lettuce (Lactuca sativa L.). Theor Appl Genet 74:646–653
Lapietra G (1992) Principali caratteristiche dei phi noti cloni di pioppo selezionati in Italia. L'Informatore Agrario 18:83–86
Martin GB, Williams JG, Tanksley SD (1991) Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines. Proc Natl Acad Sci USA 88:2336–2340
Rajora OP (1988) Allozymes as aids for identification and differentiation of some Populus maximowiczii Henry clonal varieties. Biochem Syst Ecol 16:635–640
Rajora OP (1989) Characterization of 43 Populus nigra L. clones representing selections, cultivars and botanical varieties based on their allozyme genotypes. Euphytica 43:197–206
Rajora OP, Zsuffa L (1989) Multilocus genetic structure, characterization and relationships of Populus x canadensis cultivars. Genome 32:99–108
Riedy MF, Hamilton WJ, Aquadro CF (1992) Excess of nonparental bands on offspring from known primate pedigrees assayed using RAPD-PCR. Nucleic Acids Res 20:918
Sneath PHA, Sokal RR (1973) Numerical taxonomy. The principles and practice of numerical classification. W. H. Freeman, San Francisco
Tanksley SD, Young DN, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Biotechnology 7:257–264
UPOV (1981) Guidelines for the conduct of tests for distinctness, homogeneity and stability: poplar (Populus L.). UPOV, TG/21/7
Vivani W, Sekawin M (1953) Esperienze di poliploidia indotta nel genere Populus L. FAO/IPC, 2nd Session, Roma, Italy
Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218
Welsh J, Honeycutt RJ, McClelland M, Sobral BWS (1991) Parentage determination in maize hybrids using the arbitrarily primed polymerase chain reaction (AP-PCR). Theor Appl Genet 82:473–476
Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
Wyman AR and White R (1980) A highly polymorphic locus in human DNA. Proc Natl Acad Sci USA 77:6754–6758
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Communicated by F. Salamini
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Castiglione, S., Wang, G., Damiani, G. et al. RAPD fingerprints for identification and for taxonomic studies of elite poplar (Populus spp.) clones. Theoret. Appl. Genetics 87, 54–59 (1993). https://doi.org/10.1007/BF00223744
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DOI: https://doi.org/10.1007/BF00223744