Introduction

Autosomal recessive polycystic kidney disease (ARPKD) is a rare but severe disease with a high mortality rate and increased morbidity leading to childhood chronic kidney failure in approximately 50% of children [1, 2]. It is primarily a tubular disorder, and it affects mainly children, but late manifestations in adulthood also exist [3, 4]. Kidney concentrating ability test is a simple measure of the function of the collecting duct and distal tubule, which has been demonstrated to be impaired in about half of adults, as well as pediatric patients with autosomal dominant polycystic kidney disease (ADPKD) [5,6,7,8]. However, there has been only one small study on the concentrating capacity in patients with ARPKD from 1975, which was performed on five children [9]. There is also a finding from an experimental study on a rat model of ARPKD that showed impaired urine concentrating capacity in these animals [10].

We aimed to study kidney concentrating capacity in a larger cohort of children with ARPKD and to elucidate the possible association between concentrating capacity, glomerular filtration rate, blood pressure (BP), hypertension, and kidney size.

Patients and methods

Forty-nine patients with ARPKD were managed from 1996 to 2020 within our three tertiary pediatric kidney clinics in Prague, Bratislava, and Munich. We have retrospectively identified from medical records 18 patients with ARPKD who had urine concentrating capacity performed (n = 14/3/1 from Prague/Bratislava/Munich). The demographic and clinical data, antihypertensive medication, symptoms, and complications of children with ARPKD are shown in Table 1. Thirteen patients were described in our earlier paper on ambulatory blood pressure in ARPKD [11].

Table 1 Characteristics of the patients with ARPKD. Data are medians and interquartile range (IQR)

The diagnosis of ARPKD was established using phenotype characteristics (typical kidney ultrasound with enlarged hyperechogenic kidneys with poor corticomedullary differentiation with/without small cysts) and characteristic hepatic involvement with liver fibrosis). These criteria were completed together with normal kidney ultrasounds from both parents, consistent with autosomal-recessive inheritance of kidney disease [2]. Moreover, in eleven of these eighteen patients, pathogenic homozygous or compound heterozygous mutations of the PKHD1 gene were detected. In the remaining seven patients, genetic analysis was not done.

Kidney concentrating capacity

We performed a morning standardized kidney concentrating ability test according to Janda et al.; urine osmolality was measured four hours after intranasal application of 5 μg/5 kg body weight 1-deamino-8-D-arginine-vasopressin – desmopressin (DDAVP, Ferring, CZE) [12]. Any amount of fluid intake was disallowed for 4 h and urine osmolality was measured after the application of desmopressin in the urine collected during this 4-h period (Roeblig osmometer was used and urine osmolality expressed in mOsmol/kg). There were no children who suffered from rhinitis or acute intercurrent illness at the time we performed the kidney concentrating test and no complications were observed while performing the investigation. Impaired kidney concentrating ability was defined as urine osmolality < 900 mOsmol/kg [12].

Glomerular filtration

Serum creatinine was measured on the same day as the administration of desmopressin (Jaffé or enzymatic). The glomerular filtration rate (GFR) was estimated by Schwartz formula [13, 14]. Chronic kidney disease (CKD) stages were classified according to Kidney Disease Improving Global Outcomes recommendations [15].

Blood  pressure

Office blood pressure (BP) was measured on the same day as the administration of desmopressin using a conventional mercury manometer, while hypertension on the other hand was diagnosed as systolic and/or diastolic BP ≥ 95th percentile, according to appropriate recent European recommendations [16],or taking antihypertensives. Sixteen children received antihypertensives: 14 angiotensin−converting enzyme inhibitors, 1 angiotensin receptor blocker, 9 beta−blockers, 9 calcium channel blockers, and 1 diuretic. The office blood pressure index (BPI) was calculated as BP divided by the 95th percentile. The heaviness of hypertension (HT) was staged by a modified hypertension score according to Guidi et al. [17]. The hypertension scores were calculated for every child; each antihypertensive = one point and hypertension discovered through office BP measurements, regardless of drugs administered = 1 point.

Kidney  ultrasound

A kidney ultrasound was performed at the same time, or up to 6 months before or after the desmopressin test (5−MHz transducers: either an Acuson 128 PX−10 Siemens Medical Solutions, Erlangen, Germany or a 270 SSA: Toshiba Medical Systems, Tokyo, Japan). The size of the kidney (mm) was expressed as standard deviation score (SDS) [18].

Ethical considerations

The present trial was conducted in accordance with the principles outlined in the Declaration of Helsinki. In view of the retrospective nature of the study, ethical approval was waived by the local ethics committee at the University Hospital Motol in Prague and Bratislava and all procedures performed were part of the routine care of patients with ARPKD.

Statistical analysis

As the variables were irregularly distributed, all continuous variables were expressed using medians and interquartile ranges; Wilcoxon test was used for univariate comparison. The Spearman rank correlation test was used to assess correlations between continuous variables. Multivariate regression analysis was performed using urine osmolality as an outcome variable along with antihypertensive scores, with blood pressure and eGFR as predictor variables. All statistical tests were done using R statistical software; p < 0.05 was regarded to be statistically significant.

Results

Kidney concentrating ability was shown to be impaired in all 18 children (100%). Median urine osmolality was 360 mOsmol/kg (IQR 302–459). Data of urine osmolality of individual patients are given in Fig. 1.

Fig. 1
figure 1

Kidney concentrating capacity in children with ARPKD (dotted line represents the lower limit of normal concentrating capacity)

Twelve children (67%) were defined as having CKD stages 2–4 with decreased eGFR. The median concentrating capacity was significantly higher in children with normal GFR (CKD 1) when compared to children with CKD stages 2–4 (544 mOsmol/kg, IQR 484–602 vs. 327 mOsmol/kg, IQR 281–396, p = 0.002, Fig. 2).

Fig. 2
figure 2

Kidney concentrating capacity in children with CKD1 and CDK stages 2–4

The median concentrating capacity was not significantly different between children with early (< 1 year, n = 8) or late (> 1 year, n = 10) initial clinical presentation (397 mOsmol/kg, IQR 325–465 vs. 401 mOsmol/kg, IQR 384–564, p < 0.05).

Thirteen children (72%) were on antihypertensive drugs at the time of the study. Sixteen children (89%) were defined as hypertensive based on actual office BP levels or the use of antihypertensive drugs. The median systolic BPI was 0.99 (IQR 0.96–1.16) and diastolic BPI 0.96 (IQR 0.85–1.15); the median hypertension score was 2 (IQR 1–3). Eleven children (61%) were defined as hypertensive based on actual office BP levels, regardless of the use of antihypertensive drugs. The median concentrating capacity was significantly lower in children with hypertensive actual BP (BP index ≥ 95th percentile regardless of drugs) when compared to children with normotensive actual office BP (353 mOsmol/l, IQR 329–402 vs. 472 mOsmol/kg, IQR 282–546, p < 0.05).

Correlations between kidney concentrating capacity and different parameters are shown in Table 2. The correlation between concentrating capacity was significant for eGFR (Fig. 3) and hypertension score (Fig. 4), but not for kidney length, age, or proteinuria. The multivariate model showed eGFR as the independent predictor of kidney concentrating capacity (estimate standard error − 188.75, p = 0.01).

Table 2 Correlations between kidney concentrating capacity and clinical and laboratory parameters in children with ARPKD
Fig. 3
figure 3

Correlation between kidney concentrating capacity and eGFR

Fig. 4
figure 4

Kidney concentrating capacity in patients with different hypertension scores (hypertension score: 1 antihypertensive drug = 1 point, 2 drugs = 2 points etc.; hypertension by BP measurement regardless of drugs = 1 point)

Discussion

Autosomal recessive polycystic kidney disease is a very rare (1:20,000 live births) but severe multisystemic, typically early-onset disease. It can lead to perinatal mortality in 20–30% of newborns and neonatal survivors throughout childhood, with chronic kidney failure prevalence in approximately 50% of them [1, 2]. The most common clinical complication besides chronic kidney disease and congenital hepatic fibrosis is arterial hypertension; this affects about 90% of patients with ARPKD [10]. As it is primarily a tubular disorder (mainly collecting duct), different signs of tubulopathy such as impaired urinary dilution capacity associated with hyponatremia are present in 6–26% of children with ARPKD [2, 19].

In our previous study [6] along with further pediatric and adult studies [5, 7, 8, 20, 21], it has been shown that approximately 50% of patients with ADPKD have impaired kidney concentrating ability and reduced concentration capacity. These impairments were linked to ambulatory BP and the number of kidney cysts, but not to GFR. These findings have shown an association between kidney structural anomalies such as count of kidney cysts and functional anomalies of the tubuli such as impaired kidney concentrating ability.

Contrary to several studies regarding concentrating capacity in patients with ADPKD, to the best of our knowledge, there is only one small study on kidney concentrating capacity in patients with ARPKD. In this 1975 study, Anand et al. investigated urinary concentrating ability following vasopressin administration in five children with infantile polycystic kidney disease, an old term for ARPKD [9]. They demonstrated that the urinary concentrating ability was impaired in all five children (100%). They hypothesized that the inability to excrete maximally concentrated urine could be caused by anomalies in the tubular cells or changes in medulla due to increasing cysts.

In another clinical study on ARPKD, Kaplan et al. showed spontaneous urine osmolality in eight hyponatremic children and reported that it was inappropriately high at 119–220 mOsmol/kg [19].

In an animal model of a rat that phenotypically resembles ARPKD, Guay-Woodford et al. showed impaired urine-concentrating capacity [10]. At 3 weeks of age, the rats exhibited low urine osmolality after 2 h of fasting, along with elevated plasma creatinine and proteinuria, while showing parallel decreased glomerular filtration. Interestingly, urine osmolality was very similar (394 mOsmol/kg) to the urine osmolality in the children of our current study (389 mOsmol/kg) and three times lower than in unaffected animals (961 mOsmol/kg).

Our study is the second and largest study on kidney concentrating capacity of patients with ARPKD. It showed that 100% of ARPKD children have a decreased concentrating capacity; this impairment is thus more severe than in children with ADPKD (mean urine osmolality 389 mOsmol/kg in ARPKD vs. 882 mOsmol/kg in ADPKD) [6]. The significant correlation between concentrating capacity with eGFR is suggestive of an association between tubular and glomerular function in children with ARPKD, and eGFR was an independent predictor of kidney concentrating capacity. We could not find any similar correlation in patients with ADPKD in our previous study [6]. The reason for this difference in both polycystic kidney diseases could be the fact that all children with ADPKD had normal GFR and this thereby precludes any association due to the lack of children with decreased GFR. The difference can also be explained by the different affected nephron segments in ADPKD (all nephron segments) and ARPKD (predominant collecting duct where the final stage of urine concentration takes place). A correlation between concentrating capacity with GFR, similar to ARPKD, has also been demonstrated in non-genetic CKD, such as in patients with posterior urethral valves or reflux nephropathy [22, 23].

The children with ARPKD have a defect in both urine-diluting and urine-concentrating capacities [2, 9]. With disease progression, the microcystic lesions predominantly involve the cortical and medullary collecting ducts, causing further impairment of urine concentrating capacity.

The kidney concentrating capacity test has been performed in many other genetic and non-genetic CKD conditions, such as vesicoureteral reflux, posterior urethral valves, post-acute pyelonephritis, hypercalciuria, Bartter syndrome, Fanconi syndrome, tubulointerstitial nephritis, and post-hemolytic-uremic syndrome [22,23,24,25,26,27,28,29]. The tests showed impaired concentrating ability in a substantial proportion of children; therefore, it can be stated that a described concentration defect is not specific to ARPKD but is a nonspecific marker of distal tubular defect.

In our previous study on children with ADPKD, we found an association between ambulatory BP and kidney concentrating capacity [6]. In our current study, however, we did not find any similar association with office BP. As a possible explanation of the lack of correlation between BP and concentrating capacity in ARPKD, in comparison to children with ADPKD, we could ascertain that it might be the superior accuracy of 24 h ABPM regarding the reflection in the real BP of the patients. This may have been improved by including repeated office BP measures or home BP measurements rather than single measurements. Nevertheless, we have found a weak but significant correlation between concentrating capacity and hypertension score. This further exemplifies the heaviness of hypertension using the hypertensive score that also includes antihypertensive therapy rather than using only actual office BP, which can be decreased by the antihypertensives. Therefore, despite the lack of association between actual BP and kidney concentrating capacity, the association with hypertension continues to score further points. This might be explained by the fact that children with an elevated BP and hypertension have a more progressed state of ARPKD. In adults as well as pediatric patients with ADPKD, the concentrating capacity is linked to BP and hypertension [30, 31]. Contrary to the children with ARPKD, no correlation was found with actual BP. This could be related to the fact that the majority of children were treated with antihypertensive drugs, which preclude a correlation between tubular function and BP as in ADPKD children. Therefore, we used a hypertension score by Guidi et al. [17] to quantify the severity of hypertension more accurately, rather than solely considering the actual BP level.

Contrary to the children with ADPKD, we could not demonstrate any correlation between concentrating capacity and kidney length in ARPKD children. This could be due to the low accuracy and sensitivity of ultrasound derived kidney length or the fact that the relative kidney size (given as SDS) decreases relatively as the children are growing and the disease is progressing. Therefore, unlike in ADPKD, kidney size does not correlate with glomerular rates as well as tubular kidney functions in ARPKD [32].

It is very unlikely that the impaired concentrating capacity would be caused by antihypertensive therapy with diuretics, as only one patient received this drug that can influence the urinary concentrating capacity.

There are several possible pathophysiological mechanisms of impaired kidney concentrating capacity in ARPKD, such as structural alteration of the distal tubules and collecting ducts due to cyst formation, alterations in vasopressin receptor (V2R), ion channels such as epithelial sodium channels (ENaC) or aquaporins (AQPR) [33].

Impaired concentrating capacity that is present in children with ARPKD could have direct clinical relevance for patients, such as the increased risk of dehydration and/or a need for higher water intake or risk for enuresis.

There are several limitations to our study. Firstly, its retrospective design can produce a possible selection bias in the results. However, the kidney concentrating capacity test was performed in the majority of the children as part of the routine investigation of tubular function in children with tubular disorders such as ARPKD. Secondly, the small number of patients can hamper the statistical power. Therefore, our small retrospective study could not answer the question whether impaired concentrating capacity is truly an independent risk factor for progression of the disease. Future larger prospective studies would be beneficial and need to be performed in order to answer this question.

In conclusion, this second and largest study on kidney concentrating capacity in humans with ARPKD showed that it is decreased in most children with this disorder and is linked to glomerular filtration and hypertension but not to kidney size or proteinuria.