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Effect of laparoscopic liver resection versus the open technique on hepatocyte regenerating activity in the rat

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Abstract

Background

Laparoscopic liver resection offers a safe and feasible option primarily for the excision of hepatic neoplasms. Timely recovery of liver volume is a key factor for improving prognosis and post-operative mortality of patients undergone liver resection. The aim of the present study was to compare liver regeneration after laparoscopic over open partial hepatectomy.

Methods

Wistar rats were subjected to laparoscopic 70% hepatectomy (group LAP-HEP), open 70% hepatectomy (group HEP), sham operation (group Sham) or no intervention (group Control). At various timepoints following operation (1 h–2 weeks), the liver was excised to assess relative liver weight, thiobarbituric acid reactive substances (TBARS) levels, mitotic activity, tissue expression of Nuclear Factor-κB (NFκB), Intercellular Adhesion Molecule-1 (ICAM-1) and Vascular Cell Adhesion Molecule-1 (VCAM-1) and the histopathologic profile.

Results

No differences were seen in relative liver weight between hepatectomy groups. Mitotic index was increased in all operative study groups, being higher in group LAP-HEP than in group HEP. TBARS levels were higher in group LAP-HEP compared to group HEP. NFκB and VCAM-1 tissue expression scores were increased in all operative study groups with VCAM-1 being higher in group HEP, while ICAM-1 was overexpressed only in hepatectomy groups. Mild histopathologic lesions were noted in hepatectomy groups with the histopathologic score being higher in group HEP (24 h).

Conclusions

Laparoscopic liver resection enhanced hepatocyte mitotic activity which was accompanied by mild oxidative stress and a less pronounced local inflammatory response and tissue injury to that of the open technique.

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Funding

This work was supported by a Grant of the Special Account for Research Funds of the Democritus University of Thrace (Code No 80216).

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Authors and Affiliations

  1. Laboratory of Experimental Research and Surgical Research, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece

    Petros Ypsilantis, Eleni Panidou-Tsoulou, Orestis Ioannidis, Albion Totsi, Michael Pitiakoudis & Constantinos Simopoulos

  2. Laboratory of Histology and Embryology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece

    Maria Lambropoulou

  3. Laboratory of Biochemistry, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece

    Konstantinos Anagnostopoulos

Authors
  1. Petros Ypsilantis
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  2. Maria Lambropoulou
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  3. Konstantinos Anagnostopoulos
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  4. Eleni Panidou-Tsoulou
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  5. Orestis Ioannidis
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  6. Albion Totsi
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  7. Michael Pitiakoudis
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  8. Constantinos Simopoulos
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Correspondence to Petros Ypsilantis.

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Disclosure

Petros Ypsilantis, Maria Lambropoulou, Konstantinos Anagnostopoulos, Eleni Panidou-Tsoulou, Orestis Ioannidis, Albion Totsi, Michael Pitiakoudis and Constantinos Simopoulos declare that they have no conflict of interest or financial ties to disclose.

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Ypsilantis, P., Lambropoulou, M., Anagnostopoulos, K. et al. Effect of laparoscopic liver resection versus the open technique on hepatocyte regenerating activity in the rat. Surg Endosc 34, 4812–4817 (2020). https://doi.org/10.1007/s00464-019-07257-0

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  • DOI: https://doi.org/10.1007/s00464-019-07257-0

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