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Liver metastasis and ICAM-1 mRNA expression in the liver after carbon dioxide pneumoperitoneum in a murine model

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Abstract

Background

Liver metastasis of colorectal malignancies is an important prognostic factor. Several studies have demonstrated that carbon dioxide (CO2) pneumoperitoneum enhances liver metastasis in animal models. Little is known about intercellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor-alpha (TNF-(α) mRNA expression in the liver after CO2 pneumoperitoneum.

Methods

Forty-five male BALB/c mice were randomly divided into three groups after intra-splenic tumor cell (colon 26) inoculation and the following procedures were performed: CO2 pneumoperitoneum (n = 15), open laparotomy (n = 15), and anesthesia alone (n = 15). On day 7 after each procedure, the livers were excised and the number and diameter of the tumor nodules and the cancer index score were determined. Another 90 male BALB/c mice were randomly divided into three groups as described above, and they underwent each procedure (n = 30 each). After each procedure, the livers were excised on days 0, 1, 3, and ICAM-1 and TNF-α mRNA expression were examined by real-time RT-PCR using SYBR Green I.

Results

The number of tumor nodules and the cancer index score were larger in the CO2 pneumoperitoneum group than in the control group (p < 0.05). The mean diameter of the tumor nodules was not different among the three groups. The expression of ICAM-1 in the CO2 pneumoperitoneum group was higher than that in the other groups on day 1 (p < 0.05), and the TNF-α mRNA was higher than that in the control group on day 1 (p < 0.05).

Conclusions

CO2 pneumoperitoneum enhances liver metastasis compared with anesthesia alone, and ICAM-1 expression in the liver after the pneumoperitoneum plays an important role in establishing liver metastasis in a murine model.

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Acknowledgement

This study was supported in part by a Grant-in-Aid for Scientific Research (no. 15390401) from the Japanese Ministry of Education, Science, and Culture.

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Authors and Affiliations

  1. Department of Surgery I, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama-machi, Oita, 879-5593, Japan

    K. Izumi, K. Ishikawa, M. Tojigamori, Y. Matsui, N. Shiraishi & S. Kitano

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  1. K. Izumi
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  2. K. Ishikawa
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  3. M. Tojigamori
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  4. Y. Matsui
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Correspondence to K. Izumi.

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Izumi, K., Ishikawa, K., Tojigamori, M. et al. Liver metastasis and ICAM-1 mRNA expression in the liver after carbon dioxide pneumoperitoneum in a murine model. Surg Endosc 19, 1049–1054 (2005). https://doi.org/10.1007/s00464-004-2177-4

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  • DOI: https://doi.org/10.1007/s00464-004-2177-4

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