Non conventional protease purification methods for large scale application

Abstract

Studies on the cost effective purification method is very much essential for the industrially important enzyme like protease. The effects of different parameters studied on purification efficiency by non-conventional methods. The first process based on the adsorption property of activated charcoal and the next process was treatment with H₂O₂ followed by acetone precipitation. In this way about 65.5% of protease recovery with 4.5 fold purification was achieved. Based on laboratory data scaled up studies were made.