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Kinetic and stoichiometric parameters in the fed-batch bioreactor production of poly(3-hydroxybutyrate) by Bacillus megaterium using different carbon sources

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Abstract

This study investigates the effects of different strategies on poly(3-hydroxybutyrate)-P(3HB) production in a fed-batch bioreactor by Bacillus megaterium using candy industry effluent (CIE), sucrose, and rice parboiled water (RPW) as carbon sources. In biosynthesis, kinetic and stoichiometric parameters of substrate conversion into products and/or cells, productivity, instantaneous, and specific conversion rates were evaluated. The maximum concentration of P(3HB) was 4.00 g.L−1 (77% of the total dry mass) in 42 h of cultivation in minimal medium/RPW added with a carbon source based on CIE, demonstrating that the fed-batch provided an increase of approximately 22% in the polymer concentration and 32% in the overall productivity in relation to medium based on commercial sucrose. Fed-batch cultivation also had the advantage of avoiding the extra time required for inoculum preparation and sterilization of the bioreactor during the batch, which thereby increased the overall industrial importance of the process. Effluents from the candy, confectionery, and/or rice parboiling industries can be used as alternative substrates for P(3HB) production at a low cost.

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The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

This study was financed in part by the National Council for Scientific and Technological Development—Brazil (CNPq), Coordination for the Improvement of Higher Education Personnel—Brazil (CAPES)—Finance Code 001 and Research Support Foundation of the State of Rio Grande of Sul—Brazil (FAPERGS).

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Authors and Affiliations

  1. Department of Food Engineering, URI - Erechim, Av. Sete de Setembro, 1621, Erechim, RS, 99709-910, Brazil

    Rafaela Nery De Melo, Guilherme de Souza Hassemer do, Lucas Henrique Nascimento, Rosicler Colet, Clarice Steffens, Alexander Junges & Eunice Valduga

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  1. Rafaela Nery De Melo
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  2. Guilherme de Souza Hassemer do
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Correspondence to Clarice Steffens.

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De Melo, R.N., de Souza Hassemer do, G., Nascimento, L.H. et al. Kinetic and stoichiometric parameters in the fed-batch bioreactor production of poly(3-hydroxybutyrate) by Bacillus megaterium using different carbon sources. Bioprocess Biosyst Eng 46, 1791–1799 (2023). https://doi.org/10.1007/s00449-023-02935-9

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