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Green synthesis of zinc and nickel dual-doped cerium oxide nanoparticles: antioxidant activity and cytotoxicity effects

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Abstract

Cerium oxide nanoparticles (CeO2-NPs) and Zn–Ni dual-doped CeO2-NPs were synthesized through a green approach by the implication of zucchini peel (Cucurbita pepo) extract as a capping and reduction agent. All the synthesized samples were studied by the results of FTIR, UV–Vis, XRD, and FESEM/EDAX/PSA analyses. The Zn–Ni dual-doped CeO2-NPs contained a spherical morphology and their size was observed to increase at higher temperatures. The conducted MTT assay on the Huh-7 cell line displayed 50% of cells annihilation as a result of using undoped CeO2-NPs and Zn–Ni dual-doped CeO2-NPs at the inhibitory concentrations (IC50) of 700 and 185.4 μg/mL, respectively. We also evaluated the enzymatic functionality of SOD and CAT of undoped CeO2-NPs and dual-doped NPs and found it to be dose dependent. Moreover, Zn–Ni dual-doped CeO2-NPs intensified the CAT activity without causing any changes in SOD activity in similar concentrations.

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Abbreviations

DMEM:

Dulbecco’s modified Eagle’s medium

DMSO:

Dimethyl sulfoxide

PBS:

Phosphate-buffered saline

FBS:

Fetal bovine serum

MB:

Methylene blue

IC50 :

Half-maximal inhibitory concentration

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide

DTA:

Thermogravimetric analysis (TGA) and differential thermal analysis

FT-IR:

Fourier-transform infrared

XRD:

X-ray powder diffraction

PSA:

Particle size analysis

FESEM:

Field emission scanning electron microscopy

EDX:

Energy-dispersive X-ray

DRS:

Diffuse reflectance spectroscopy

SOD:

Superoxide dismutase

CAT:

Catalase

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Acknowledgements

This project was financially supported by the Mashhad University of Medical Sciences and the Islamic Azad University of Neyshabur. This study is the result of a research project and thesis presented by Mrs. Pegah Mahmoodi.

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Not applicable.

Author information

Authors and Affiliations

  1. Department of Biochemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran

    Pegah Mahmoodi & Jamshid Mehrzad

  2. Department of Chemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran

    Alireza Motavalizadehkakhky

  3. Advanced Research Center for Chemistry, Biochemistry and Nanomaterial, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran

    Alireza Motavalizadehkakhky, Jamshid Mehrzad & Rahele Zhiani

  4. Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran

    Majid Darroudi

  5. New Materials Technology and Processing Research Center, Department of Chemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran

    Rahele Zhiani

Authors
  1. Pegah Mahmoodi
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  2. Alireza Motavalizadehkakhky
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  3. Majid Darroudi
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  4. Jamshid Mehrzad
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Contributions

PM: investigation, methodology, software, writing—original draft, formal analysis. AM: investigation, software, writing—review and editing. MD: supervision, project administration, validation, methodology, writing-review and editing. JM: data curation, writing—review and editing. RZ: data curation, writing—review and editing.

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Correspondence to Alireza Motavalizadehkakhky or Majid Darroudi.

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Mahmoodi, P., Motavalizadehkakhky, A., Darroudi, M. et al. Green synthesis of zinc and nickel dual-doped cerium oxide nanoparticles: antioxidant activity and cytotoxicity effects. Bioprocess Biosyst Eng 46, 1569–1578 (2023). https://doi.org/10.1007/s00449-023-02920-2

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  • DOI: https://doi.org/10.1007/s00449-023-02920-2

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