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Efficient bio-production of glutaric acid by a metabolically engineered Escherichia coli LQ-1 based on a novel nitrogen source feeding strategy

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Abstract

As an important five-carbon platform chemical to synthesize polyesters and polyamides, glutaric acid is widely used in numerous biochemical fields such as consumer goods, textile, and footwear industries. However, the application of glutaric acid is limited by the low yield of its bio-production. In this study, a metabolically engineered Escherichia coli LQ-1 based on 5-aminovalerate (AMV) pathway was used for glutaric acid fed-batch fermentation. Given the significance of nitrogen source in the bio-production of glutaric acid by AMV pathway, a novel nitrogen source feeding strategy feedbacked by real-time physiological parameters was proposed after evaluating the effects of nitrogen source feeding (such as ammonia and ammonium sulfate) on glutaric acid bio-production. Under the proposed nitrogen source feeding strategy, a significantly improved glutaric acid production of 53.7 g L–1 was achieved in a 30 L fed-batch fermentation by the metabolically engineered E. coli LQ-1, which was an improvement of 52.1% over pre-optimization. Additionally, a higher conversion rate of 0.64 mol mol–1 (glutaric acid/glucose) was obtained compared with the previously reported bio-production of glutaric acid with E. coli. These results indicated that the nitrogen source feeding strategy proposed in this study will be useful for achieving the efficient and sustainable bio-based production of glutaric acid.

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Acknowledgements

This work was supported by grants from National Key Research and Development Program of China (2022YFC2106204).

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Authors and Affiliations

  1. College of Biological Science and Medical Engineering, Innovation Center for Textile Science and Technology, Donghua University, Shanghai, 201620, People’s Republic of China

    Wenwen Bi, Chen Chen, Tiantian Wang, Pan Ye & Naiqiang Li

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  1. Wenwen Bi
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  2. Chen Chen
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  3. Tiantian Wang
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  4. Pan Ye
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  5. Naiqiang Li
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Contributions

WB: Investigation, Data curation, Validation, Writing-original draft. CC: Investigation, Visualization, Data curation. TW: Formal analysis, Validation, Writing-review and editing. PY: Validation, Writing-review and editing. NL: Conceptualization, Resources, Writing-review and editing.

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Correspondence to Naiqiang Li.

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Bi, W., Chen, C., Wang, T. et al. Efficient bio-production of glutaric acid by a metabolically engineered Escherichia coli LQ-1 based on a novel nitrogen source feeding strategy. Bioprocess Biosyst Eng 46, 717–725 (2023). https://doi.org/10.1007/s00449-023-02856-7

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  • DOI: https://doi.org/10.1007/s00449-023-02856-7

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