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Immobilization of Actinobacillus succinogenes on nano- and micro-fiber membranes for efficient and robust production of succinic acid

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Abstract

This work aimed to study the efficiency of nano- and micro- fiber membranes in immobilizing Actinobacillus succinogenes CCTCC M2012036 for succinic acid production. Among the four kinds of electrospun nanofiber membranes of cellulose acetate, chitosan, poly(vinyl alcohol) (PVA) and chitosan-PVA, the cellulose acetate nanofiber membrane-immobilized cells performed the best with a succinic acid concentration and yield to be 27.3 ± 3.5 g/L and 70.9 ± 5.8%. The cell-immobilized viscose microfiber membrane presented good reuse stability, and 17 batches of fermentation without activity loss were realized with the highest succinic acid yield of 83.20%. A microfiber membrane bioreactor was further constructed with the cell-immobilized viscose microfiber membrane to perform fermentation on a larger scale, and the concentration, yield and productivity of succinic acid were 73.20 g/L, 86.50% and 1.49 g/(L⋅h) using a fed-batch strategy, which were 124.30%, 127.60% and 124.2% of those obtained in the traditional fermenter. This study provided an approach for improving the practicality of biological succinic acid production.

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Acknowledgements

The authors are grateful to the financial support from the Open Project of Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University (Grant No. KLIB-KF202205) and the National Natural Science Foundation of China (Grant No. 21604032).

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    Peng-Cheng Chen, Yao-Dan Zhang, Yu-Wei Sun, Li Yin & Pu Zheng

  2. Zhejiang Light Industrial Products Inspection and Research Institute, Hangzhou, Zhejiang, China

    Xiang-Yu Ye

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Correspondence to Peng-Cheng Chen.

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Chen, PC., Zhang, YD., Ye, XY. et al. Immobilization of Actinobacillus succinogenes on nano- and micro-fiber membranes for efficient and robust production of succinic acid. Bioprocess Biosyst Eng 46, 611–620 (2023). https://doi.org/10.1007/s00449-023-02848-7

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