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New insight into the role of oxygen supply for surfactin production in bench-scale bioreactors using induced surface aeration

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Abstract

Surfactin biosurfactant produced by Bacillus sp. has been studied, because it has enormous potential in several applications in the oil and cosmetics industry. The cultivation conditions for obtaining this bioproduct, however, still require attention, as, for example, parameters related to oxygen supply and consumption. In this study, different volumetric oxygen transfer coefficient (KLa) levels (0–11.56 h−1) were tested in bench-scale bioreactor for surfactin biosurfactant production by Bacillus velezensis H2O-1, using induced surface aeration. While conditions close to anaerobiosis showed insignificant production of surfactin, an intermediated KLa condition (4.24 h−1) generated the best surfactin concentration (579.6 mg L−1), with a volumetric productivity of 11.9 mg L−1 h−1. These results showed that the oxygen demand to produce surfactin is not high, being possible to use induced surface aeration strategy in bioreactors, minimizing foam formation. In addition, in all KLa conditions tested, surfactin homologues C14 and C15 had higher relative abundance. Nevertheless, the KLa parameter seems to have had minimal influence on affecting the relative abundances of surfactin homologues produced. Particularly noteworthy in this study is the possibility of producing surfactin using a low-cost and scale-up feasible aeration strategy, unlike the foam collection strategies developed in other studies to obtain this bioproduct.

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Data Availability

The data that support the findings of this study are available from the corresponding author, upon reasonable request.

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Authors and Affiliations

  1. Centro de Tecnologia, Universidade Federal Do Rio de Janeiro, Cidade Universitária, Bl. A, Sl. 549, Ilha Do Fundão, Rio de Janeiro, RJ, 21949-900, Brazil

    Rui de Paula Vieira de Castro, Vanessa Alves Lima Rocha, Maria Eduarda Cezar Fernandes da Silva, Rodrigo Volcan Almeida & Denise Maria Guimarães Freire

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de Paula Vieira de Castro, R., Alves Lima Rocha, V., Cezar Fernandes da Silva, M. et al. New insight into the role of oxygen supply for surfactin production in bench-scale bioreactors using induced surface aeration. Bioprocess Biosyst Eng 45, 2031–2041 (2022). https://doi.org/10.1007/s00449-022-02807-8

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