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Curdlan production from cassava starch hydrolysates by Agrobacterium sp. DH-2

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Abstract

Curdlan is an edible microbial polysaccharide and can be used in food, biomedical and biomaterial fields. To reduce the cost of curdlan production, this study investigated the suitability of cassava starch hydrolysates as carbon source for curdlan production. Cassava starch was hydrolyzed into maltose syrup using β-amylase and pullulanase at various enzyme dosages, temperature, time and addition order of two enzymes. The maltose yield of 53.17% was achieved at starch loading 30% by simultaneous addition β-amylase 210 U/g starch and pullulanase 3 U/g starch at 60 °C for 9 h. Cassava starch hydrolysates were used as carbon source for curdlan production by Agrobacterium sp. DH-2. The curdlan production reached 28.4 g/L with the yield of 0.79 g/g consumed sugar and molecular weight of 1.26 × 106 Da at 96 h with cassava starch hydrolysate at 90 g/L initial sugar concentration. Curdlan produced from cassava starch hydrolysates was characterized using FT-IR spectra and thermo gravimetric analysis. This work indicated that cassava starch was a potential renewable feedstock for curdlan production.

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Funding

This work was financially supported by the Taixing Dongsheng Biotechnology Co. Ltd., China.

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Authors and Affiliations

  1. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China

    Jie Wan, Zhiyu Shao & Xuexia Yang

  2. School of Life Sciences, East China Normal University, Shanghai, 200241, China

    Deming Jiang & Hongliang Gao

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  1. Jie Wan
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  2. Zhiyu Shao
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  3. Deming Jiang
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  4. Hongliang Gao
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Wan, J., Shao, Z., Jiang, D. et al. Curdlan production from cassava starch hydrolysates by Agrobacterium sp. DH-2. Bioprocess Biosyst Eng 45, 969–979 (2022). https://doi.org/10.1007/s00449-022-02718-8

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