Skip to main content
SpringerLink
Log in

Rapid screening of microalgae by a 96-hole air-flowing device

  • Research Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

In this study, a 96-hole air-flowing device (96HAFD) was established for high-throughput screening of three mutant Chlorella strains under air aeration. 96HAFD was first tested for the confirmation of homogeneous air aeration cultivation environment at 1.2 L min−1 for algal screening based on the results of t test (p < 0.05) in the verification of consistency experiment. Then the data of dynamic growth characteristics of three mutant Chlorella strains indicated the good agreement in three screening devices including 96HAFD, flask and tube air-flowing cultivation devices by linear regression analysis between the 96HAFD and tube (R2 = 0.9904), 96HAFD and flask (R2 = 0.9904). At last, the 96HAFD was verified more efficient and reliable in fast screening single algal colony strains when compared with flask and tube air-flowing cultivation devices, because 96HAFD was confirmed have better performances in adaptation to the aeration cultivation circumstance and growing faster in a short period, in addition, 96HAFD had the less percentage of water loss per day (0.11%) than that of flask aeration device (2–3%) and tube aeration device (5–6.5%), which reduced negative effect caused by the water evaporation in the aeration cultivation to make the whole growing system more stable.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Anandarajah K, Mahendraperumal G, Sommerfeld M, Hu Q (2012) Characterization of microalga Nannochloropsis sp. mutants for improved production of biofuels. Appl Energy 96:371–377

    Article  CAS  Google Scholar 

  2. Catalado DA, Hanson M, Schrader LE, Yong VL (1975) Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Commun soil Sci Plant Anal 6(1):71–80

    Article  Google Scholar 

  3. Cheng J, Huang Y, Feng J, Sun J, Zhou JH, Cen KF (2013) Mutate Chlorella sp. by nuclear irradiation to fix high concentrations of CO2. Biores Technol 136:496–501

    Article  CAS  Google Scholar 

  4. De Morais MG, Costa JAV (2007) Isolation and selection of microalgae from coal fired thermoelectric power plant for biofixation of carbon dioxide. Energy Convers Manage 41:633–646

    Google Scholar 

  5. Guillard RRL (1973) Methods for microflagellates and nanoplankton. Handbook of phycological methods. Cambridge University Press, Cambridge UK, pp 80–81

    Google Scholar 

  6. Han W, Li CY, Miao XL (2012) A novel miniature culture system to screen CO2-sequestering microalgae. Energies 5:4372–4389

    Article  CAS  Google Scholar 

  7. Heo J, Cho DH, Ramanan R (2015) PhotoBiobox: a tablet sized, low-cost, high throughput photobioreactor for microalgal screening and culture optimization for growth, lipid content and CO2 sequestration. Biochem Eng J 103:193–197

    Article  CAS  Google Scholar 

  8. Hoshida H, Ohira T, Minematsu A, Akada R, Nishizawa Y (2005) Accumulation of eicosapentaenoic acid in Nannochloropsis sp. in response to elevated CO2 concentrations. J Appl Phycol 17:29–34

    Article  Google Scholar 

  9. Huang GH, Chen F, Kuang YL, He H, Qin A (2016) Current techniques of growing algae using flue gas from exhaust gas industry: a review. Appl Biochem Biotechnol 178:1220–1238

    Article  CAS  Google Scholar 

  10. Huang GH, Li T, Chen F, He H, Kuang YL (2016) High concentration CO2 sequestration by using microalgae in staged cultivation. Environ Prog Sustain Energy 35(6):1862–1867

    Article  CAS  Google Scholar 

  11. Kao CY, Chiu SY, Huang TT, Dai L, Hsu LK, Lin CS (2012) ability of a mutant strain of the microalga Chlorella sp. to capture carbon dioxide for biogas upgrading. Appl Energy 93:176–183

    Article  CAS  Google Scholar 

  12. Lee Y, Tay HS (1991) High CO2 partial pressure depresses productivity and bioenergetics growth yield of Chlorella pyrenoidosa culture. J Appl Phycol 3:95–101

    Article  Google Scholar 

  13. Lukavsky J (1992) the evaluation of algal growth-potential (AGP) and toxicity of water by miniaturized growth bioassay. Water Res 26:1409–1413

    Article  CAS  Google Scholar 

  14. Ma YB, Wang ZY, Zhu M, Yu CJ, Cao YP, Zhang DY, Zhou GK (2013) Increased lipid productivity and TAG content in Nannochloropsis by heavy-ion irradiation mutagenesis. Biores Technol 136:360–367

    Article  CAS  Google Scholar 

  15. Negoro M, Shioji N, Miyamoto K (1991) Growth of microalgae in high CO2 gas and effects of SOX and NOX. Appl Biochem Biotechnol 28–9:877–886

    Article  Google Scholar 

  16. Qi F, Pei H, Hu W, Mu R, Zhang S (2016) Characterization of a microalgal mutant for CO2 biofixation and biofuel production. Energy Convers Manage 122:344–349

    Article  CAS  Google Scholar 

  17. Qi F, Wu DJ, Mu RM, Zhang S, Xu XY (2018) Characterization of a microalgal Uv mutant for CO2 biofixation and biomass production. Biomed Res Int 2018:1–8

    Google Scholar 

  18. Rodolfi L, Zittelli GC, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR (2009) Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng 102:100–112

    Article  CAS  Google Scholar 

  19. Rojickova R, Dvorakova D, Marsalek B (1998) The use of miniaturized algal bioassays in comparison to the standard flask assay. Environ Toxicol Water Qual 13:235–241

    Article  CAS  Google Scholar 

  20. Singh SP, Singh P (2014) Effect of CO2 concentration on algal growth: a review. Renew Sustain Energy Rev 38:172–179

    Article  CAS  Google Scholar 

  21. Song MM, Pei HY, Hu WR, Ma GX (2013) Evaluation of the potential of 10 microalgal strains for biodiesel production. Biores Technol 141:245–251

    Article  CAS  Google Scholar 

  22. Sung K-D, Lee J-S, Shin C-S (1999) CO2 fixation by Chlorella sp. KR-1 and its cultural characteristics. Bioresour Technol 68:269–273

    Article  CAS  Google Scholar 

  23. Wang WL, Wei TT, Fan JH, Yi J, Li YG, Wan MX, Wang J, Bai WM (2018) Repeated mutagenic effects of 60CO-γ irradiation coupled with high-throughput screening improves lipid accumulation in mutant strains of the microalgae Chlorella pyrenoidasa as a feedstock for bioenergy. Algal Res 33:71–77

    Article  CAS  Google Scholar 

  24. Wobbe L, Remacle C (2014) Improving the sunlight-to-biomass conversion efficiency in microalgal biofactories. J Biotechnol 201:28–42

    Article  Google Scholar 

  25. Xu Z, Wang YJ, Chen YC, Spalding MH, Dong L (2017) Microfluidic chip for automated screening of carbon dioxide conditions for automated screening of carbon dioxide conditions for microalgal cell growth. Biomicrofluidics 11(6):064104

    Article  Google Scholar 

  26. Yue L, Chen W (2005) Isolation and determination of cultural characteristics of a new highly CO2 tolerant fresh water microalgae. Energy Convers Manage 46:1868–1876

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This project was financially supported by the Fundamental Research Funds for the Central Universities (2015XKMS041)

Funding

Fundamental Research Funds for the Central Universities, 2015XKMS041.

Author information

Authors and Affiliations

  1. Key Laboratory of Coal Processing and Efficient Utilization of Ministry of Education, China University of Mining and Technology, XuZhou, 221116, China

    Guanhua Huang, Yanyan Han, Wei Li, Zhen Xue, Dengling He & Huan He

Authors
  1. Guanhua Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yanyan Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhen Xue
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dengling He
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Huan He
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Huang G H designed research; Han YY performed research. Huang and Han contributed reagents and analytic tools; Huang and Han analyzed data, Huang wrote the paper; All authors read and approved the manuscript. Authors agreement to authorship and submission of the manuscript for peer review.

Corresponding author

Correspondence to Guanhua Huang.

Ethics declarations

Conflict of interest

The authors declare that they have no competing interest.

Informed consent, human/animal rights

No conflicts, informed consent, human or animal rights applicable.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Huang, G., Han, Y., Li, W. et al. Rapid screening of microalgae by a 96-hole air-flowing device. Bioprocess Biosyst Eng 45, 943–953 (2022). https://doi.org/10.1007/s00449-022-02714-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-022-02714-y

Keywords

Search

Navigation