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Enhanced aerobic granular sludge formation by applying Phanerochaete chrysosporium pellets as induced nucleus

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Abstract

The long start-up period is a major challenging issue for the widespread application of aerobic granular sludge (AGS). In this study, a novel rapid start-up strategy was developed by inoculating Phanerochaete chrysosporium (P. chrysosporium) pellets as the induced nucleus in a sequencing batch airlift reactor (SBAR) to enhance activated sludge granulation. The results demonstrated that P. chrysosporium pellets could effectively shorten the aerobic granulation time from 32 to 20 days. The AGS promoted by P. chrysosporium pellets had a larger average diameter (2.60–2.74 mm) than that without P. chrysosporium pellets (1.78–1.88 mm) and had better biomass retention capacity and sedimentation properties; its mixed liquor suspended solids (MLSS) and sludge volume index (SVI30) reached approximately 5.2 g/L and 45 mL/g, respectively. The addition of P. chrysosporium pellets promoted the secretion of extracellular polymeric substances (EPS), especially protein (PN). The removal efficiencies of chemical oxygen demand (COD), ammonia nitrogen (NH4+-N), total nitrogen (TN), and total phosphorus (TP) in P. chrysosporium pellets reactor were 98.91%, 89.17%, 64.73%, and 94.42%, respectively, which were higher than those in the reactor without P. chrysosporium pellets (88.73%, 82.09%, 55.75%, and 88.92%). High throughput sequencing analysis indicated that several functional genera that were responsible for the formation of aerobic granules and the removal of pollutants, such as Acinetobacter, Pseudomonas, Janthinobacterium, and Enterobacter, were found to be predominant in the mature sludge granules promoted by P. chrysosporium pellets.

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Data availability

The datasets used or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This study was supported by the China Major Science and Technology Program for Water Pollution Control and Treatment (No. 2018ZX07601-002), the Liaoning Doctoral Research Start-up Fund (No.2019-BS-087) and Shenyang Science and Technology Plan Fund Project (No. 21-108-9-25).

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  1. Key Laboratory of the Ministry of Education for Eco-Restoration of Regional Contaminated Environment, Shenyang University, Shenyang, 110044, Liaoning, China

    Yihua Dong, Feng Chen, Zhiwen Yin & Xueying Zhang

  2. School of Resources and Civil Engineering, Northeastern University, Shenyang, 110819, Liaoning, China

    Liang Li

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D Y-H: conceptualization, funding acquisition, investigation, visualization, methodology, writing-review & editing. C F: investigation, writing-original draft. L L: conceptualization, supervision, review & editing, funding acquisition. Y Z-W: investigation, writing-original draft. Z X–Y: investigation, writing-review & editing. All authors read and approved the final manuscript.

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Dong, Y., Chen, F., Li, L. et al. Enhanced aerobic granular sludge formation by applying Phanerochaete chrysosporium pellets as induced nucleus. Bioprocess Biosyst Eng 45, 815–828 (2022). https://doi.org/10.1007/s00449-022-02698-9

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