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An eco-friendly biocatalytic process for producing better quality paper from sugarcane bagasse using ultrafiltered enzymes concoction

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Abstract

In the current study, pretreatment of sugarcane bagasse has been carried out with ultrafiltered xylano-pectinolytic enzymes, before conventional chemical bleaching process. Optimized enzymatic dose (4 IU xylanase and 1.2 IU pectinase per g of oven dried pulp) and retention time (180 min) were determined on the basis of maximum decrement in kappa number (from 20.93 to 15.32), release of maximum sugars (7.4 mg/g) as well as attainment of maximum brightness (25.1% ISO), whiteness (from − 57.3 to − 41.9) and minimum yellowness (from 48.7 to 35.3) of the pulp samples. Enzymatically treated samples also showed release of phenolic, lignin and hydrophobic compounds in their filtrates. Nearly 30% decrement in the exhaustion of bleaching chemical dose was detected as compared to control samples. The physical properties such as tear index, burst index, double fold number, breaking length, gurley porosity and viscosity of enzymo-chemically treated bagasse pulp samples were improved by 6.68%, 33.86%, 22.92%, 13.43%, 17.5% and 9.64%, respectively. Additionally, a decrement of 36.75% and 28.29% in the values of BOD and COD of the effluents was also noted, which demonstrated the fact that, inclusion of enzymes in chemical based protocols of paper and pulp industries could be a highly beneficial and eco-friendly approach in upcoming decades. This is the first report mentioning the effect of ultrafiltered xylano-pectinolytic enzymes concoction on sugarcane bagasse pulp.

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Funding

The financial support was provided by Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India (Grant Number: BT/PR 20438/BCE/8/1220/2016 for 3 years).

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Authors and Affiliations

  1. Department of Biotechnology, Kurukshetra University, Kurukshetra, India

    Sharad Agrawal, Raksha Nagpal & Ritu Mahajan

  2. Department of Life Sciences, Sharda University, Greater Noida, India

    Sharad Agrawal

  3. Department of Life Sciences, IAMR College, Ghaziabad, India

    Divya Sharma

  4. Avantha Centre for Industrial Research and Development, Paper Mill Campus, Yamuna Nagar, India

    Nishikant Bhardwaj

Authors
  1. Sharad Agrawal
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  2. Raksha Nagpal
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  3. Divya Sharma
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  4. Nishikant Bhardwaj
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  5. Ritu Mahajan
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Contributions

RM (corresponding author) given the idea of concept, planning and designing of various experiments done for this manuscript. SA (first author) done all experimental work mentioned in this manuscript at Research laboratory level. RN: done the experimental work mentioned in this manuscript at Paper Industry level. DS: analysis of effluent characteristics done. NKB supervised the research work done at Paper Industry level.

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Correspondence to Ritu Mahajan.

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Agrawal, S., Nagpal, R., Sharma, D. et al. An eco-friendly biocatalytic process for producing better quality paper from sugarcane bagasse using ultrafiltered enzymes concoction. Bioprocess Biosyst Eng 45, 741–747 (2022). https://doi.org/10.1007/s00449-022-02695-y

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  • DOI: https://doi.org/10.1007/s00449-022-02695-y

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