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Improved catalytic performance of carrier-free immobilized lipase by advanced cross-linked enzyme aggregates technology

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Abstract

The cross-linked enzyme aggregates (CLEAs) are one of the technologies that quickly immobilize the enzyme without a carrier. In this study, ionic liquid with amino group (1-aminopropyl-3-methylimidazole bromide, FIL) was used as the novel functional surface molecule to modify CRL (Candida rugosa lipase, CRL). The enzymatic properties of CRL-FIL-CLEAs were investigated. The activity of CRL-FIL-CLEAs (5.51 U/mg protein) was 1.9 times higher than that of CRL-CLEAs (2.86 U/mg protein) without surface modification. After incubating in a centrifuge tube for 50 min at 60 °C, CRL-FIL-CLEAs still maintained 61% of its initial activity, while the value for CRL-CLEAs was only 22%. After repeated use for five times, compared with the 22% residual activity of CRL-CLEAs, the value of CRL-FIL-CLEAs was 51%. Based on the above results, it was indicated that this method provided a new idea for the effective synthesis of immobilized enzyme.

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Acknowledgements

The work was funded by the National Natural Science Foundation of China (No. 21406093), Natural Science Foundation of Jiangsu Province (BK20140529), Research Foundation on full coverage inspection system of University Laboratory (GS2019YB07), Open Project Program of State Key Laboratory of Food Science and Technology of Jiangnan University (SKLF-KF-201919), Key University Science Research Project of Jiangsu Province (14KJB530001), China Postdoctoral Science Foundation (2014M550271), and Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Xia Jiaojiao & Zou Bin

  2. School of Food and Biological Engineering, Jiangsu University, No. 301 Xuefu Road, Zhenjiang, 212013, China

    Xia Jiaojiao, Yan Yan, Zou Bin & Liu Feng

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  1. Xia Jiaojiao
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  2. Yan Yan
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Contributions

Xia Jiaojiao: investigation and writing—original draft. Yan Yan: conceptualization, methodology, and formal analysis. Zou Bin: conceptualization, supervision, resources, and writing—review and editing. Liu Feng: investigation, resources, and writing—review and editing

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Correspondence to Zou Bin.

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Jiaojiao, X., Yan, Y., Bin, Z. et al. Improved catalytic performance of carrier-free immobilized lipase by advanced cross-linked enzyme aggregates technology. Bioprocess Biosyst Eng 45, 147–158 (2022). https://doi.org/10.1007/s00449-021-02648-x

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