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Green Roccella phycopsis Ach. mediated silver nanoparticles: synthesis, characterization, phenolic content, antioxidant, antibacterial and anti-acetylcholinesterase capacities

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Abstract

In this study, we develop here for the first time an easy, eco-friendly method for synthesizing silver nanoparticles (AgNPs) using the lichen Roccella phycopsis. AgNPs formation was first determined by a color change of the lichen filtrate to brown, subsequent to addition of AgNO3 solution, and confirmed by a maximum absorbance peak at 425 nm in UV–vis spectrum. Scanning electron microscope images showed a spherical shape with a size distribution between 11 and 18 nm, while the elemental composition was elucidated by the energy dispersive X-ray spectroscopy. The chemical compounds responsible for reduction and stabilization of silver nanoparticles were detected by Frourier transform infrared spectroscopy analysis. The synthesized R. phycopsis silver nanoparticles displayed a strong antioxidant activity. Further, the antibacterial activity was more effective against Gram-negative than Gram-positive bacteria. Besides, the R. phycopsis-AgNPs were potent in inhibiting acetylcholinesterase enzyme with IC50 value of 1.65 ± 0.07 mg/mL.

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Acknowledgements

We are thankful to the Unity of Research for Vegetal Ecology at the University of El-Manar II, Faculty of Sciences for providing lab facilities. We are also thankful to the Tunisian National Oil Company (ETAP) for assistance for SEM.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Faculty of Sciences, Plant, Soil, Environment Interactions Laboratory, University of El-Manar II, Campus Academia, 2092, Tunis, Tunisia

    Mariem Ben Salah & Ayda Khadhri

  2. Laboratory of Epidemiology and Microbiology Veterinary Bacteriology Groups and Biotechnology Development, Pasteur Institute of Tunis, El Manar University, Tunis, Tunisia

    Chedia Aouadhi

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  1. Mariem Ben Salah
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  2. Chedia Aouadhi
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Contributions

MBS: conceptualization, methodology, formal analysis, investigation, interpretation, data curation, writing. CA: carried out the antibacterial activity experiments. AK: methodology, validation, resources, supervision, review and editing.

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Correspondence to Ayda Khadhri.

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Ben Salah, M., Aouadhi, C. & Khadhri, A. Green Roccella phycopsis Ach. mediated silver nanoparticles: synthesis, characterization, phenolic content, antioxidant, antibacterial and anti-acetylcholinesterase capacities. Bioprocess Biosyst Eng 44, 2257–2268 (2021). https://doi.org/10.1007/s00449-021-02601-y

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