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Evaluation of gamma-aminobutyric acid (GABA) production by Lactobacillus plantarum using two-step fermentation

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Abstract

Lactic acid bacteria (Lactobacillus plantarum KCTC 3103) were fermented to produce gamma-aminobutyric acid (GABA). The conditions of the modified synthetic medium were optimized as 5 g/L glucose, 10 g/L yeast extract, 100 g/L rice bran extract, and 1.0 g/L ascorbic acid for GABA production. Single-step fermentation of cell growth and GABA production with a modified synthetic medium was higher than those with an MRS medium. Two-step fermentation was evaluated by separating the cell growth and GABA production under a modified synthetic medium. The cell concentration of 1.65 g dcw/L produced by the modified synthetic medium was higher than that of 1.0 g dcw/L produced by the MRS medium at 36 h from the first step of two-step fermentation. The highest GABA production of L. plantarum KCTC 3103 was 0.67 g/L with monosodium glutamate addition at 60 h in the second step of fermentation. Two-step fermentation with the modified synthetic medium is suitable for GABA production because of its high GABA productivity and favorable cell growth.

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Acknowledgements

This work was supported by a research grant from Hankyong National University in the year of 2018.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, College of Engineering, Global K-Food Research Center, Hankyong National University, Anseong-Si, 17579, Republic of Korea

    Na Yeon Kim & Chae Hun Ra

  2. Department of Biotechnology, Pukyong National University, Busan, 48513, Republic of Korea

    Sung-Koo Kim

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  1. Na Yeon Kim
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  2. Sung-Koo Kim
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Correspondence to Chae Hun Ra.

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Kim, N.Y., Kim, SK. & Ra, C.H. Evaluation of gamma-aminobutyric acid (GABA) production by Lactobacillus plantarum using two-step fermentation. Bioprocess Biosyst Eng 44, 2099–2108 (2021). https://doi.org/10.1007/s00449-021-02586-8

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