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Coproduction of polymalic acid and liamocins from two waste by-products from the xylitol and gluconate industries by Aureobasidium pullulans

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Abstract

The coproduction of polymalic acid (PMA) and liamocins, two important metabolites secreted by Aureobasidium pullulans, from two waste by-products from the xylitol and gluconate industries was investigated in shake flasks and fermentors, confirming that waste xylose mother liquor (WXML) could be utilized as an economical feedstock without any pretreatment. Gluconate could strengthen carbon flux and NADPH supply for the synergetic biosynthesis of PMA and liamocins. High PMA and liamocin titers of 82.9 ± 2.1 and 28.3 ± 2.7 g/L, respectively, were obtained from the coupled WXML and waste gluconate mother liquor (WGML) in batch fermentation, with yields of 0.84 and 0.25 g/g, respectively. These results are comparable to those obtained from renewable feedstocks. Economic assessment of the process revealed that PMA and liamocins could be coproduced from two by-products at costs of $1.48/kg or $0.67/kg (with liamocins credit), offering an economic and sustainable process for the application of waste by-products.

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Acknowledgements

This study was supported in part by grants from the National Natural Science Foundation of China (Grant No. 31871783 and 31571816), Fundamental Research Funds for the Central Universities (XDJK2018AC002), Chongqing Social and People's Livelihood Guarantee Special Program (cstc2016shmszx80075), and National High Technology Research and Development Program of China (863 Program) (2015AA021005).

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Authors and Affiliations

  1. College of Pharmaceutical Sciences, Southwest University, 2 Tian Sheng Road, Beibei, Chongqing, 400715, People’s Republic of China

    Tianfu Li, Wenwen Yang, Xingran Xu & Xiang Zou

  2. School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Guanggu 1st road, Wuhan, Hubei Province, 430205, People’s Republic of China

    Jie Chen

  3. Auhui Sealong Biotechnology Co., Ltd, 6 Jinchong Road, Bengbu, 233316, Anhui Province, People’s Republic of China

    Yan Zhang

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  1. Tianfu Li
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Li, T., Yang, W., Xu, X. et al. Coproduction of polymalic acid and liamocins from two waste by-products from the xylitol and gluconate industries by Aureobasidium pullulans. Bioprocess Biosyst Eng 44, 1965–1974 (2021). https://doi.org/10.1007/s00449-021-02578-8

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