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Nitrogen removal performance, quantitative detection and potential application of a novel aerobic denitrifying strain, Pseudomonas sp. GZWN4 isolated from aquaculture water

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Abstract

A novel Pseudomonas sp. GZWN4 with the aerobic nitrogen removal ability was isolated from aquaculture water, whose removal efficiency of NO2−–N, NO3−–N and NH4+–N was 99.72%, 82.54% and 98.62%, respectively. The key genes involved in nitrogen removal, nxr, napA, narI, nirS, norB and nosZ, were successfully amplified and by combination with the results of nitrogen balance analysis, it was inferred that the denitrification pathway of strain GZWN4 was NO3−–N → NO2−–N → NO → N2O → N2. The strain GZWN4 had excellent nitrite removal performance at pH 7.0–8.5, temperature 25–30 ℃, C/N ratio 5–20, salinity 8–32‰ and dissolved oxygen concentration 2.52–5.73 mg L−1. The receivable linear correlation (R2 = 0.9809) was obtained with the range of quantification between l03 and 108 CFU mL−1 of the strain by enzyme-linked immunosorbent assay. Strain GZWN4 could maintain high abundance in the actual water and wastewater of mariculture and the removal efficiency of TN were 52.57% and 63.64%, respectively. The safety evaluation experiment showed that the strain GZWN4 had no hemolysis and high biosecurity toward shrimp Litopenaeus vannamei. The excellent nitrogen removal ability and adaptability to aquaculture environment made strain GZWN4 a promising candidate for treatment of water and wastewater in aquaculture.

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Acknowledgements

This work was supported by project of Bio-Form biotechnology (Shandong) Co. LTD (20160359).

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Authors and Affiliations

  1. The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Yushan Road 5, Qingdao, 266003, Shandong, China

    Zhaopeng Su, Yun Li, Luqing Pan, Ziyan He,  Le Dou, Liping Liu & Mengyu Zhang

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Contributions

All the authors contributed to the study conception and design. All the authors read and approved the final manuscript. ZS: conceptualization, methodology, investigation, formal analysis, writing—original draft and project administration; YL: supervision, conceptualization, and writing—review and editing; LP: investigation and formal analysis; ZH: investigation; LD: investigation; LL: investigation; MZ: investigation.

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Correspondence to Yun Li.

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Su, Z., Li, Y., Pan, L. et al. Nitrogen removal performance, quantitative detection and potential application of a novel aerobic denitrifying strain, Pseudomonas sp. GZWN4 isolated from aquaculture water. Bioprocess Biosyst Eng 44, 1237–1251 (2021). https://doi.org/10.1007/s00449-021-02523-9

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