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Waste soybean frying oil for the production, extraction, and characterization of cell-wall-associated lipases from Yarrowia lipolytica

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Abstract

The lipolytic yeast Yarrowia lipolytica produces cell-wall-associated lipases, namely Lip7p and Lip8p, that could have interesting properties as catalyst either in free (released lipase fraction—RLF) or cell-associated (cell-bound lipase fraction—CBLF) forms. Herein, a mixture of waste soybean frying oil, yeast extract and bactopeptone was found to favor the enzyme production. Best parameters for lipase activation and release from the cell wall by means of acoustic wave treatment were defined as: 26 W/cm2 for 1 min for CBLF and 52 W/cm2 for 2 min for RLF. Optimal pH and temperature values for lipase activity together with storage conditions were similar for both the free enzyme and cell-associated one: pH 7.0; T = 37 °C; and > 70% residual activity for 60 days at 4, − 4 °C and for 15 days at 30 °C.

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Funding

The financial support of CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Ministry of Education, Brazil) and WBI (Wallonie Bruxelles International, Belgium, Grant no. 154157) is greatly acknowledged. P. Amaral received a research fellowship from FAPERJ (Fundação Carlos Chagas Filho de Amparo à Pesquisa no Estado do Rio de Janeiro) (Grant number E-26/202.870/2015 BOLSA) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) (Grant number 308626/2019–2).

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Authors and Affiliations

  1. Escola de Química, Universidade Federal Do Rio de Janeiro, Av. Athos da Silveira Ramos, 149—CT, Bl. E, Ilha Do Fundão, Rio de Janeiro, RJ, 21941-909, Brazil

    Patrícia M. B. Nunes, Jully L. Fraga, Rafael B. Ratier, Maria Helena M. Rocha-Leão & Priscilla F. F. Amaral

  2. Embrapa Agroindústria Tropical, Rua Doutora Sara Mesquita, 2270, Pici, Fortaleza, CE, 60511-110, Brazil

    Ana I. S. Brígida

  3. Microbial Processes and Interactions, Terra Teaching and Research Centre, University of Liège—Gembloux Agro-Bio Tech, Av. de la Faculté 2B, 5030, Gelmbloux, Belgium

    Patrícia M. B. Nunes & Patrick Fickers

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  1. Patrícia M. B. Nunes
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  2. Jully L. Fraga
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  3. Rafael B. Ratier
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  4. Maria Helena M. Rocha-Leão
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  5. Ana I. S. Brígida
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  7. Priscilla F. F. Amaral
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Contributions

Conceptualization: PFFA, PF; data curation: PFFA; formal analysis: PMBN; funding acquisition: PFFA, PF; investigation: RBR, PMBN, JLF; methodology: JLF, PFFA, AISB, PF; project administration: PFFA, PF; resources: PFFA, PF; software: JLF, PFFA; supervision: PFFA, AISB, MHM-L, PF; validation: PMBN; visualization: PMBN, PFFA; roles/writing—original draft: PMBN; writing—review & editing: PFFA, PF.

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Correspondence to Priscilla F. F. Amaral.

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All authors declare no competing/ conflicts of interests. The funders had no decision on the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

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Nunes, P.M.B., Fraga, J.L., Ratier, R.B. et al. Waste soybean frying oil for the production, extraction, and characterization of cell-wall-associated lipases from Yarrowia lipolytica. Bioprocess Biosyst Eng 44, 809–818 (2021). https://doi.org/10.1007/s00449-020-02489-0

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