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Preparation and synthetic dye decolorization ability of magnetic cross-linked enzyme aggregates of laccase from Bacillus amyloliquefaciens

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Abstract

Laccases are versatile oxidases that are capable of decolorizing various synthetic dyes. Recombinant Bacillus amyloliquefaciens laccase was immobilized as magnetic cross-linked enzyme aggregates (M-CLEAs) for application in dye decolorization. Several parameters influencing the activity recovery were evaluated during the synthesis of M-CLEAs. With ammonium sulfate as precipitant, maximum activity was recovered by cross-linking with 0.16% glutaraldehyde for 1 h. The prepared M-CLEAs exhibited improved activity under alkaline conditions. It remained 74% activity after incubation at 60 °C for 5 h. Enhanced tolerance towards NaCl was also observed for the M-CLEAs, with 68% activity remaining in the presence of 1 M NaCl. The immobilized laccase could rapidly decolorize more than 93% of reactive black 5 and indigo carmine in 1 h, while its catalytic efficiency towards reactive blue 19 was relatively low. After four cycles of consecutive reuse, the M-CLEAs could decolorize 92% of indigo carmine. The easy recovery and reusability of M-CLEAs facilitate the potential application of bacterial laccase in dye decolorization.

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References

  1. Holkar CR, Jadhav AJ, Pinjari DV, Mahamuni NM, Pandit AB (2016) A critical review on textile wastewater treatments: possible approaches. J Environ Manage 182:351–366

    Article  CAS  Google Scholar 

  2. Tkaczyk A, Mitrowska K, Posyniak A (2020) Synthetic organic dyes as contaminants of the aquatic environment and their implications for ecosystems: a review. Sci Total Environ 717:137222

    Article  CAS  Google Scholar 

  3. Vikrant K, Giri BS, Raza N, Roy K, Kim KH, Rai BN, Singh RS (2018) Recent advancements in bioremediation of dye: current status and challenges. Bioresour Technol 253:355–367

    Article  CAS  Google Scholar 

  4. Deska M, Kończak B (2019) Immobilized fungal laccase as “green catalyst” for the decolourization process-State of the art. Process Biochem 84:112–123

    Article  CAS  Google Scholar 

  5. Debnath R, Saha T (2020) An insight into the production strategies and applications of the ligninolytic enzyme laccase from bacteria and fungi. Biocatal Agric Biotechnol 26:101645

    Article  Google Scholar 

  6. Wang M, Qi W, Su R, He Z (2015) Advances in carrier-bound and carrier-free immobilized nanobiocatalysts. Chem Eng Sci 135:21–32

    Article  CAS  Google Scholar 

  7. Sheldon RA (2019) CLEAs, combi-CLEAs and ‘smart’ magnetic CLEAs: biocatalysis in a bio-based economy. Catalysts 9:261

    Article  CAS  Google Scholar 

  8. Talekar S, Joshi A, Joshi G, Kamat P, Haripurkar R, Kambale S (2013) Parameters in preparation and characterization of cross linked enzyme aggregates (CLEAs). RSC Adv 3:12485–12511

    Article  CAS  Google Scholar 

  9. Kumar VV, Sivanesan S, Cabana H (2014) Magnetic cross-linked laccase aggregates—bioremediation tool for decolorization of distinct classes of recalcitrant dyes. Sci Total Environ 487:830–839

    Article  CAS  Google Scholar 

  10. Kumar VV, Cabana H (2016) Towards high potential magnetic biocatalysts for on-demand elimination of pharmaceuticals. Bioresour Technol 200:81–89

    Article  CAS  Google Scholar 

  11. Yang J, Wang Z, Lin Y, Ng TB, Ye X, Lin J (2017) Immobilized Cerrena sp. laccase: preparation, thermal inactivation, and operational stability in malachite green decolorization. Sci Rep 7:16429

    Article  Google Scholar 

  12. Sadeghzadeh S, Nejad ZG, Ghasemi S, Khafaji M, Borghei SM (2020) Removal of bisphenol A in aqueous solution using magnetic cross-linked laccase aggregates from Trametes hirsuta. Bioresour Technol 306:123169

    Article  CAS  Google Scholar 

  13. Guan ZB, Luo Q, Wang HR, Chen Y, Liao XR (2018) Bacterial laccases: promising biological green tools for industrial applications. Cell Mol Life Sci 75:3569–3592

    Article  CAS  Google Scholar 

  14. Sinirlioglu ZA, Sinirlioglu D, Akbas F (2013) Preparation and characterization of stable cross-linked enzyme aggregates of novel laccase enzyme from Shewanella putrefaciens and using malachite green decolorization. Bioresour Technol 146:807–811

    Article  CAS  Google Scholar 

  15. Ece S, Lambertz C, Fischer R, Commandeur U (2017) Heterologous expression of a Streptomyces cyaneus laccase for biomass modification applications. AMB Express 7:86

    Article  Google Scholar 

  16. Wang J, Lu L, Feng F (2017) Improving the indigo carmine decolorization ability of a Bacillus amyloliquefaciens laccase by site-directed mutagenesis. Catalysts 7:275

    Article  Google Scholar 

  17. Ranjbakhsh E, Bordbar AK, Abbasi M, Khosropour AR, Shams E (2012) Enhancement of stability and catalytic activity of immobilized lipase on silica-coated modified magnetite nanoparticles. Chem Eng J 179:272–276

    Article  CAS  Google Scholar 

  18. Alex D, Mathew A, Sukumaran RK (2014) Esterases immobilized on aminosilane modified magnetic nanoparticles as a catalyst for biotransformation reactions. Bioresour Technol 167:547–550

    Article  CAS  Google Scholar 

  19. Matijošytė I, Arends IWCE, de Vires S, Sheldon RA (2010) Preparation and use of cross-linked enzyme aggregates (CLEAs) of laccases. J Mol Catal B Enzyme 62:142–148

    Article  Google Scholar 

  20. Martins LO, Soares CM, Pereira MM, Teixeira M, Costa T, Jones GH, Henriques AO (2002) Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat. J Biol Chem 277:18849–18859

    Article  CAS  Google Scholar 

  21. Lu L, Wang TN, Xu TF, Wang JY, Wang CL, Zhao M (2013) Cloning and expression of thermos-alkali-stable laccase of Bacillus licheniformis in Pichia pastoris and its characterization. Bioresour Technol 134:81–86

    Article  CAS  Google Scholar 

  22. Jiang Y, Wang Q, He Y, Zhou L, Gao J (2014) Co-aggregation of laccase and nature egg white: a simple method to prepare stable and recyclable biocatalyst. Appl Biochem Biotechnol 172:2496–2506

    Article  CAS  Google Scholar 

  23. Yang J, Xu X, Yang X, Ye X, Lin J (2016) Cross-linked enzyme aggregates of Cerrena laccase: preparation, enhanced NaCl tolerance and decolorization of remazol brilliant blue reactive. J Taiwan Inst Chem Eng 62:1–7

    Article  Google Scholar 

  24. Fathali Z, Rezaei S, Faramarzi MA, Habibi-Rezaei M (2019) Catalytic phenol removal using entrapped cross-linked laccase aggregates. Int J Biol Macromol 122:359–366

    Article  CAS  Google Scholar 

  25. Barbosa O, Ortiz C, Berenguer-Murcia Á, Torres R, Rodrigues RC, Fernandez-Lafuente R (2014) Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization. RSC Adv 4:1583–1600

    Article  CAS  Google Scholar 

  26. Alves NR, Pereira MM, Giordano RLC, Tardioli PW, Lima ÁS, Soares CMF, Souza RL (2020) Design for preparation of more active cross-linked enzyme aggregates of Burkholderia cepacia lipase using palm fiber residue. Bioprocess Biosyst Eng. https://doi.org/10.1007/s00449-020-02419-0

    Article  PubMed  Google Scholar 

  27. Torabizadeh H, Mikani M (2018) Nano-magnetic cross-linked enzyme aggregates of naringinase an efficient nanobiocatalyst for naringin hydrolysis. Int J Biol Macromol 117:134–143

    Article  CAS  Google Scholar 

  28. Bedade DK, Muley AB, Singhal RS (2019) Magnetic cross-linked enzyme aggregates of acrylamidase from Cupriavidus oxalaticus ICTDB921 for biodegradation of acrylamide from industrial waste water. Bioresour Technol 272:137–145

    Article  CAS  Google Scholar 

  29. Shaarani SM, Jahim JM, Rahman RA, Idris A, Murad AMA, Illias RM (2016) Silanized maghemite for cross-linked enzyme aggregates of recombinant xylanase from Trichoderma reesei. J Mol Catal B Enzyme 133:65–76

    Article  CAS  Google Scholar 

  30. Brander S, Mikkelsen JD, Kepp KP (2014) Characterization of an alkali- and halide-resistant laccase expressed in E. coli: CotA from Bacillus clausii. PLoS ONE 9:e99402

    Article  Google Scholar 

  31. Berini F, Verce M, Ausec L, Rosini E, Tonin F, Pollegioni L, Mandić-Mulec I (2018) Isolation and characterization of a heterologously expressed bacterial laccase from the anaerobe Geobacter metallireducens. Appl Microbiol Biotechnol 102:2425–2439

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the Natural Science Foundation of Heilongjiang Province of China (C2017010) and the Fundamental Research Funds for the Central Universities (2572017CA22).

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  1. Lei Lu

    Present address: College of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Harbin, 150040, China

Authors and Affiliations

  1. Key Laboratory of Saline-Alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin, 150040, China

    Hongrao Wang, Xiaoyan Li & Lei Lu

  2. College of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Harbin, 150040, China

    Hongrao Wang, Shuran Han, Jiayi Wang & Xiaoyan Li

  3. College of Water Conservancy and Civil Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China

    Shuyu Yu

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  1. Hongrao Wang
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Wang, H., Han, S., Wang, J. et al. Preparation and synthetic dye decolorization ability of magnetic cross-linked enzyme aggregates of laccase from Bacillus amyloliquefaciens. Bioprocess Biosyst Eng 44, 727–735 (2021). https://doi.org/10.1007/s00449-020-02481-8

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