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Development of a purification process via crystallization of xylitol produced for bioprocess using a hemicellulosic hydrolysate from the cashew apple bagasse as feedstock

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Abstract

Xylitol was biotechnologically produced by Kluyveromyces marxianus ATCC36907 using the hemicellulosic hydrolysate of the cashew apple bagasse (CABHH). Sequentially, the present study investigated the recovery and purification of xylitol evaluating different antisolvents [ethanol, isopropanol and the ionic liquid 2-hydroxyl-ethylammonium acetate (2-HEAA)], their proportion in the medium (10–90% v/v), and their cooling rate (VC 0.25–0.50 °C/min). These processes were contrasted with the crystallization process of commercial xylitol. This study is the first to assess xylitol crystallization using a protic ionic liquid. The hydrolysate obtained from a mild treatment with sulfuric acid contained mainly glucose and xylose at concentrations of 15.7 g/L and 11.9 g/L, respectively. With this bioprocess, a maximum xylitol production of 4.5 g/L was achieved. The performance of the investigated antisolvents was similar in all conditions evaluated in the crystallization process of the commercial xylitol, with no significant difference in yields. For the crystallization processes of the produced xylitol, the best conditions were: 50% (v/v) isopropanol as antisolvent, cooling rate of 0.5 °C/min, with a secondary nucleation of yield and purity of 69.7% and 84.8%, respectively. Under the same linear cooling rate, using ethanol, isopropanol or the protic ionic liquid 2-hydroxyl-ethylammonium acetate (2-HEAA), crystallization did not occur, probably due to the presence of carbohydrates not metabolized by the yeast in the broth, which influences the solubility curve of xylitol. With the results of this work, a possible economical and environmentally friendly process of recovery and purification of xylitol from CABHH could be proposed.

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Acknowledgements

The authors are grateful for the financial support provided by the Brazilian research agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (Grant no. 306949/2018-0),Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES and Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico - FUNCAP (Process DEP-0164-00357.01.00/19), and would like to thank Jandaia Sucos do Brasil S/A for the cashew apple bagasse donated. We also thank the Analytical Central of the Universidade Federal do Ceará for their invaluable help with the SEM analysis, and the Applied Thermodynamics Research Group (GPTA) for the FTIR, and X-ray Laboratory of the Universidade Federal do Ceará by X-ray analyses (CNPq Process: 402561/2007-4—MCT/CNPq nº 10/2007).

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  1. Departament of Chemical Engineering, Federal University of Ceara, Campus do Pici, Bloco 709, Fortaleza, CE, 60455-760, Brazil

    José Edvan Marques Júnior & Maria Valderez Ponte Rocha

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  1. José Edvan Marques Júnior
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Correspondence to Maria Valderez Ponte Rocha.

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Marques Júnior, J.E., Rocha, M.V.P. Development of a purification process via crystallization of xylitol produced for bioprocess using a hemicellulosic hydrolysate from the cashew apple bagasse as feedstock. Bioprocess Biosyst Eng 44, 713–725 (2021). https://doi.org/10.1007/s00449-020-02480-9

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