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Deconstruction of banana peel for carbohydrate fractionation

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Abstract

The deconstruction of banana peel for carbohydrate recovery was performed by sequential treatment (acid, alkaline, and enzymatic). The pretreatment with citric acid promoted the extraction of pectin, resulting in a yield of 8%. In addition, xylose and XOS, 348.5 and 17.3 mg/g xylan, respectively, were also quantified in acidic liquor as a result of partial depolymerization of hemicellulose. The spent solid was pretreated with alkaline solution (NaOH or KOH) for delignification and release of residual carbohydrates from the hemicellulose. The yields of xylose and arabinose (225.2 and 174.0 mg/g hemicellulose) were approximately 40% higher in the pretreatment with KOH, while pretreatment with NaOH promoted higher delignification (67%), XOS yield (32.6 mg/g xylan), and preservation of cellulosic fraction. Finally, the spent alkaline solid, rich in cellulose (76%), was treated enzymatically to release glucose, reaching the final concentration of 28.2 g/L. The mass balance showed that through sequential treatment, 9.9 g of xylose, 0.5 g of XOS, and 8.2 g of glucose were obtained from 100 g of raw banana peels, representing 65.8% and 46.5% conversion of hemicellulose and cellulose, respectively. The study of the fractionation of carbohydrates in banana peel proved to be a useful tool for valorization, mainly of the hemicellulose fraction for the production of XOS and xylose with high value applications in the food industry.

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Acknowledgements

The authors are grateful to CAPES-PRINT, project number 88887.310560/2018-00 and FAPESP, project number 2019/08542-3.

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Authors and Affiliations

  1. Laboratory of Biological Engineering, Department of Chemical and Food Engineering, UFSC – Federal University of Santa Catarina, Florianópolis, SC, 88040-970, Brazil

    Maria Angélica F. Pereira, Carla Roana M. Monteiro, Gabriela N. Pereira, Sidnei E. Bordignon Júnior, Débora de Oliveira & Patrícia Poletto

  2. Yeast Molecular Biology and Biotechnology Laboratory, Department of Biochemistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil

    Eduardo Zanella & Boris U. Stambuk

  3. Laboratory of Bioprocess and Metabolic Engineering, Department of Faculty of Food Engineering, UNICAMP - University of Campinas, Campinas, SP, 13083-862, Brazil

    Patrícia F. Ávila & Rosana Goldbeck

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  1. Maria Angélica F. Pereira
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Pereira, M.A.F., Monteiro, C.R.M., Pereira, G.N. et al. Deconstruction of banana peel for carbohydrate fractionation. Bioprocess Biosyst Eng 44, 297–306 (2021). https://doi.org/10.1007/s00449-020-02442-1

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