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Precise engineering of neutrophil membrane coated with polymeric nanoparticles concurrently absorbing of proinflammatory cytokines and endotoxins for management of sepsis

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Abstract

Sepsis, ensuing from unrestrained inflammatory replies to bacterial infections, endures with high injury and mortality worldwide. Presently, active sepsis management is missing in the hospitals during the surgery, and maintenance remnants mainly helpful. Now, we have constructed the macrophage bio-mimic nanoparticles for the treatment of sepsis and its management. Biomimetic macrophage nanoparticles containing a recyclable polymeric nanoparticle covered with cellular membrane resulting from macrophages (represented PEG-Mac@NPs) have an antigenic external similar to the cells. The PEG-Mac@NPs, Isorhamnetin (Iso) on the free LPS encouraged endotoxin in BALB/c mice through evaluating the nitric acid, TNF-α, and IL-6. Further, the COX-2 and iNOS expression ratio was examined to recognize the connection of several trails to find the exact mode of action PEG-Mac@NPs and Iso. The outcome reveals that the PEG-Mac@NPs inhibited and LPS triggered the NO production though the macrophages peritoneal. Furthermore, the anti-inflammatory possessions were additionally categorized through the reduction of COX-2 and iNOS protein expressions. Engaging PEG-Mac@NPs as a biomimetic decontamination approach displays potential for refining sepsis patient consequences, possibly in the use of sepsis management.

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Acknowledgement

This study was supported by Wenzhou Science and Technology Project (No. Y20170755).

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Authors and Affiliations

  1. Department of Critical Care Medicine, Wenzhou Central Hospital, No. 252, Baili East Road, Lucheng District, Wenzhou, 325000, China

    Beilei Wu, Li Lin & Xiaobo Wang

  2. Department of Traditional Chinese Medicine, Wenzhou Central Hospital, Wenzhou, 325000, China

    Fan Zhou

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Correspondence to Xiaobo Wang.

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Wu, B., Lin, L., Zhou, F. et al. Precise engineering of neutrophil membrane coated with polymeric nanoparticles concurrently absorbing of proinflammatory cytokines and endotoxins for management of sepsis. Bioprocess Biosyst Eng 43, 2065–2074 (2020). https://doi.org/10.1007/s00449-020-02395-5

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