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Improving poly-(γ-glutamic acid) production from a glutamic acid-independent strain from inulin substrate by consolidated bioprocessing

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Abstract

To excavate the application of Jerusalem artichoke on poly(γ-glutamic acid) (γ-PGA) production, a γ-PGA producing strain Bacillus amyloliquefaciens NX-2S154 was obtained through atmospheric and room temperature plasma mutagenesis, which produced 14.83 ± 0.31 g/L of γ-PGA in batch fermentation with raw inulin extract. Simultaneous saccharification and fermentation (SSF) by adding commercial inulinase were further investigated for γ-PGA fermentation. Results showed SSF could eliminate the ineffective utilization of inulin while avoiding inhibition effect of high concentration substrate, which made γ-PGA concentration reach 18.54 ± 0.39 g/L with the process being shortened by 17%. Finally, an immobilized column for reducing inulinase cost was introduced to γ-PGA production. Repeated batch cultures showed the novel bioreactor exhibited higher stability and simplicity and gave average γ-PGA concentration and productivity of 19.40 ± 0.37 g/L and 0.27 ± 0.008 g/L/h, respectively. This work proposes a productive method for efficient γ-PGA production using Jerusalem artichoke feedstock.

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Acknowledgments

This work was funded by the National Nature Science Foundation of China (No. 21878152; No. 21506098; No. 21776133), the Nanchang Hongcheng Specialist Project, the Six Talent Peaks Project in Jiangsu Province (2016-SWYY-027), Graduate Student Innovation Project of Jiangsu Province (No. KYCX18_1112).

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Authors and Affiliations

  1. State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing, 211816, China

    Yibin Qiu, Yatao Zhang, Yifan Zhu, Yuanyuan Sha, Zongqi Xu, Xiaohai Feng, Sha Li & Hong Xu

  2. College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211816, China

    Yibin Qiu, Yatao Zhang, Yifan Zhu, Yuanyuan Sha, Zongqi Xu, Xiaohai Feng, Sha Li & Hong Xu

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  1. Yibin Qiu
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  8. Hong Xu
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Correspondence to Hong Xu.

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Qiu, Y., Zhang, Y., Zhu, Y. et al. Improving poly-(γ-glutamic acid) production from a glutamic acid-independent strain from inulin substrate by consolidated bioprocessing. Bioprocess Biosyst Eng 42, 1711–1720 (2019). https://doi.org/10.1007/s00449-019-02167-w

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