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Kinetic identification of phenolic compounds and potential production of caffeic acid by Ganoderma lipsiense in solid-state fermentation

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Abstract

Basidiomycetes fungi have been utilized for the production of several compounds with bioactive properties, such as phenolic compounds. The present work quantified and identified the phenolic compounds produced in a kinetic study (63 days) and evaluated the antimicrobial activity from the extract obtained by Ganoderma lipsiense cultivation in solid-state fermentation using red rice. Phenolic compounds were identified by high-performance liquid chromatography–electrospray ionization tandem mass spectrometry (HPLC–ESI–MS/MS) and caffeic acid content was measured by high-performance liquid chromatography with diode-array detection (HPLC–DAD). Caffeic and syringic acids were produced by G. lipsiense. In the control medium (red rice), the following compounds were identified: p-coumaric acid, salicylic acid, ferulic acid and vanillin. High concentrations of caffeic acid (0.977 µg g−1) were measured in 49 days. Antimicrobial activity was investigated against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus using a minimum inhibitory concentration (MIC) technique. Ganoderma lipsiense extract was only effective against P. aeruginosa. These data have proved to be satisfactory in the study of biosynthesis of caffeic acid and antibacterial compounds by G. lipsiense in solid-state fermentation with red rice.

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Acknowledgements

The authors are grateful to the Coordination for the Improvement of Higher Education Personnel (CAPES) Brazil, for financial support (Code 001). The authors D. Oliveira and L.B.B. Tavares are fellowship holders of the National Council for Scientific and Technological Development (CNPq).

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Authors and Affiliations

  1. Department of Chemical Engineering, Federal University of Santa Catarina, Trindade, Florianópolis, Santa Catarina, CEP 88040-900, Brazil

    Tania Maria Costa & Débora de Oliveira

  2. Graduate Program in Environmental Engineering, Regional University of Blumenau, Itoupava Seca, Blumenau, Santa Catarina, CEP 89030-080, Brazil

    Vander Kaufmann & Lorena Benathar Ballod Tavares

  3. Chemistry Department, Regional University of Blumenau, Itoupava Seca, Blumenau, Santa Catarina, CEP 89030-080, Brazil

    Camila Jeriane Paganelli & Michele Debiasi Alberton

  4. Chemistry Department, Federal University of Santa Catarina, Trindade, Florianópolis, Santa Catarina, CEP 88040-900, Brazil

    Diogo Alexandre Siebert & Gustavo Amadeu Micke

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  1. Tania Maria Costa
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Costa, T.M., Kaufmann, V., Paganelli, C.J. et al. Kinetic identification of phenolic compounds and potential production of caffeic acid by Ganoderma lipsiense in solid-state fermentation. Bioprocess Biosyst Eng 42, 1325–1332 (2019). https://doi.org/10.1007/s00449-019-02131-8

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