Abstract
Whole cells of Escherichia coli overexpressing a glucosyltransferase from Vitis vinifera were used for the glucosylation of geraniol to geranyl glucoside. A high cell density cultivation process for the production of whole-cell biocatalysts was developed, gaining a dry cell mass concentration of up to 67.6 ± 1.2 g L−1 and a glucosyltransferase concentration of up to 2.7 ± 0.1 g protein L−1 within a process time of 48 h. Whole-cell batch biotransformations in milliliter-scale stirred-tank bioreactors showed highest conversion of geraniol at pH 7.0 although the pH optimum of the purified glucosyltransferase was at pH 8.5. The biocatalytic batch process performance was improved significantly by the addition of a water-immiscible ionic liquid (N-hexylpyridinium bis(trifluoromethylsulfonyl)imid) for in situ substrate supply. The so far highest final geranyl glucoside concentration (291 ± 9 mg L−1) and conversion (71 ± 2 %) reported for whole-cell biotransformations of geraniol were achieved with 5 % (v/v) of the ionic liquid.
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Acknowledgments
The authors gratefully acknowledge the support of Andreas Schmideder by the TUM Graduate School (Technische Universität München, Germany). The support of Xenia Priebe by the International Graduate School of Science and Engineering (Technische Universität München, Germany) is acknowledged as well. We are thankful for the support of Fong-Chin Huang by the FLÜGGE program.
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Schmideder, A., Priebe, X., Rubenbauer, M. et al. Non-water miscible ionic liquid improves biocatalytic production of geranyl glucoside with Escherichia coli overexpressing a glucosyltransferase. Bioprocess Biosyst Eng 39, 1409–1414 (2016). https://doi.org/10.1007/s00449-016-1617-6
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DOI: https://doi.org/10.1007/s00449-016-1617-6