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Cell growth stimulating effect of Ganoderma lucidum spores and their potential application for Chinese hamster ovary K1 cell cultivation

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Abstract

In this work, water-soluble extracts of Ganoderma lucidum spores (Gls), a Chinese medicinal herb that possesses cell growth stimulating function, were found to be an effective growth factor for Chinese hamster ovary (CHO) cell cultivation. The Gls extract was prepared and supplemented to CHO K1 cell culture media with various serum levels. Our results obtained from both the static culture and the spinner-flask suspension culture showed that use of small-amount Gls extract effectively promoted cell growth and suppressed cell apoptosis induced by serum deprivation with normal cell cycle maintained in a low-serum medium. The low-serum medium containing 1 % (v/v) fetal bovine serum (FBS) and 0.01 % (w/v) Gls extract showed a comparable performance on both cell growth and fusion protein productivity with the conventional CHO culture medium containing 10 % (v/v) FBS and a commercial serum-free medium. This is the first study of the potential of Gls extracts for use as an alternative cell growth factor and nutrient for CHO cells. The findings have presented a new approach to economic cultivation of CHO cells for therapeutic protein production.

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Acknowledgments

This research was supported by National High Technology Research and Development Program of China (2014AA021004).

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  1. Ding Li

    Present address: Department of Cardiology, University of Louisville, Louisville, KY, 40202, USA

Authors and Affiliations

  1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China

    Ding Li, Qi Zhong, Tingting Liu & Jufang Wang

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  1. Ding Li
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  2. Qi Zhong
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Correspondence to Ding Li or Jufang Wang.

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The authors declare that they have no conflicts of interest in the research.

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D. Li and Q. Zhong contributed equally to this study.

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Li, D., Zhong, Q., Liu, T. et al. Cell growth stimulating effect of Ganoderma lucidum spores and their potential application for Chinese hamster ovary K1 cell cultivation. Bioprocess Biosyst Eng 39, 925–935 (2016). https://doi.org/10.1007/s00449-016-1572-2

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