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Continuous synthesis of hexanal by immobilized hydroperoxide lyase in packed-bed reactor

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Abstract

This study aimed to develop an optimal continuous procedure of immobilized hydroperoxide lyase (HPL)-catalyzed synthesis of hexanal. A central composite design was used to study the combined effect of substrate concentration and the residence time of the reactant on hexanal concentration. The optimum conditions for hexanal synthesis included a 13-HPOD concentration of 43.54 mM and a residence time of 60.99 min. The maximum hexanal concentration was 3560 ± 130 mg/L when 16 U of immobilized HPLwas used. Furthermore, the stability of immobilized HPL was significantly improved in the packed-bed reactor, as evidenced by the slowed enzyme inactivation and prolonged operation time. The immobilized HPL remained activity until 40 mL substrate solution flowed past the packed-bed reactor. The catalyst productivity of hexanal in the packed-bed reactor was 5.35 ± 0.34 mg/U, much higher than that in the batch stirred reactor. This study was greatly meaningful for providing a green method to the large-scale production of hexanal.

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  1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, People’s Republic of China

    Qingqing Liu & Yufei Hua

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  1. Qingqing Liu
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  2. Yufei Hua
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Correspondence to Yufei Hua.

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Liu, Q., Hua, Y. Continuous synthesis of hexanal by immobilized hydroperoxide lyase in packed-bed reactor. Bioprocess Biosyst Eng 38, 2439–2449 (2015). https://doi.org/10.1007/s00449-015-1481-9

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