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Improvement of catalytic activity of lipase in the presence of calix[4]arene valeric acid or hydrazine derivative

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Abstract

Sol–gel encapsulation is a simple but powerful method to enhance the enantioselectivity of lipase-catalyzed transformations in an isooctane/aqueous buffer solution. Candida rugosa lipase was encapsulated according to a sol–gel procedure in the presence and absence of calix[4]arene hydrazine or carboxylic acid derivatives with Fe3O4 magnetic nanoparticles as an additive. The activity of the encapsulated lipases was evaluated for the enantioselective hydrolysis of racemic Naproxen methyl ester and the hydrolysis of p-Nitrophenylpalmitate. The results indicate that the encapsulated lipase without calix[4]arene derivative has lower conversion and enantioselectivity compared to the encapsulated lipase with calix[4]arene derivative. It was found that the calix[4]arene hydrazine and carboxylic acid-based encapsulated lipases have excellent activity and enantioselectivity (E >300) compared to encapsulated lipase without the calix[4]arene derivatives.

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Acknowledgments

We would like to thank the Scientific and Technological Research Council of Turkey (TUBITAK Grant Number 111T027) and the CMST COST Action No: CM1005 and the Research Foundation of Selcuk University (BAP) for financial support of this work.

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Authors and Affiliations

  1. Department of Chemistry, Selcuk University, 42031, Konya, Turkey

    Enise Akoz, Serkan Sayin, Selcuk Kaplan & Mustafa Yilmaz

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  1. Enise Akoz
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  2. Serkan Sayin
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  3. Selcuk Kaplan
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  4. Mustafa Yilmaz
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Correspondence to Mustafa Yilmaz.

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Akoz, E., Sayin, S., Kaplan, S. et al. Improvement of catalytic activity of lipase in the presence of calix[4]arene valeric acid or hydrazine derivative. Bioprocess Biosyst Eng 38, 595–604 (2015). https://doi.org/10.1007/s00449-014-1299-x

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